LOCUS       sequence-34325-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Sun Q, Zhang J, Fan W, Wong KN, Ding X, Chen S, Zhong Q
  TITLE     The RUN domain of rubicon is important for hvps34 binding, lipid 
            kinase inhibition, and autophagy suppression.
  JOURNAL   J Biol Chem. 2011 Jan 7. 286(1):185-91.
  PUBMED    21062745
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jan 7. 286(1):185-91."
COMMENT     Sequence Label: EGFP-Rubicon
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..79)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tagactcgag tcacttgtca tcgtcatcct tgtagtcgat gtcatgatct ttataatcac
       61 cgtcatggtc tttgtagtcg cggccgccgg tggcctccag gacggcggct tccagggcca
      121 gcgcttccgc gggctcctcc tcgtagtctg acaggtaaga ctccaggctc tgcctggcca
      181 gtgcctcccg ccgggcctgc agccgctcgc agcgcggaca gcttccagac ttgaagcagg
      241 ctttatggta acacgcttta cactcttcac aggtccggca cttatggagc tcaaagggaa
      301 agatgatgtc atcctcattc tgacagaact cacagatgaa gcctttggct tggcagagca
      361 tgcatctctc cacatgggta gcccctgccc tggtgagctc agcaagccgg ggccccagct
      421 cccccttcct ggtcgcagtc aggtcattca gtgagtacag gtggaggtcc tctgtcaggt
      481 ggcctgggac tgtgtcaaag gaatccagaa gctccttggc cagtcggcaa gtcttgaaca
      541 tgttcttcat gtgacacagc tggacccgca gcagccggac ttgattgagc agcttgacct
      601 tcctatagag ggcactgttt atgtcctgca cgttgaagag aggatcattc cagatcttaa
      661 tgagcaggtc cttggagaag ttgctgacgt agtacttgct gaagtcccac ttgcgcagaa
      721 cccggctggg gatggccatc tgggcattct cgtggcagca ctggcagaag tacttgccca
      781 ggtactcaca gtaccgcagt cgcttgatgt aatcagggtc agtccggatg ccacatcctg
      841 cacagcggta attctgcttg gccacggcaa ttttcctcgt tgg
//
