LOCUS       sequence-343262-        9549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Full-length YTHDC1 tagged with N-terminal 
            mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for 
            protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori
            EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, 
            Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, 
            Liu Z, Mancini MA, Li W
  TITLE     Enhancer RNA m6A methylation facilitates transcriptional condensate 
            formation and gene activation.
  JOURNAL   Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 
            10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
  PUBMED    34375583
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.024"; journalName: "Mol Cell"; date: 
            "2021-08-19- 19"; volume: "81"; issue: "16"; pages: "3368-3385.e9"
COMMENT     Sequence Label: pTXBI-mclover-YTHDC1-FL
FEATURES             Location/Qualifiers
     source          1..9549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(142..333)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(890..1972)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1934..1953
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1973..2050)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      2203..2289
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2386..2472
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2569..2655
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2752..2838
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2935..3021
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3156..3175
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3156..3174
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3175..3199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(3243..3245,3246..3248,3249..3959)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3288..3309)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3896..3917
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6138..6731
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             6762..6917
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(6982..7000)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6996..7043
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        7103..7207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7208..7276,7277..8068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7426..7445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8110..8623)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8305..8326)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8517..8536
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      8734..9322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9223..9242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9476..9493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9515..9534)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
       61 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      121 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
      181 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc
      241 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
      301 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
      361 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
      421 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
      481 gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc
      541 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
      601 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
      661 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
      721 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
      781 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
      841 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
      901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      961 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
     1021 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
     1081 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     1141 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     1201 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     1261 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     1321 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     1381 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     1441 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     1501 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
     1561 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
     1621 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
     1681 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
     1741 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
     1801 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
     1861 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
     1921 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
     1981 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
     2041 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
     2101 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc
     2161 gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct
     2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2341 gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag
     2401 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     2461 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     2521 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga
     2581 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2641 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     2701 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa
     2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2821 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     2881 gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata
     2941 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     3001 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     3061 ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt
     3121 cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt
     3181 gtgagcggat aacaattccc ctctagaaat aattttgtta actttaagaa ggagatatac
     3241 atatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3301 acggcgacgt aaacggccac aagttcagcg tccgcggcga gggcgagggc gatgccacca
     3361 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3421 ccctcgtgac caccttcggc tacggcgtgg cctgcttcag ccgctacccc gaccacatga
     3481 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3541 ctttcaagga cgacggtacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3601 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3661 acaagctgga gtacaacttc aacagccaca acgtctatat cacggccgac aagcagaaga
     3721 acggcatcaa ggctaacttc aagatccgcc acaacgttga ggacggcagc gtgcagctcg
     3781 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3841 actacctgag ccatcagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3901 tcctgctgga gttcgtgacc gccgccggga ttacacatgg catggacgag ctgtacaagg
     3961 cggctgacag tcgggaggag aaagatggag aacttaatgt tctggatgat attttaactg
     4021 aagtaccaga acaagatgat gaactgtata atccagagag tgaacaagat aaaaatgaga
     4081 aaaagggatc aaaaagaaaa agtgatcgaa tggaatctac tgataccaaa cgacaaaagc
     4141 cttctgtcca ttcaagacaa ctggtttcta agccactgag ctcatctgtt agcaataaca
     4201 aaagaatagt tagtacaaaa ggaaagtcag ccacagagta taaaaatgag gaatatcaaa
     4261 gatctgaaag aaacaagcgt ctagatgctg atcggaaaat tcgtctatca agtagtgcct
     4321 ccagagaacc ttataagaat caacctgaaa aaacctgtgt ccggaaaagg gatcctgaaa
     4381 ggagggccaa atctcctacg ccagatggtt ctgagagaat tgggcttgaa gtggatagac
     4441 gtgcaagcag atccagccag tcttctaagg aagaagtgaa ctctgaagaa tatggctctg
     4501 accatgagac tggcagcagt ggttcttctg atgagcaagg gaacaacact gagaatgagg
     4561 aggaaggagt ggaagaagat gtggaggaag atgaagaagt agaagaagat gcagaagaag
     4621 atgaagaggt ggatgaagat ggagaggagg aggaggaaga ggaggaggag gaagaggagg
     4681 aggaggagga ggaagaagaa gaatatgaac aggatgagag agaccagaaa gaggagggaa
     4741 atgattatga cactcgaagt gaggccagtg actctggttc tgaatctgtt tccttcacag
     4801 atgggtctgt cagatctggt tcaggcacag atggatcaga tgagaaaaag aaggaaagga
     4861 agagagctag aggcatatct ccaattgttt ttgatagaag tggaagctct gcatcagagt
     4921 catatgcagg ttcagaaaag aagcatgaga aattatcatc ttccgttcgt gctgtccgaa
     4981 aagatcaaac cagtaaactc aaatatgtgc ttcaagatgc aagatttttc ctcataaaga
     5041 gtaacaacca tgagaatgtg tctcttgcca aagcgaaggg tgtatggtcc acgctccctg
     5101 taaatgagaa gaaattaaat cttgcattta gatctgcaag gagtgttatc ttaatatttt
     5161 ctgtcagaga gagtggaaaa tttcaagggt ttgcaagact ttcttcagaa tcacatcacg
     5221 gaggatctcc tatacactgg gtgcttccag caggaatgag tgctaaaatg ctgggaggtg
     5281 tctttaaaat tgactggatt tgcaggcgtg aattaccctt cactaagtcg gctcatctca
     5341 ccaatccttg gaatgaacat aaaccagtaa agatcggacg tgatggacag gaaattgaac
     5401 ttgaatgtgg aacccagctt tgtcttctgt ttccccccga tgaaagtatt gacttgtatc
     5461 aggtcattca taaaatgcgt cacaagagaa gaatgcattc tcagccccga tcacgaggac
     5521 gtccatcccg tcgagaacca gtccgggatg tgggaaggcg tcgaccagaa gattatgata
     5581 ttcataacag cagaaagaaa ccaaggattg actatccccc tgagtttcac cagagaccag
     5641 ggtatttaaa ggatccacga taccaggaag tggacagacg attttcagga gttcgccgag
     5701 atgtgttttt aaatgggtcc tacaatgatt atgtgaggga atttcataac atgggaccac
     5761 caccaccttg gcaaggaatg cccccttacc caggaatgga acaacctcca caccatcctt
     5821 actatcagca ccatgctcca cctcctcaag ctcatccccc ttactcagga catcatccag
     5881 taccacatga agcaagatac agagataaac gagtacatga ttatgatatg agggtggatg
     5941 atttccttcg tcgcacacaa gctgttgtca gtggccggag aagtagaccc cgtgaaagag
     6001 accgggaacg agagcgagac cgccctagag ataacagacg agacagagag cgagatagag
     6061 gacgtgatag agaaagagaa agagagcgat tatgtgatcg agacagagac cgaggggaga
     6121 gaggtcgata tagaagatgc atcacgggag atgcactagt tgccctaccc gagggcgagt
     6181 cggtacgcat cgccgacatc gtgccgggtg cgcggcccaa cagtgacaac gccatcgacc
     6241 tgaaagtcct tgaccggcat ggcaatcccg tgctcgccga ccggctgttc cactccggcg
     6301 agcatccggt gtacacggtg cgtacggtcg aaggtctgcg tgtgacgggc accgcgaacc
     6361 acccgttgtt gtgtttggtc gacgtcgccg gggtgccgac cctgctgtgg aagctgatcg
     6421 acgaaatcaa gccgggcgat tacgcggtga ttcaacgcag cgcattcagc gtcgactgtg
     6481 caggttttgc ccgcgggaaa cccgaatttg cgcccacaac ctacacagtc ggcgtccctg
     6541 gactggtgcg tttcttggaa gcacaccacc gagacccgga cgcccaagct atcgccgacg
     6601 agctgaccga cgggcggttc tactacgcga aagtcgccag tgtcaccgac gccggcgtgc
     6661 agccggtgta tagccttcgt gtcgacacgg cagaccacgc gtttatcacg aacgggttcg
     6721 tcagccacgc tactggcctc accggtctga actcaggcct cacgacaaat cctggtgtat
     6781 ccgcttggca ggtcaacaca gcttatactg cgggacaatt ggtcacatat aacggcaaga
     6841 cgtataaatg tttgcagccc cacacctcct tggcaggatg ggaaccatcc aacgttcctg
     6901 ccttgtggca gcttcaatga ctgcaggaag gggatccggc tgctaacaaa gcccgaaagg
     6961 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     7021 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggataa ctacgtcagg
     7081 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7141 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7201 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7261 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7321 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7381 tcgccccgaa gaacgtttcc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7441 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7501 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7561 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7621 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7681 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7741 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7801 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7861 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7921 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7981 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8041 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8101 gattgattta ccccggttga taatcagaaa agccccaaaa acaggaagat tgtataagca
     8161 aatatttaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8221 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8281 cccgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8341 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8401 tcacccaaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8461 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8521 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8581 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtaaaagga tctaggtgaa
     8641 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8881 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9121 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9181 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9241 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9301 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9361 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9421 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9481 gtcagtgagc gaggaagcta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9541 taagccagt
//
