LOCUS       sequence-343307-        4065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This gene was cloned into the gateway middle entry vector. It can be
            used for generating antisense riboprobe or gene expression using a 
            gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Silic MR, Murata SH, Park SJ, Zhang G
  TITLE     Evolution of inwardly rectifying potassium channels and their gene 
            expression in zebrafish embryos.
  JOURNAL   Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425.
  PUBMED    34558132
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Sep 23. doi: 10.1002/dvdy.425."
COMMENT     Sequence Label: pDONR221-kcnj19a
FEATURES             Location/Qualifiers
     source          1..4065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2190..2289)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2306..2325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2307..2325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2330..2346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2330..2346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2448..2467)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2459..3268
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2526..2545)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3415..4003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3904..3923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc accatggcag cgctaaggcg taaatttggc gaggactacc aggtggtgaa
      721 caccaacaga ggcgcgactt tctccagcgc accggtgaag aaaaagcgcc agcgcttcgt
      781 ggagaaaaac ggccgctgca acgtccagca cggaaacctc ggtggagaaa ccagccgcta
      841 tttatcagac ctgttcacca cactagtgga cttgaagtgg cgctggaacc ttctcatttt
      901 tattctcacg tacaccatcg cctggctggt catggcctcg atgtggtgga tcatcgcgta
      961 tataagaggg gacttgaatc acggccacga cgcttcttac actccgtgcg tcgccaatgt
     1021 ttacaacttc ccctccgcgt tcctcttctt cattgagaca gaggcgacca ttggctacgg
     1081 ttaccgctat ataaccgaga agtgccctga gggcatcatc ctgttcctgt tccagtctct
     1141 actgggctcg atagtggacg cgtttctcat cggctgtatg ttcattaaga tgtctcagcc
     1201 caagaagcgc gcggagactc tcatgttcag tcaggacgcg gtcatttcac agcgagacgg
     1261 aaaactctgc ttaatgttcc gcgtgggcaa tctgaggaac agccatatgg tgtccgcgca
     1321 aatcaggtgt aaactcataa agtctcgtca gacaccggag ggtgagttcc tgccattgga
     1381 tcagtgtgaa ctggatgtcg gttttgggac aggtgcagat cagctcttct tggtctctcc
     1441 cctcaccatc tgccacgaga tcaatcccaa gagccccttc tttgacctgt cccagcgttc
     1501 gctcatgaac gagcagtttg agatcgtggt catcttggag ggtattgtag agacgacggg
     1561 tatgacgtgt caagctcgta cttcatacac ggaggatgag gtgttgtggg gtcacagatt
     1621 cctgcctgtc atgtctctgg aagagggctt tttcagggtg gactactccc agtttcacag
     1681 caccttcgag gtgcccacac caccctacag cgtcaaagaa tacgaggaga aatcttcact
     1741 cccttcgccc ctgctcaccc cacaccggga gcgagtcttt tccatggact gtctggaaac
     1801 aggtgacgaa ggcctgcatc ccggaagcgc ttcatgtaag ttgcccagta aactccagaa
     1861 aatgagttcg tctggcagag atgatctcca gaggaaggtg ttacgcttga gctcgcagca
     1921 gtctgaaaaa gcctcaagca caggagacct tcctcttaaa ctgcagcgcc tcagctctag
     1981 tcccgggcag gttgacggag aggcacggtt ccacctcaaa gccctgaagg taggtttcca
     2041 gcccatggcc ttgtccactg gagacctcca ccagaggagt ctgcctccca cgccagcagt
     2101 ggtcccaaca caccctccac acacacccag ctgcaggcct gaagacaacc tgcctgccaa
     2161 actgaggagg atgaatgcag accgctgaga cccagctttc ttgtacaaag ttggcattat
     2221 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2281 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2341 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2401 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2461 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     2521 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     2581 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     2641 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     2701 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     2761 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2821 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2881 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2941 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3001 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3061 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3121 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3181 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3241 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3301 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3361 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
