LOCUS       sequence-343317-        9143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Improved green-red FRET based cAMP indicator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Mo GCH, Posner C, Rodriguez EA, Sun T, Zhang J
  TITLE     A rationally enhanced red fluorescent protein expands the utility of
            FRET biosensors.
  JOURNAL   Nat Commun. 2020 Apr 15;11(1):1848. doi: 10.1038/s41467-020-15687-x.
  PUBMED    32296061
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-15"; volume: "11(1):1848. doi"; pages: " 
            10.1038/s41467-020-15687-x"
COMMENT     Sequence Label: pcDNA3-GR-ICUE2
FEATURES             Location/Qualifiers
     source          1..9143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(58..162)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(237..256)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        428..807
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        808..1011
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     962..982
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1056..1075
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1056..1074
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1094..1103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1115..1132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1136..1168
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1172..1180,1181..1195)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(1202..1204,1205..1207,1208..1918)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1247..1268)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1508..1527)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1855..1876
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4130..4825
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTVMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     promoter        complement(4889..4907)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4890..4907)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4927..4944)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4933..5157
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5203..5631
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5290..5309)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5500..5521
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5640..5660)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5645..5974
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5825..5960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5887..5906
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6041..6835
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6095..6114)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6705..6724
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7009..7130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7046..7065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7100..7119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7179..7195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7179..7195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7192..7214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7203..7219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7227..7233,7234..7251,7252..7257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7272..7293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7410..7427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7581..8169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7661..7680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8340..9131,9132..9143,1..57))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8963..8982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
       61 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
      121 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
      181 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
      241 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
      301 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
      361 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      421 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      481 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      541 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      601 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      661 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      721 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      781 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      841 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      901 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      961 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     1021 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
     1081 caagcttgcg gccgccacca tggtgcgggg ttctcatcat catcatcatc atggtatggc
     1141 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc
     1201 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1261 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1321 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1381 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1441 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1501 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1561 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1621 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     1681 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1741 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1801 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     1861 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag
     1921 atctcccgtg ggaactcatg agatggagga ggagttggcc gaagctgtgg ccctgctctc
     1981 ccagcggggg cctgacgccc tgctcactgt ggcacttcga aagcccccag gtcagcgcac
     2041 ggatgaagag ctggacctca tctttgagga gctgctgcac atcaaggctg tggcccacct
     2101 ctccaactcg gtgaagcgag aattagcggc tgttctgctc tttgaaccac acagcaaggc
     2161 agggaccgtg ttgttcagcc agggggacaa gggcacttcg tggtacatta tctggaaggg
     2221 atctgtcaac gtggtgaccc atggcaaggg gctggtgacc accctgcatg agggagatga
     2281 ttttggacag ctggctctgg tgaatgatgc accccgggca gccaccatca tcctgcgaga
     2341 agacaactgt catttcctgc gtgtggacaa gcaggacttc aaccgtatca tcaaggatgt
     2401 ggaggcaaag accatgcggc tggaagaaca tggcaaagtg gtgctggtgc tggagagagc
     2461 ctctcagggc gccggccctt cccgaccccc aaccccaggc aggaaccggt atacagtgat
     2521 gtctggcacc ccagagaaga tcctagagct tctgttggag gccatgggac cagattccag
     2581 tgctcatgac caaacagaga cattcctcag cgacttcctc ctgacccaca gggtcttcat
     2641 gcccagcgcc caactctgcg ctgcccttct gcaccacttc catgtggagc ctgcgggtgg
     2701 cagcgagcag gagcgcagca cctacgtctg caacaagagg cagcagatct tgcggctggt
     2761 cagccagtgg gtggccctgt atggctccat gctccacact gaccctgtgg ccaccagctt
     2821 cctccagaaa ctctcagacc tggtgggcag ggacacccga ctcagcaacc tgctgaggga
     2881 gcagtggcca gagaggcggc gatgccacag gttggagaat ggctgtggga atgcatctcc
     2941 tcagatgaag gcccggaact tgcctgtttg gctccccaac caggacgagc cccttcctgg
     3001 cagcagctgt gccatccaag ttggggataa agtcccctat gacatctgcc ggccagacca
     3061 ctcagtgttg accctgcagc tgcctgtgac agcctccgtg agagaggtga tggcagcgtt
     3121 ggcccaggag gatggctgga ccaaggggca ggtgctggtg aaggtcaatt ctgcaggtga
     3181 tgccattggc ctgcagccag atgcccgtgg tgtggccaca tctctggggc tcaatgagcg
     3241 tctctttgtt gtcaacccac aggaagcgca tgagctgatc ccacaccctg accagctggg
     3301 gcccactgtg ggctctgctg aggggctgga cctggtgagt gccaaggacc tggcaggcca
     3361 gctgacggac cacgactgga gcctcttcaa cagtatccac caggtggagc tgatccacta
     3421 tgtgctgggc ccccagcatc tgcgggatgt caccaccgcc aacctggagc gcttcatgcg
     3481 ccgcttcaat gagctgcagt actgggtggc caccgagctg tgtctctgcc ccgtgcccgg
     3541 cccccgggcc cagctgctca ggaagttcat taagctggcg gcccacctca aggagcagaa
     3601 gaatctcaat tccttctttg ccgtcatgtt tggcctcagc aactcggcca tcagccacct
     3661 agcccacacc tgggagcggc tgccccacaa agtccggaag ctgtactccg ccctcgagag
     3721 gctgctggac ccctcatgga accaccgggt ataccgactg gccctcgcca agctctcccc
     3781 tcctgtcatc cccttcatgc cccttcttct caaagacatg accttcattc atgagggaaa
     3841 ccacacacta gtggagaatc tcatcaactt tgagaagatg agaatgatgg ccagagtcgc
     3901 gcggatgctg caccactgcc gaagccacaa ccctgtgcct ctctcaccac tcagaagccg
     3961 agtttcccac ctccacgagg acagccaggt ggcgaggatt tccacatgct cggagcagtc
     4021 cctgagcacc cggagtccag ccagcacctg ggcttatgtc cagcagctga aggtcattga
     4081 caaccagcgg gaactctccc gcctctcccg agagctggag ccaggtacca tgagcgagct
     4141 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     4201 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     4261 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     4321 cggcagcaga accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt
     4381 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     4441 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     4501 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     4561 cgagatgctg taccccgctg acggcggcct ggaaggcaga accgtcatgg ccctgaagct
     4621 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     4681 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     4741 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     4801 ccctagcaaa ctggggcaca agtaagaatt caagaagaag aagaagagca agaccaagtg
     4861 cgtgatcatg taatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct
     4921 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4981 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5041 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     5101 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     5161 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     5221 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5281 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5341 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5401 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5461 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5521 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5581 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     5641 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5701 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5761 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5821 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5881 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5941 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     6001 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     6061 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     6121 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     6181 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     6241 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     6301 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     6361 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     6421 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     6481 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     6541 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     6601 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     6661 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6721 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6781 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     6841 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6901 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6961 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     7141 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7981 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9121 gtttctgggt gagcaaaaac agg
//
