LOCUS       sequence-34332-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Sun Q, Westphal W, Wong KN, Tan I, Zhong Q
  TITLE     Rubicon controls endosome maturation as a Rab7 effector.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.
  PUBMED    20974968
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Nov 9. 107(45):19338-43."
COMMENT     Sequence Label: Flag-Rab5A
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..79)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(842..866)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tagactcgag tcacttgtca tcgtcatcct tgtagtcgat gtcatgatct ttataatcac
       61 cgtcatggtc tttgtagtcg cggccgccgt tactacaaca ctgattcctg gttggttgtg
      121 tgggttcggt aaggtctact cctcttcctc tggcagaatt tgctcctgga ttttgtggtt
      181 cattctttgg caatttttta gctattgcca tgaatatttc atttacattc attgatgttt
      241 tagcggatgt ctccatgaat aataaactat tgtcatctgc ataggactgt gcttcctgga
      301 aatctactgc tcttttattt gctaggtcgg ccttgtttcc cgataaagct attacaatgt
      361 taggacttgc ttgcctctga agttctttaa cccaattttt tgctcttgca aaggactcct
      421 catttgtgat atcatataca actatggctg cttgtgctcc tctgtagtac attggtgcta
      481 ggctatggta tcgttcttga ccagctgtat cccatatttc aaactttact gtagtgtcat
      541 caagacatac agtttgggtt agaaaagcag ccccaatggt actctcttga aattcatgaa
      601 attggccttt cacaaaacga agcactaggc ttgatttgcc aacagcggac tctcccagaa
      661 gtactagttt gaactggcat attttatttc cagtatttgg cccgttgggt cttgttgcgc
      721 ctcgactagc catggatccg agctcggtac caagcttaag tttaaacgct agagtccgga
      781 ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
      841 cgatctgacg gttcactaaa cgagctcgtc gac
//
