LOCUS       sequence-343353-        4798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MusF2 prenyltransferases from Nmu. PCC 7906.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Mattila A, Andsten RM, Jumppanen M, Assante M, Jokela J, Wahlsten M,
            Mikula KM, Sigindere C, Kwak DH, Gugger M, Koskela H, Sivonen K, Liu
            X, Yli-Kauhaluoma J, Iwai H, Fewer DP
  TITLE     Biosynthesis of the Bis-Prenylated Alkaloids Muscoride A and B.
  JOURNAL   ACS Chem Biol. 2019 Dec 20;14(12):2683-2690. doi: 
            10.1021/acschembio.9b00620. Epub 2019 Nov 19.
  PUBMED    31674754
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.9b00620"; journalName: "ACS Chem Biol"; date: 
            "2019-12-20- 20"; volume: "14"; issue: "12"; pages: "2683-2690"
COMMENT     Sequence Label: pTVKRSF225
FEATURES             Location/Qualifiers
     source          1..4798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(104..1186)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1148..1167
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1187..1264)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1310..1329
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1310..1328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1329..1353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1409..1426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1433..1723
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             2661..2705
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(2743..2761)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2757..2804
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3034..3849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3034..3849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3757..3776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3850..3941)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3957..4706)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(3967..3984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4207..4226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
       61 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
      121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      181 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
      241 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
      301 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
      361 tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg
      421 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
      481 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
      541 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
      601 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
      661 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
      721 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
      781 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
      841 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
      901 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
      961 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     1021 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     1081 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     1141 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     1201 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     1261 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc
     1321 actatagggg aattgtgagc ggataacaat tcccctgtag aaataatttt gtttaacttt
     1381 aataaggaga tataccatgg gcagcagcca tcatcatcat catcacagca ctagtgactc
     1441 agaagtcaat caagaagcta agccagaggt caagccagaa gtcaagcctg agactcacat
     1501 caatttaaag gtgtccgatg gatcttcaga aatcttcttt aagatcaaaa agaccactcc
     1561 tttaagaagg ctgatggaag cgttcgctaa aagacagggt aaggaaatgg actccttaag
     1621 attcttgtac gacggtatta gaattcaagc tgatcagacc cctgaagatt tggacatgga
     1681 ggataacgat attattgagg ctcacagaga acagattggt ggatccgtaa cttataaacc
     1741 gattccaact gatagcgaac gctacttaga atacattcgt aaacatcaac ttgcctttga
     1801 tatttacgaa gacctctatc ccctgaaact ttttgaagat ttcgtgcaag cagagggtag
     1861 aaaagaaagt tggttttaca attgctgtaa tatagataaa gatcaattat atccagcccg
     1921 ctttaccttt ggctatcctc cccaaaaaga agctaaattg ctgcacaatc cccaacggca
     1981 actccaggaa gtctttcact tttttcgcca agtagaaaat cggccagaag taaagctcaa
     2041 ttatcgtttt attcaccagt tttttagcat tcatcctgat gtgcgtggca tttgctgggt
     2101 tggggttggg gtagatactc gcacggttat tactgaatct cgcctcaagc tatttattgg
     2161 actcaaggat gctcccgaca aggtagaaac tgccattact ctgtgtggag atagtcccac
     2221 gttacgagca tttttactca acgattacaa taattacttg ttaataggat ttgatttatt
     2281 ttttgacaat cacagcgaaa tcgagcttta tccgactatt tttgaagata agttacgact
     2341 atctaatgtc cgcagccaaa ttacaccact tttaccccct cgtgctttac cgttactcga
     2401 ccaatgtgaa gtttttcaaa tcggcattag tccagccaat gacagtaata ttctttactt
     2461 tgatgatgtt tacgatccca ataattttgt tgataatttt ggcaatgaga tggtcaaaaa
     2521 agttcatgcc tattaccgcc atcaacctat caaaaaattg ctaattggcg ctcctgaaaa
     2581 agacttttac gctcaatcaa ttgaacatgc gaagctgtat tataggatga attaagcttg
     2641 cggccgcact cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg
     2701 actcgtctac tagcgcagct taattaacct aggctgctgc caccgctgag caataactag
     2761 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat
     2821 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
     2881 cataagcggc tatttaacga ccctgccctg aaccgacgac aagctgacga ccgggtctcc
     2941 gcaagtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3001 cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg
     3061 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     3121 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     3181 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     3241 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     3301 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     3361 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcggtc
     3421 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     3481 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     3541 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     3601 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     3661 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     3721 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     3781 taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat
     3841 atggctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggcatgc agcgctcttc
     3961 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggtgtcagc
     4021 tcactcaaaa gcggtaatac ggttatccac agaatcaggg gataaagccg gaaagaacat
     4081 gtgagcaaaa agcaaagcac cggaagaagc caacgccgca ggcgtttttc cataggctcc
     4141 gcccccctga cgagcatcac aaaaatcgac gctcaagcca gaggtggcga aacccgacag
     4201 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4261 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4321 atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4381 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4441 ccaacccggt aagacacgac ttatcgccac tggcagcagc cattggtaac tgatttagag
     4501 gactttgtct tgaagttatg cacctgttaa ggctaaactg aaagaacaga ttttggtgag
     4561 tgcggtcctc caacccactt accttggttc aaagagttgg tagctcagcg aaccttgaga
     4621 aaaccaccgt tggtagcggt ggtttttctt tatttatgag atgatgaatc aatcggtcta
     4681 tcaagtcaac gaacagctat tccgttactc tagatttcag tgcaatttat ctcttcaaat
     4741 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcc
//
