LOCUS       sequence-343360-        8586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of human Meikin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Maier NK, Ma J, Lampson MA, Cheeseman IM
  TITLE     Separase cleaves the kinetochore protein Meikin at the meiosis I/II 
            transition.
  JOURNAL   Dev Cell. 2021 Aug 9;56(15):2192-2206.e8. doi: 
            10.1016/j.devcel.2021.06.019. Epub 2021 Jul 30.
  PUBMED    34331869
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.06.019"; journalName: "Dev Cell"; date: 
            "2021-08-9- 9"; volume: "56"; issue: "15"; pages: "2192-2206.e8"
COMMENT     Sequence Label: TetO::EGFP-hMeikin
FEATURES             Location/Qualifiers
     source          1..8586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(14..602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(94..113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(773..1564,1565..1633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1396..1415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1634..1738)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1775..2611
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    2619..2674
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(2703..3449)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             3472..3740
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3753..3964)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        4009..4387
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4017..4035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4053..4071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4089..4107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4125..4143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4161..4179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4197..4215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4233..4251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4296..4320
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4394..4403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4400..4402,4403..4405,4406..5116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4400..4402,4403..4405,4406..5116)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4445..4466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4706..4725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5053..5074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5162..5182
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5195..5239
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(6681..6698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6687..6911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    6928..7049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6939..6958)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6993..7012
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7080..7679)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7163..7183)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7660..7679
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(7686..7739)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    7745..7770
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    7777..8580
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ccttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
       61 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      121 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      181 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      241 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      361 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      421 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      481 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      661 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      721 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      781 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      841 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      901 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      961 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1021 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1081 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1141 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1201 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1261 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1321 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1381 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1441 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1561 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1621 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1681 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1741 catttccccg aaaagtgctt aagcccttgc ccttagagca gagccaggaa cccctgtagg
     1801 gaaggggcag gagagccagg ggcatgagat ggtggacgag gaagggggac agggaagcct
     1861 gagcgcctct cctgggcttg ccaaggactc aaacccagaa gcccagagca gggccttagg
     1921 gaagcgggac cctgctctgg gcggaggaat atgtcccaga tagcactggg gactctttaa
     1981 ggaaagaagg atggagaaag agaaagggag tagaggcggc cacgacctgg tgaacaccta
     2041 ggacgcacca ttctcacaaa gggagttttc cacacggaca cccccctcct caccacagcc
     2101 ctgccaggac ggggctggct actggcctta tctcacaggt aaaactgacg cacggaggaa
     2161 caatataaat tggggactag aaaggtgaag agccaaagtt agaactcagg accaacttat
     2221 tctgattttg tttttccaaa ctgcttctcc tcttgggaag tgtaaggaag ctgcagcacc
     2281 aggatcagtg aaacgcacca gacagccgcg tcagagcagc tcaggttctg ggagagggta
     2341 gcgcagggtg gccactgaga accgggcagg tcacgcatcc cccccttccc tcccaccccc
     2401 tgccaagctc tccctcccag gatcctctct ggctccatcg taagcaaacc ttagaggttc
     2461 tggcaaggag agagatggct ccaggaaatg ggggtgtgtc accagataag gaatctgcct
     2521 aacaggaggt gggggttaga cccaatatca ggagactagg aaggaggagg cctaaggatg
     2581 gggcttttct gtcaccaatc ctgtccctag taagagcttg agagacacaa aaaattccaa
     2641 cacactattg caatgaaaat aaatttcctt tattccggaa taaaggaaat ttagtacccg
     2701 ggttaccctg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca
     2761 aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc gtcaagggcg
     2821 tcggtcggcc cgccgctttc gcactttagc tgtttctcca ggccacatat gattagttcc
     2881 aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga acagctcaat tgcttgtttc
     2941 agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc tacttgatgc
     3001 tcctgttcct ccaatacgca gcccagtgta aagtggccca cggcggacag agcgtacagt
     3061 gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc cagggtttcg
     3121 tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg
     3181 agagcacagc ggtatgactt ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc
     3241 agggggcagg agtgggtatg atgcctgtcc agcatctcga ttggcagggc atcgagcagg
     3301 gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct caactcccag cttttgagcg
     3361 agtttccttg tcgtcaggcc ttcgataccg actccattga gtaattccag agcagagttt
     3421 atgactttgc tcttgtccag tctagacatg gtgcttaatt aacacagctt ggatctggaa
     3481 cggcgcagaa cagaaaacga aacaaagacg tagagttgag caagcagggt caggcaaagc
     3541 gtggagagcc ggctgagtct aggtaggctc caagggagcg ccggacaaag gcccggtctc
     3601 gacctgagct ttaaacttac ctagacggcg gacgcagttc aggaggcacc tcaggcggga
     3661 ggcggcagaa cgcgactcaa ccggcgtgga tggcggcctc aggtagggcg gcgggcgcgt
     3721 gaaggagaga tgcgagcccc tcgaagcttc agctgtgttc tggcggcaaa cccgttgcga
     3781 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
     3841 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
     3901 cccgttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
     3961 gcccactgac gggcaccgga gagatctttt cgtcttcaac aattcttcga gtttactccc
     4021 tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag aacgtatgca
     4081 gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta tcagtgatag
     4141 agaacgtata tccagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
     4201 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
     4261 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
     4321 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     4381 tcgtaaagtc gacgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4441 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4501 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4561 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4621 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4681 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4741 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4801 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4861 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4921 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4981 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5041 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5101 ggacgagctg tacaagtccg gaggggatcg gtggagctcc accggtggcg gccgctctag
     5161 agagaatctt tattttcagg gcgccgccaa attcaaagaa accgctgctg ctaaattcga
     5221 acgccagcac atggacagcg gaggtggagg tctcgagatg tggcccctga gggtgtacac
     5281 cagaaagaag agggagggcc agagactgaa cctgacaccc acacctgacc tgggctcccc
     5341 tgccaaggct gaagctcctc ccggcagcaa gaggaaaggc aaggtgcacg gcctgagcaa
     5401 gatcgctgag aaagccgaga gaagcaggca gggcgggtcc ggaagcggac ctttcagccc
     5461 caggctcgga gtgaccggcg agaagtccct gcaggagaac aggtccagcg aggacaccca
     5521 ggacgagaag attgcttccc tgagggagag cgtgaccgac gacctgcagg tggactccag
     5581 cagctccaac tccgaactgg tgtccggact gagcctgcat cacggcatgg cctcctccct
     5641 gctgtcctat agcgtgaccg attcctacgc cgagtacaag agcttcgagg agtccttccc
     5701 ctcccctgag ctgttcagga agtccgacta cctggactgg gagtgcccca atctggagga
     5761 gcacatgcag tggaagaaca gcaccctgct cgacacctcc aaggccgtgg ctatcgagaa
     5821 ggctccccag ttcagcaacg tcagcgccat cttctccacc agcagcgagg actaccagaa
     5881 gtgccacagg aagaccgtga tgaccgtggc cgatcagaac gtgtccccca aggccaagtg
     5941 cgccagcaat agcgagagcg acaacgctgc ctgtgaaatc ctgctggccg agaagacatg
     6001 ccccagcacc cccgagaaga ccaagaagaa gaagaccaac agcagcacac ctggcaagaa
     6061 gaacagagga ctgctgacct ccacacctag ctccgagacc gccggctttg tgatcgacct
     6121 gagctccgtg cagaaggcct cctttgagga gctgttcccc aacgtgagca actacgtgaa
     6181 cagcaacgag atcgtccccg tgtccagcct ccaggagaac agctccaacg agttccccgc
     6241 caacgcctcc gagatctgct gcatcatcag gacctcccct ggcaccaggc aggtgaaaaa
     6301 caagggcgtg attgtgaaga agaaaaagta cagcctgccc aaggacaccc cccaggacat
     6361 catcatcaag atggcctaag aattccttaa gtacgtaccg cgggctagcg gatccatgca
     6421 tacatgtgat atcactagtg attttaggga gagtttagta tccctggttc agaactccag
     6481 aaaactacag aacattagag acaatgtgga aaaggaatcg aaacgactga aaatatctta
     6541 acccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     6601 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6661 caattaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6841 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6901 gggctctatg gggtaccaca gacatgataa gatacattga tgagtttgga caaaccacaa
     6961 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     7021 taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag catacgcgtt
     7081 caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg
     7141 gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc
     7201 aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg
     7261 cccagaccct tgccctggtg gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc
     7321 tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa
     7381 ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc
     7441 tccggcgtgg tccagaccgc cactgcggcg ccgtcgtccg cgacccacac cttgccgatg
     7501 tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg
     7561 tccggatcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt
     7621 acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcata
     7681 tcgatagggc cgggattctc ctccacgtca ccgcatgtta gaagacttcc tctgccctct
     7741 ccgccctgtg ggaggaagag aagaggtcag gcgcgtgccc cactgtgggg tggaggggac
     7801 agataaaagt acccagaacc agagccacat taaccggccc tgggaatata aggtggtccc
     7861 agctcgggga cacaggatcc ctggaggcag caaacatgct gtcctgaagt ggacataggg
     7921 gcccgggttg gaggaagaag actagctgag ctctcggacc cctggaagat gccatgacag
     7981 ggggctggaa gagctagcac agactagaga ggtaaggggg gtaggggagc tgcccaaatg
     8041 aaaggagtga gaggtgaccc gaatccacag gagaacgggg tgtccaggca aagaaagcaa
     8101 gaggatggag aggtggctaa agccagggag acggggtact ttggggttgt ccagaaaaac
     8161 ggtgatgatg caggcctaca agaaggggag gcgggacgca agggagacat ccgtcggaga
     8221 aggccatcct aagaaacgag agatggcaca ggccccagaa ggagaaggaa aagggaaccc
     8281 agcgagtgaa gacggcatgg ggttgggtga gggaggagag atgcccggag aggacccaga
     8341 cacggggagg atccgctcag aggacatcac gtggtgcagc gccgagaagg aagtgctccg
     8401 gaaagagcat ccttgggcag caacacagca gagagcaagg ggaagaggga gtggaggaag
     8461 acggaacctg aaggaggcgg cagggaagga tctgggccag ccgtagaggt gacccaggcc
     8521 acaagctgca gacagaaagc ggcacaggcc caggggagag aatgctggtc agagaaagca
     8581 ggcgcg
//
