LOCUS       sequence-34340-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Sun Q, Westphal W, Wong KN, Tan I, Zhong Q
  TITLE     Rubicon controls endosome maturation as a Rab7 effector.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.
  PUBMED    20974968
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Nov 9. 107(45):19338-43."
COMMENT     Sequence Label: Flag-Rab7A Q86L
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..23
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     54..78
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             190..216
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             217..243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
       61 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      121 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
      181 ggatccatgt acccatacga tgttccggat tacgcttacc catacgatgt tccggattac
      241 gctggtacct ctaggaagaa agtgttgctg aaggttatca tcctgggaga ttctggagtc
      301 gggaagacat cactcatgaa ccagtatgtg aataagaaat tcagcaatca gtacaaagcc
      361 acaataggag ctgactttct gaccaaggag gtgatggtgg atgacaggct agtcacaatg
      421 cagatatggg acacagcagg actggaacgg ttccagtctc tcggtgtggc cttctacaga
      481 ggtgcagact gctgcgttct ggtatttgat gtgactgccc ccaacacatt caaaacccta
      541 gatagctgga gagatgagtt tctcatccag gccagtcccc gagatcctga aaacttccca
      601 tttgttgtgt tgggaaacaa gattgacctc gaaaacagac aagtggccac aaagcgggca
      661 caggcctggt gctacagcaa aaacaacatt ccctactttg agaccagtgc caaggaggcc
      721 atcaacgtgg agcangcgtt ccagacgatt gcacggaatg cacttaagca ggaaacgga
//
