LOCUS       sequence-343423-        9278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Sretenovic S, Liu S, Li G, Cheng Y, Fan T, Xu Y, Zhou J, Zheng X, 
            Coleman G, Zhang Y, Qi Y
  TITLE     Exploring C-To-G Base Editing in Rice, Tomato, and Poplar.
  JOURNAL   Front Genome Ed. 2021 Sep 15;3:756766. doi: 
            10.3389/fgeed.2021.756766. eCollection 2021.
  PUBMED    34713268
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2021-09-15"; volume: "3:756766. doi"; pages: " 
            10.3389/fgeed.2021.756766. eCollection 2021"
COMMENT     Sequence Label: pYPQ265L2
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2612..2632
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4085..4150
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4157..4177
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             8215..8223
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8303..8350
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    9025..9148
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9165..9184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9166..9184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9189..9205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9189..9205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgaa
     2581 aagaactgca gatggatccg agttcgagtc cccgaaaaag aaacggaaag tggcaaatga
     2641 gcttacatgg catgatgtgc tcgcggaaga aaagcagcag ccttatttcc tgaatacgct
     2701 tcagacggtt gcctctgaaa ggcaatctgg agttactata tatccgccac agaaggatgt
     2761 gtttaatgct ttcagattca cggaactggg cgacgtgaag gtggtgatct tgggacagga
     2821 cccctatcat ggcccgggcc aggcgcatgg cctcgccttt tctgttcggc cggggatcgc
     2881 catccctcca tctctcctga atatgtataa agaattggaa aatacaatcc cggggttcac
     2941 gcgcccgaac cacggttacc tggaatcatg ggccaggcag ggcgtgctgc tgctgaatac
     3001 ggtgctgacg gtccgggcgg gccaggcaca ttctcatgcc tcattgggtt gggagacgtt
     3061 tactgacaag gtgataagcc tgataaacca gcatcgggag ggagtcgttt tccttctttg
     3121 gggatctcat gcacagaaga agggagccat catagacaaa cagagacacc acgtcttgaa
     3181 ggcaccgcat ccctcacctc tctctgccca ccggggtttt tttgggtgta accacttcgt
     3241 cttggcaaat cagtggttgg aacagagagg ggaaactcct atcgattgga tgccggtcct
     3301 gccggcagaa tccgaatccg ggggttcggg gggaagtggc gggtcttcct ctgagacggg
     3361 acccgttgct gttgacccta ccctgaggcg cagaattgaa ccccacgaat tcgaagtctt
     3421 cttcgaccca cgggaacttg ctaaggaaac gtgtctgctg tatgagatca actggggagg
     3481 cagacattct atttggaggc acacaagcca aaatactaat aagcacgttg aggtgaactt
     3541 tatagaaaaa tttacaacag aaagatactt ttgtcctaac acaaggtgtt caataacttg
     3601 gttcctgagt tggtcaccct gcggcgaatg ttcgcgcgcg ataaccgaat tcctttcccg
     3661 ctacccacat gtcactctct ttatttatat tgcccggctc taccaccacg ccgatccgag
     3721 gaacagacag ggactccggg acctgatctc aagcggcgtt actatacaga ttatgacaga
     3781 gcaggaatcc ggttattgct ggcgcaactt tgttaattac tcgccctcaa atgaggcgca
     3841 ctggccacgg tacccacatt tgtgggtccg cttgtatgtg ttggagctgt attgcatcat
     3901 acttggcttg ccaccctgtc ttaacatact ccgccggaag cagcctcagc tcacgttttt
     3961 tacaatcgct ctgcaaagct gtcattatca gagacttccg ccacatatac tgtgggctac
     4021 tgggttgaag tcaggttctg agacccctgg cacaagtgag tcagcaacac ccgagtccac
     4081 catggattac aaggaccacg acggggatta caaggaccac gacattgatt acaaggatga
     4141 tgatgacaag atggctccga agaagaagag gaaggttggc atccacgggg tgccagctgc
     4201 tgacaagaag tactcgatcg gcctcgctat tgggactaac tctgttggct gggccgtgat
     4261 caccgacgag tacaaggtgc cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca
     4321 ttccatcaag aagaatctca ttggcgctct cctgttcgac agcggcgaga cggctgagag
     4381 gacgcggctc aagcgcaccg cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta
     4441 cctgcaggag attttctcca acgagatggc gaaggttgac gattctttct tccacaggct
     4501 ggaggagtca ttcctcgtgg aggaggataa gaagcacgag cggcatccaa tcttcggcaa
     4561 cattgtcgac gaggttgcct accacgagaa gtaccctacg atctaccatc tgcggaagaa
     4621 gctcgtggac tccacagata aggcggacct ccgcctgatc tacctcgctc tggcccacat
     4681 gattaagttc aggggccatt tcctgatcga gggggatctc aacccggaca atagcgatgt
     4741 tgacaagctg ttcatccagc tcgtgcagac gtacaaccag ctcttcgagg agaaccccat
     4801 taatgcgtca ggcgtcgacg cgaaggctat cctgtccgct aggctctcga agtctcggcg
     4861 cctcgagaac ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcgggaatct
     4921 cattgcgctc agcctggggc tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga
     4981 cgccaagctg cagctctcca aggacacata cgacgatgac ctggataacc tcctggccca
     5041 gatcggcgat cagtacgcgg acctgttcct cgctgccaag aatctgtcgg acgccatcct
     5101 cctgtctgat attctcaggg tgaacaccga gattacgaag gctccgctct cagcctccat
     5161 gatcaagcgc tacgacgagc accatcagga tctgaccctc ctgaaggcgc tggtcaggca
     5221 gcagctcccc gagaagtaca aggagatctt cttcgatcag tcgaagaacg gctacgctgg
     5281 gtacattgac ggcggggcct ctcaggagga gttctacaag ttcatcaagc cgattctgga
     5341 gaagatggac ggcacggagg agctgctggt gaagctcaat cgcgaggacc tcctgaggaa
     5401 gcagcggaca ttcgataacg gcagcatccc acaccagatt catctcgggg agctgcacgc
     5461 tatcctgagg aggcaggagg acttctaccc tttcctcaag gataaccgcg agaagatcga
     5521 gaagattctg actttcagga tcccgtacta cgtcggccca ctcgctaggg gcaactcccg
     5581 cttcgcttgg atgacccgca agtcagagga gacgatcacg ccgtggaact tcgaggaggt
     5641 ggtcgacaag ggcgctagcg ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa
     5701 cctgccaaat gagaaggtgc tccctaagca ctcgctcctg tacgagtact tcacagtcta
     5761 caacgagctg actaaggtga agtatgtgac cgagggcatg aggaagccgg ctttcctgtc
     5821 tggggagcag aagaaggcca tcgtggacct cctgttcaag accaaccgga aggtcacggt
     5881 taagcagctc aaggaggact acttcaagaa gattgagtgc ttcgattcgg tcgagatctc
     5941 tggcgttgag gaccgcttca acgcctccct ggggacctac cacgatctcc tgaagatcat
     6001 taaggataag gacttcctgg acaacgagga gaatgaggat atcctcgagg acattgtgct
     6061 gacactcact ctgttcgagg accgggagat gatcgaggag cgcctgaaga cttacgccca
     6121 tctcttcgat gacaaggtca tgaagcagct caagaggagg aggtacaccg gctgggggag
     6181 gctgagcagg aagctcatca acggcattcg ggacaagcag tccgggaaga cgatcctcga
     6241 cttcctgaag agcgatggct tcgcgaaccg caatttcatg cagctgattc acgatgacag
     6301 cctcacattc aaggaggata tccagaaggc tcaggtgagc ggccaggggg actcgctgca
     6361 cgagcatatc gcgaacctcg ctggctcgcc agctatcaag aaggggattc tgcagaccgt
     6421 gaaggttgtg gacgagctgg tgaaggtcat gggcaggcac aagcctgaga acatcgtcat
     6481 tgagatggcc cgggagaatc agaccacgca gaagggccag aagaactcac gcgagaggat
     6541 gaagaggatc gaggagggca ttaaggagct ggggtcccag atcctcaagg agcacccggt
     6601 ggagaacacg cagctgcaga atgagaagct ctacctgtac tacctccaga atggccgcga
     6661 tatgtatgtg gaccaggagc tggatattaa caggctcagc gattacgacg tcgatcatat
     6721 cgttccacag tcattcctga aggatgactc cattgacaac aaggtcctca ccaggtcgga
     6781 caagaaccgg ggcaagtctg ataatgttcc ttcagaggag gtcgttaaga agatgaagaa
     6841 ctactggcgc cagctcctga atgccaagct gatcacgcag cggaagttcg ataacctcac
     6901 aaaggctgag aggggcgggc tctctgagct ggacaaggcg ggcttcatca agaggcagct
     6961 ggtcgagaca cggcagatca ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac
     7021 taagtacgat gagaatgaca agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa
     7081 gctcgtctcc gacttcagga aggatttcca gttctacaag gttcgggaga tcaacaatta
     7141 ccaccatgcc catgacgcgt acctgaacgc ggtggtcggc acagctctga tcaagaagta
     7201 cccaaagctc gagagcgagt tcgtgtacgg ggactacaag gtttacgatg tgaggaagat
     7261 gatcgccaag tcggagcagg agattggcaa ggctaccgcc aagtacttct tctactctaa
     7321 cattatgaat ttcttcaaga cagagatcac tctggccaat ggcgagatcc ggaagcgccc
     7381 cctcatcgag acgaacggcg agacggggga gatcgtgtgg gacaagggca gggatttcgc
     7441 gaccgtcagg aaggttctct ccatgccaca agtgaatatc gtcaagaaga cagaggtcca
     7501 gactggcggg ttctctaagg agtcaattag gcctaagcgg aacagcgaca agctcatcgc
     7561 ccgcaagaag gactgggatc cgaagaagta cggcgggttc ctgtggccca ctgtggccta
     7621 ctcggtcctg gttgtggcga aggttgagaa gggcaagtcc aagaagctca agagcgtgaa
     7681 ggagctgctg gggatcacga ttatggagcg ctccagcttc gagaagaacc cgatcgattt
     7741 cctggaggcg aagggctaca aggaggtgaa gaaggacctg atcattaagc tccccaagta
     7801 ctcactcttc gagctggaga acggcaggaa gcggatgctg gcttccgcta agcagctgca
     7861 gaaggggaac gagctggctc tgccgtccaa gtatgtgaac ttcctctacc tggcctccca
     7921 ctacgagaag ctcaagggca gccccgagga caacgagcag aagcagctgt tcgtcgagca
     7981 gcacaagcat tacctcgacg agatcattga gcagatttcc gagttctcca agcgcgtgat
     8041 cctggccgac gcgaatctgg ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc
     8101 aatcagggag caggctgaga atatcattca tctcttcacc ctgacgaggc tcggcgcccc
     8161 tagggctttc aagtacttcg acacaactat cgatccgaag cagtacagga gcactaagga
     8221 ggtcctggac gcgaccctca tccaccagtc gattaccggc ctctacgaga cgcgcatcga
     8281 cctgtctcag ctcgggggcg acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa
     8341 gaagaagaag tgagctcaga gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag
     8401 ttcaatgcat cagtttcatt gcgcacacac cagaatccta ctgagtttga gtattatggc
     8461 attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat
     8521 tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat
     8581 ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt
     8641 gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg
     8701 tggcctctaa tgaccgaagt taatatgagg agtaaaacac ttgtagttgt accattatgc
     8761 ttattcacta ggcaacaaat atattttcag acctagaaaa gctgcaaatg ttactgaata
     8821 caagtatgtc ctcttgtgtt ttagacattt atgaactttc ctttatgtaa ttttccagaa
     8881 tccttgtcag attctaatca ttgctttata attatagtta tactcatgga tttgtagttg
     8941 agtatgaaaa tattttttaa tgcattttat gacttgccaa ttgttccgga actctagata
     9001 agcttaccgg aaagggcgaa ttcgcaactt tgtatacaaa agttgaacga gaaacgtaaa
     9061 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     9121 gtaaaacaca acatatccag tcactatgcc atccagctga tatcccctat agtgagtcgt
     9181 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     9241 acattgcaca agataaaaat atatcatcat gcctcctc
//
