LOCUS       sequence-343454-        6319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MYD88 knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pAPM-miR30-MYD88-ts3
FEATURES             Location/Qualifiers
     source          1..6319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(191..211)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        196..525
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      376..511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     438..457
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        537..916
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        918..1116
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1071..1091
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1134..1314
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1361..1486
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1983..2216
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2401..2445
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2743..2860
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2997..3404
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     ncRNA           4119..4133
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     LTR             4267..4447
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4518..5106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4598..4617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5277..6068,6069..6137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5900..5919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6138..6242)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(6317..6319,1..17))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
       61 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
      121 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
      181 tacgcgctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
      481 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cgcgttgaca
      541 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      601 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      661 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      721 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      781 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      841 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      901 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      961 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1021 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1081 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     1141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     1201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgagact
     1261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1321 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1381 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1441 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1501 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1561 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1621 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1681 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1741 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1801 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1861 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1921 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1981 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2041 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2101 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2161 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2221 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2281 agtaataaat ctctggaaca gatttggaat cacacgactt ggatggagtg ggacagagaa
     2341 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2401 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2461 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2521 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2581 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2641 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2701 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2761 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2821 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2881 cagagatcca gtttggttaa ttaactgcag ccccgataaa ataaaagatt ttatttagtc
     2941 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa
     3001 cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg
     3061 cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg
     3121 ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag
     3181 aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt
     3241 tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc
     3301 tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc
     3361 cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc cgggggtcta gaacgcgtgc
     3421 cgccatgacc gaatacaaac ctaccgtgag gctggctaca agagatgatg tcccaagggc
     3481 tgtgagaaca ctggccgccg cttttgccga ttaccctgcc acacgccaca ctgtggaccc
     3541 agatcggcat atcgagagag tgactgagct gcaggaactg ttcctgaccc gagtgggcct
     3601 ggacattggg aaggtctggg tcgcagacga tggagcagct gtggctgtct ggaccacacc
     3661 agagagcgtg gaagccggag ctgtctttgc agagatcggc cctagaatgg cagaactgag
     3721 cggctccagg ctggcagcac agcagcagat ggagggactg ctggccccac acaggcctaa
     3781 ggaaccagca tggttcctgg ctaccgtggg ggtctctcct gaccatcagg gcaaaggact
     3841 gggaagtgct gtggtcctgc caggagtgga ggctgcagaa cgagctggag tccctgcatt
     3901 tctggagacc tctgctccac gaaacctgcc cttctatgaa cggctgggct ttactgtgac
     3961 cgcagatgtg gaggtccccg aaggacctag gacctggtgc atgacacgca aacccggcgc
     4021 ctgagcgatc gccgcggccg ccttcttaac ccaacagaag gctcgagaag gtatattgct
     4081 gttgacagtg agcgaaacag acaaactatc gactgatagt gaagccacag atgtatcagt
     4141 cgatagtttg tctgttctgc ctactgcctc ggacttcaag gggctagaat tcggcagctg
     4201 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaact gcatccggac
     4261 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4321 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4381 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4441 tctagcaggg cccgtttcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4501 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4561 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4621 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4681 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4741 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4801 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4861 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4921 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4981 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5041 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5101 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5161 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5221 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5281 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5341 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5401 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5461 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5521 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5581 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5641 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5701 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5761 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5821 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5881 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5941 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6001 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6061 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6121 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6181 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6241 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     6301 tatggtgcac tctcagtac
//
