LOCUS       sequence-343456-        7901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for Candida albicans containing a red-shifted 
            firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Dorsaz S, Coste AT, Sanglard D
  TITLE     Red-Shifted Firefly Luciferase Optimized for Candida albicans In 
            vivo Bioluminescence Imaging.
  JOURNAL   Front Microbiol. 2017 Aug 3;8:1478. doi: 10.3389/fmicb.2017.01478. 
            eCollection 2017.
  PUBMED    28824601
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2017-08-3"; volume: "8:1478. doi"; pages: " 
            10.3389/fmicb.2017.01478. eCollection 2017"
COMMENT     Sequence Label: pDS1902
FEATURES             Location/Qualifiers
     source          1..7901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(350..454)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      481..936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(568..587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     778..799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1062..1084
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1076..1093
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1077..1093
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1100..1119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1100..1118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2534..2550
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2534..2550
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(6190..6208)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6229..6245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6229..6245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6242..6264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6253..6269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6277..6283,6284..6301,6302..6307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6322..6343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6460..6477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6631..7219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6711..6730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7390..7901,1..280,281..349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
       61 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
      121 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
      181 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
      241 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
      301 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
      361 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      421 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
      481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      901 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
      961 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     1021 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     1081 aacgacggcc agtgaattgt aatacgactc actatagggc gaattggagc tccaccgcgg
     1141 tggcggccgc ggccgacttg gccaggccta gatcccgact aataggaatt gatttggatg
     1201 gtataaacgg aaacaaaaaa aagagctggt actactttct ttaaaattat tttattattt
     1261 gattttattt aatagtatat attatatttt gaacgtagat tattttgttg aaagttgctg
     1321 tagtgccatt gattcgtaac actaattctg tattagtcat tcctcttgtt tgatagtatc
     1381 caaaaaaacg gctatttttt tgcaatctta tttcctgcat attatacaga taacataatg
     1441 aaagaaaaaa tctttttttt tgttcttcaa tgatgatttc aaccattctt ttaaacattg
     1501 atcaattcct gagcaacaac cccatacaca ctggtttata tatcgcccct tttacagttg
     1561 aagaaagaaa tagaaataga aatagcaaac aaaagatatg acagtcaaca ctaagaccta
     1621 tagtgagaga gcagaaactc atgcctcacc agtagcacaa cgattatttc gattaatgga
     1681 actgaagaaa accaatttat gtgcatcaat tgatgttgat accactaagg aattccttga
     1741 attaattgat aaattgggtc cttatgtatg cttaatcaag actcatattg atataatcaa
     1801 tgatttttcc tatgaatcca ctattgaacc attattagaa ctttcacgta aacatcaatt
     1861 tatgattttt gaagatagaa aatttgctga tattggtaat accgtgaaga aacaatatat
     1921 tggtggagtt tataaaatta gtagttgggc agatattact aatgctcatg gtgtcactgg
     1981 gaatggagta gttgaaggat taaaacaggg agctaaagaa accaccacca accaagagcc
     2041 aagagggtta ttgatgttag ctgaattatc atcagtggga tcattagcat atggagaata
     2101 ttctcaaaaa actgttgaaa ttgctaaatc cgataaggaa tttgttattg gatttattgc
     2161 ccaacgtgat atgggtggac aagaagaagg atttgattgg cttattatga cacctggagt
     2221 tggattagat gataaaggtg atggattagg acaacaatat agaactgttg atgaagttgt
     2281 tagcactgga actgatatta tcattgttgg tagaggattg tttggtaaag gaagagatcc
     2341 agatattgaa ggtaaaaggt atagagatgc tggttggaat gcttatttga aaaaaactgg
     2401 ccaattataa atgtgaaggg ggagattttc actttattag atttgtatat atgtataata
     2461 aataaataaa taagttaaat aaataattaa ataagggtgg taattattac tatttacaat
     2521 caaaggtggt ccttctagaa ctagtggatc ccccagatcc ttatccttaa acagattcta
     2581 aaacaacatc tttgaaacca gaagaaactt tcttaccttt ttcttcggtt gaaccttcac
     2641 cgtgcaaagc caataatgaa cccaagtcga attttggttg tttcaacaat ttgacttttc
     2701 tgatgtggac attttgtaat gggaaaatgg tttgggtgga tttttcaatt tcacggccaa
     2761 tgacttctgg aatcaatttg gaagttaatt gagctaaagt acagttggaa acttctcttt
     2821 gcataatttc aatcatcttc tttctgactt ctctcaattt agaggattga gcgtaagtag
     2881 ttttcttgat ttggtttggt tgtcttttgg tgaaagcaat ggcaaaaact ctcaaaacgt
     2941 aatcgtcgga agttttaaca gtgacattag cttcaactaa tgattgccat tttctgacca
     3001 atgatcttaa tttgtcagaa gtgaaatcca aaccatgagg cctccatgag gcctccatga
     3061 ggcctccatg aggcctccat ggaggcctca tggaagttgg tcaacaagtt tttaccttgg
     3121 acttcatcaa ctctcaattt gatttttctg taagagtggt cttcggaacc ttgtaagtcg
     3181 gccaaacaaa cttcgaaaac tctacctttc aagccatcag cggcattctt taaaccggta
     3241 gatctgttga tcaaagtttt accaacattt ctgttttcaa aagtggttgg agctttgatg
     3301 tcaaaccaat cttttctggt gaatgggtca acgaccttct tttttaatcc tttctttcct
     3361 ttggacaatc tcttgttttt accgacagcc atgttgtact tgagttggat ctacgcgaat
     3421 ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa tagggacgct
     3481 agcctataca gcaattttac cacctttctt agctttgatc aaaatttctc tgatctttct
     3541 agcatccaat ttaccagtca aaccttttgg aacttcatca acgaaaacaa caccacctct
     3601 caatttctta gcagtggtaa cttgagaagc aacgtaatca acaatttctt tttcagtcat
     3661 ggttttaccg tgttccaaaa caacaacagc agctggcaat tcaccagcat catcatctgg
     3721 caaaccagca acaccagcat caaaaatgtt tgggtgttgc aacaagatag attccaattc
     3781 agctggagca acttggtaac ctttgtattt gatcaaagat ttcaatctgt caacaatgaa
     3841 gaagtgttca tcttcatccc agtaagcaat atcaccagag tgtaaccaac catctttatc
     3901 aatcaaagcg ttggtagctt ctgggttgtt aacgtaacca gacatgatca ttggacctct
     3961 aacacacaat tcacctcttt ggttaacacc caaagtttta ccagtatcca aatcaacaac
     4021 tttagcttca aagaatggaa caactttacc aacagcacct ggtttgtcat caccttctgg
     4081 agtgatcaaa atagcagaag tggtttcagt caaaccgtaa ccttgtctaa tacctggcaa
     4141 gtggaatctt ttagcaacag cttcaccaac ttctttagac aatggagcac caccagaagc
     4201 aatttcgtgc aagttagaca aatcgtattt atcaatcaaa gtagatttag ccaagaaaga
     4261 gaacaaagtt ggaaccaaca aagcagtttg aatcttgtaa tcttgcaaag atctcaagaa
     4321 caattcttct tcgaatctgt acatcaaaac aactctgaaa ccacaaatca agtaacccaa
     4381 agtagtgaac ataccgaaac cgtggtggaa tggaacaaca gacaaaatag cagtatctgg
     4441 aatgatttgg ttaccgaaaa ttggatctct agcgtgagag aatctaacac acaaagctct
     4501 gtgtggcaaa gcaacacctt ttggcaaacc agtagaacca gaagagttca taatcaaagc
     4561 aatggttttg tctctgtcaa aagattctgg aacgaaatcg tattcgttga aacctggtgg
     4621 caagtgagaa gtaacgaaag tgtacataga ttggaaacct tggtaatcag ttttagaatc
     4681 catgatgatg atcttttgaa taattggcaa tttcttttga acgttcaaga tcttttgcaa
     4741 acctttctta gaaacgaaaa caacagttgg ttgagaaata cccatggagt tcaacaattc
     4801 tctttcgttg taaatatcgt tagctggagc aacagcaaca ccaatgaaca aagcacccaa
     4861 aactggcatg aagaattgca aagagttttc actacaaaca acaattctgt ggttggtgtt
     4921 caaaccgtat cttttcatag cttcagccaa tctgacagac atttcaaagt attcagcgta
     4981 agtgatatca acttcaatgt gagcatcagt gaaagcaata gtacctggaa ccaaagcgta
     5041 tcttttcata gctttgtgca attgttcacc agcagtacca tcttccaatg ggtagaatgg
     5101 agctggacct ttcttgatgt tcttagcatc ttccatttta agctttttga atgattatat
     5161 ttttttaata ttaatatcga gataatgttt ctaagaaaga aagaaaacca ggagtgaaaa
     5221 ttagaaaagg aaaggaaagg aaaaaaagaa aaatctgaaa atatataaaa aaaaattgtt
     5281 tcgttggcaa taaatcttgg tgagaacagc gaccgaaagc aaataagaac aaaatatgag
     5341 tgtattacgt tgaacaacta attaacgtgt gtgtatggat ctttttttct tttttctctt
     5401 taaccgacta taaacaacaa acatttttgg gcagtgcaca cactacttaa tatacacagc
     5461 ataaattaca cgattagaaa caaattagct tattaaaata acctaatcaa accgaatatt
     5521 ttatggtatt atgagtaaac tatataatat aaatagcaca cacccacaac aacaacaaag
     5581 gaaaactaaa aggttttttc tttttgaaaa gatcgttttc tttattattc tctagttttg
     5641 acgctgaaat attcgttgta ctgttgcaaa tttgtatttg gtctgcctta tcttcttttc
     5701 tacacttttt acttatgagc tttataattc acagataagt ttcaggtttg ccagatatac
     5761 ttgcttcact ggttactaaa ctctcggtgt acaaatgact tgcttatgta agaggctact
     5821 tttctagttt tgcagtcatt catttaaaaa caattgatcc tgaaggctct aaagacatag
     5881 taggtttact ttgactgtat atttccgtgg catgtttaaa ggccccttgg ccataggata
     5941 ttggtggcgg cacagtaagc attaaaattg ttgtttcgcg gaattacaac atttcttctt
     6001 ttacataata atgtcactcc tttttgattg gcatagccga gatctttttt taacacagac
     6061 tttaagctgt tttttgccac ctgagaacag tttctatttt ggtccaaaag aaatattaga
     6121 ctaagtttat tataaacgag gctggtgatc ttaatagctc gagggggggc ccggtaccag
     6181 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     6241 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6301 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6361 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6421 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6481 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6541 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6601 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6661 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6721 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6781 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6841 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6901 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6961 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7021 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7081 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7141 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7201 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7261 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7321 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7381 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7441 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7501 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7561 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7621 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7681 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7741 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7801 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7861 gttatcactc atggttatgg cagcactgca taattctctt a
//
