LOCUS       sequence-343461-        6319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LRPPRC knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pAPM-miR30-LRPPRC-ts1
FEATURES             Location/Qualifiers
     source          1..6319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     270..290
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     464..483
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        558..662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(663..731,732..1523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(881..900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1694..2282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2183..2202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             2353..2533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     ncRNA           complement(2667..2681)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(3396..3803)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3940..4057
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4355..4399)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4584..4817
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5314..5439
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5486..5666
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5684..5882)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5709..5729)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5884..6263
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(join(6275..6319,1..285))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(6289..6319,1..105)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
       61 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      121 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
      181 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
      241 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagcgcgta
      301 tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg
      361 caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg
      421 acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt
      481 gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca
      541 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      601 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      661 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      721 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      781 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      841 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      901 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      961 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1021 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1081 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1141 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1201 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1261 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1321 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1381 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1441 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1501 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1561 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1621 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1681 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1741 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1801 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1861 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1921 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1981 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2041 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2101 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2161 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2221 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2281 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2341 tgaaacgggc cctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     2401 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     2461 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     2521 accagagaga cccagtacag tccggatgca gttagccctt ccagtccccc cttttctttt
     2581 aaaaagtggc taagatctac agctgccgaa ttctagcccc ttgaagtccg aggcagtagg
     2641 caacagcaat ctcattaaat tacatacatc tgtggcttca ctatgtaatt taatgagatt
     2701 gctggcgctc actgtcaaca gcaatatacc ttctcgagcc ttctgttggg ttaagaaggc
     2761 ggccgcggcg atcgctcagg cgccgggttt gcgtgtcatg caccaggtcc taggtccttc
     2821 ggggacctcc acatctgcgg tcacagtaaa gcccagccgt tcatagaagg gcaggtttcg
     2881 tggagcagag gtctccagaa atgcagggac tccagctcgt tctgcagcct ccactcctgg
     2941 caggaccaca gcacttccca gtcctttgcc ctgatggtca ggagagaccc ccacggtagc
     3001 caggaaccat gctggttcct taggcctgtg tggggccagc agtccctcca tctgctgctg
     3061 tgctgccagc ctggagccgc tcagttctgc cattctaggg ccgatctctg caaagacagc
     3121 tccggcttcc acgctctctg gtgtggtcca gacagccaca gctgctccat cgtctgcgac
     3181 ccagaccttc ccaatgtcca ggcccactcg ggtcaggaac agttcctgca gctcagtcac
     3241 tctctcgata tgccgatctg ggtccacagt gtggcgtgtg gcagggtaat cggcaaaagc
     3301 ggcggccagt gttctcacag cccttgggac atcatctctt gtagccagcc tcacggtagg
     3361 tttgtattcg gtcatggcgg cacgcgttct agacccccgg gcgactcagt ctgtcggagg
     3421 actggcgcgc cgagtgaggg gttgtgagct cttttataga gctcgggaag cagaagcgcg
     3481 cgaacagaag cgagaagcag gctgattggt taattcaaat aaggcgcagg gtcatttcag
     3541 gtccttgggg gagcctggaa acatctgatg ggtcttaaga aactgctgag ggttgggcca
     3601 tatctgggga ccatctgttc ttggcctcgg gccggggccg aaactgcggt gaccatctgt
     3661 tcttggcccc gggccggggc cgaaactgct caccgcagat atcctgtttg gcccaacgtt
     3721 agctattttc atgtacccgc ccttgatctg aacttctcta ttcttggttt ggtatttttc
     3781 catgccttgc aaaatggcgt tactgcagct agcttgccaa acctacaggt ggggtctttc
     3841 attcccccct ttttctggag actaaataaa atcttttatt ttatcggggc tgcagttaat
     3901 taaccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
     3961 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     4021 tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg
     4081 tctgcagaat tggcgcacgc agtgccgatc cgttcactaa tcgaatggat ctgtctctgt
     4141 ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga
     4201 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
     4261 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
     4321 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
     4381 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
     4441 agcttgtgta attgttaatt tctctgtccc actccatcca agtcgtgtga ttccaaatct
     4501 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
     4561 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
     4621 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
     4681 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
     4741 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
     4801 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
     4861 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
     4921 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cggccgcttg
     4981 ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     5041 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     5101 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     5161 gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc
     5221 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     5281 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     5341 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     5401 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     5461 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     5521 agggatctct agttaccaga gtctcacaac agacgggcac acactacttg aagcactcaa
     5581 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     5641 cagatctggt ctaaccagag agacccagta caggcaaaac gcgctgctta tatagacctc
     5701 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     5761 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     5821 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     5881 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     5941 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     6001 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     6061 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     6121 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     6181 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     6241 ataactagtc aataatcaat gtcaacgcga gctttttgca aaagcctagg cctccaaaaa
     6301 agcctcctca ctacttctg
//
