LOCUS       sequence-343469-        3145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TESEC expression vector for DapB from M. tuberculosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Bongaerts N, Edoo Z, Abukar AA, Song X, Sosa-Carrillo S, 
            Haggenmueller S, Savigny J, Gontier S, Lindner AB, Wintermute EH
  TITLE     Low-cost anti-mycobacterial drug discovery using engineered E. coli.
  JOURNAL   Nat Commun. 2022 Jul 7;13(1):3905. doi: 10.1038/s41467-022-31570-3.
  PUBMED    35798732
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-7"; volume: "13(1):3905. doi"; pages: " 
            10.1038/s41467-022-31570-3"
COMMENT     Sequence Label: pNB207
FEATURES             Location/Qualifiers
     source          1..3145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..303
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     226..245
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             314..325
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1104..1190
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1355..1943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1435..1454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2031..2125)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2155..2946,2947..3015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2778..2797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3016..3120)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaag acatggagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
       61 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
      121 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
      181 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
      241 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
      301 catagaattc attaaagagg agaaaggtac catgcgcgtc ggcgttttag gcgcgaaggg
      361 taaggtaggt gcgacaatgg ttcgtgctgt agcagcggcc gatgacttga cgttgtccgc
      421 ggaactcgat gcgggcgatc cattatctct gttgaccgat gggaacacgg aagttgtcat
      481 tgattttacc catcctgatg tcgtgatggg caatctggaa tttttgatcg acaacggtat
      541 tcatgcggtt gtaggcacga cgggcttcac ggcagaacgc ttccagcagg tcgagtcgtg
      601 gctggttgca aaacccaaca ctagcgtgct gattgcgccg aattttgcga ttggtgctgt
      661 tctgtctatg cactttgcga aacaggctgc gcgctttttc gattcagccg aagttattga
      721 actccaccac ccacataaag ccgatgcgcc tagcggcacc gcagctcgta cggcgaaact
      781 gattgcggaa gcccgcaaag gactgccacc taacccggat gccaccagta cgtctttacc
      841 gggcgcgcgc ggcgctgatg ttgacgggat cccagtgcat gcggttcgcc tggcgggcct
      901 ggttgctcat caggaggtgt tgtttgggac cgagggcgaa accctgacaa ttcgtcacga
      961 cagcttagac cgcacgtcat tcgtcccggg ggtactcctg gctgtgcgtc gtatcgcaga
     1021 gcgtccgggt ttgactgtgg gactggaacc gttattggat ctgcactgaa ggtgggatcc
     1081 catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1141 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
     1201 agacctaggg cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1981 agtggaacga aaactcacgt taagggattt tggtcatgac tagtgcttgg attctcacca
     2041 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     2101 attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagttacca
     2161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2281 tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     2461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     2521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     2581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3121 cacatttccc cgaaaagtgc cacct
//
