LOCUS       sequence-343471-        6319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DDX17 knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pAPM-miR30-DDX17-ts1
FEATURES             Location/Qualifiers
     source          1..6319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    113..238
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    735..968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1153..1197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1495..1612
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1749..2156
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     ncRNA           2871..2885
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     LTR             3019..3199
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3270..3858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3350..3369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4029..4820,4821..4889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4652..4671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4890..4994)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5069..5088)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5262..5282)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5267..5596
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5447..5582
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5509..5528
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        5608..5987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5989..6187
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6142..6162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             join(6205..6319,1..66)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgttgtgaga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
       61 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      121 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      181 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      241 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      301 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      361 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
      421 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
      481 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
      541 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
      601 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
      661 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
      721 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
      781 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
      841 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
      901 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
      961 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1021 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ttggatggag
     1081 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     1141 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     1201 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     1261 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     1321 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     1381 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     1441 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     1501 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     1561 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     1621 ttacagggac agcagagatc cagtttggtt aattaactgc agccccgata aaataaaaga
     1681 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     1741 tagctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga atagagaagt
     1801 tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga tatctgcggt
     1861 gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt tcggccccgg
     1921 cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt ttcttaagac
     1981 ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct tatttgaatt
     2041 aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag ctctataaaa
     2101 gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccgggggtc
     2161 tagaacgcgt gccgccatga ccgaatacaa acctaccgtg aggctggcta caagagatga
     2221 tgtcccaagg gctgtgagaa cactggccgc cgcttttgcc gattaccctg ccacacgcca
     2281 cactgtggac ccagatcggc atatcgagag agtgactgag ctgcaggaac tgttcctgac
     2341 ccgagtgggc ctggacattg ggaaggtctg ggtcgcagac gatggagcag ctgtggctgt
     2401 ctggaccaca ccagagagcg tggaagccgg agctgtcttt gcagagatcg gccctagaat
     2461 ggcagaactg agcggctcca ggctggcagc acagcagcag atggagggac tgctggcccc
     2521 acacaggcct aaggaaccag catggttcct ggctaccgtg ggggtctctc ctgaccatca
     2581 gggcaaagga ctgggaagtg ctgtggtcct gccaggagtg gaggctgcag aacgagctgg
     2641 agtccctgca tttctggaga cctctgctcc acgaaacctg cccttctatg aacggctggg
     2701 ctttactgtg accgcagatg tggaggtccc cgaaggacct aggacctggt gcatgacacg
     2761 caaacccggc gcctgagcga tcgccgcggc cgccttctta acccaacaga aggctcgaga
     2821 aggtatattg ctgttgacag tgagcgcagc cccgattctc tgtgtttata gtgaagccac
     2881 agatgtataa acacagagaa tcggggctat gcctactgcc tcggacttca aggggctaga
     2941 attcggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3001 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3181 gtgtggaaaa tctctagcag ggcccgtttc atgtgagcaa aaggccagca aaaggccagg
     3241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     5041 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5101 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     5161 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     5221 ttgcgctgct tcgcgatgta cgggccagat atacgcgctg tggaatgtgt gtcagttagg
     5281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5341 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5401 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5521 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5581 cctaggcttt tgcaaaaagc tcgcgttgac attgattatt gactagttat taatagtaat
     5641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     5701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     5881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     5941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6181 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     6241 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6301 caagtagtgt gtgcccgtc
//
