LOCUS       sequence-343476-        3320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pWSK29 with Vibrio cholerae wzzO139 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Murray GL, Attridge SR, Morona R
  TITLE     Altering the length of the lipopolysaccharide O antigen has an 
            impact on the interaction of Salmonella enterica serovar Typhimurium
            with macrophages and complement.
  JOURNAL   J Bacteriol. 2006 Apr;188(7):2735-9. doi: 
            10.1128/JB.188.7.2735-2739.2006.
  PUBMED    16547065
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.188.7.2735-2739"; journalName: "J Bacteriol"; date: 
            "2006-04"; volume: "188"; issue: "7"; pages: "2735-9"
COMMENT     Sequence Label: pWSK29-wzzO139
FEATURES             Location/Qualifiers
     source          1..3320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     86..103
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    118..257
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(183..202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     343..365
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(359..550)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1059..1078
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1059..1077
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1109..1131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1139..1171
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     1216..1232
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1216..1232
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1336..1354)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1350..1397
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1528..1632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1633..1701,1702..2493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1851..1870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2664..3252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3153..3172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
       61 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
      121 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc
      181 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
      241 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      301 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      361 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
      421 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
      481 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
      541 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
      601 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
      661 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
      721 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
      781 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
      841 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
      901 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
      961 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     1021 aggacccaac gctgcccgag atctcgatcc cgcgaaatta atacgactca ctatagggag
     1081 accacaacgg tttccctcta gaaataattt tgtttaactt taagaaggag atatacatat
     1141 ggctagcatg actggtggac agcaaatggg tcgggattca agcttggtac cgagctccac
     1201 cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt ctgcagatat
     1261 ccatcacact ggcggccgct cgagcagatc cggctgctaa caaagcccga aaggaagctg
     1321 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     1381 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg ataattcttg aagacgaaag
     1441 ggcctcgtgg tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     1501 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1561 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1621 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1681 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1741 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1801 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1861 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1921 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1981 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2041 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2101 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2161 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2221 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2281 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2341 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2401 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2461 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2521 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2581 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2641 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2701 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2761 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     2821 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2881 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2941 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3001 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3061 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3121 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3181 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3241 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3301 tttgctcaca tgttctttcc
//
