LOCUS       sequence-343477-        8623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic FUCCI construct driven by a CAG promoter. Contains 
            I-SceI sites for transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Duerr TJ, Jeon EK, Wells KM, Villanueva A, Seifert AW, McCusker CD, 
            Monaghan JR
  TITLE     A constitutively expressed fluorescent ubiquitination-based 
            cell-cycle indicator (FUCCI) in axolotls for studying tissue 
            regeneration.
  JOURNAL   Development. 2022 Mar 15;149(6). pii: 274737. doi: 
            10.1242/dev.199637. Epub 2022 Mar 17.
  PUBMED    35266986
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2022-03-15"; volume: "149(6). pii: 274737. 
            doi"; pages: " 10.1242/dev.199637. Epub 2022 Mar 17"
COMMENT     Sequence Label: CAG FUCCI
FEATURES             Location/Qualifiers
     source          1..8623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      90..678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     579..598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     832..849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    966..987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1002..1007,1008..1025,1026..1032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1040..1056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1064..1080
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1124..1142
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1179..1195
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1229..1245)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1314..1330
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1350..1370
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1372..1390
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1416..1432
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        1523..1826
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1828..2106
                     /label=chicken beta-actin promoter
     intron          2107..3123
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3047..3069
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     complement(3200..3216)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3253..3271)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3271..3295
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          3326..3898
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3996..4005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4002..4679
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             4002..4679
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     protein_bind    complement(5011..5035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5046..5099
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5100..5807
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5245..5263)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    6419..6553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6443..6462)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6497..6516
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(6724..6744)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6752..6768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6887..6905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6938..6954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7096..7551
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7183..7202)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7393..7414
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7577..7681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7682..7750,7751..8542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7900..7919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
       61 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      721 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      781 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      841 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      901 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      961 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     1021 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     1081 catgattacg ccaagcgcgt agggataaca gggtaatgcg cgcaattaac cctcactaaa
     1141 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg
     1201 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagcgg ccgccagtgt
     1261 gatggatatc tgcagaattc gcccttctga taccagcgga taacaatttc acacaggaaa
     1321 cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg gtaatacgac
     1381 tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt
     1441 gatatcgaat tcctgcagcc cctagttatt aatagtaatc aattacgggg tcattagttc
     1501 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     1561 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     1621 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     1681 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     1741 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     1801 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     1861 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     1921 cagcgatggg ggcggggggg gggggggggg cgcgcgccgg ggggggsggg gsggggsgrg
     1981 gggsggggsg gggsgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
     2041 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     2101 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     2161 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     2221 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     2281 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     2341 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     2401 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     2461 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     2521 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     2581 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     2641 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     2701 ggggcggggc cgcctcgggc cggggrgggc tcgggggagg ggcgcggcgg cccccggagc
     2761 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     2821 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     2881 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     2941 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     3001 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     3061 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     3121 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattattc
     3181 cgctagcgct accggggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc
     3241 cagcttttgt tccctttagt gagggttaat tcaagtttgt acaaaaaagc aggcttcacc
     3301 gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt
     3361 cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat
     3421 gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct
     3481 actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt
     3541 aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg
     3601 taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc
     3661 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
     3721 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc tggtcatcat
     3781 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
     3841 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt tcctacagct
     3901 cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tcctcgacgg
     3961 atccactagt aacggccgcc agtgtgctgg aattcgccac catggtgagc gtgatcaagc
     4021 ccgagatgaa gatcaagctg tgcatgaggg gcaccgtgaa cggccacaac ttcgtgatcg
     4081 agggcgaggg caagggcaac ccctacgagg gcacccagat cctggacctg aacgtgaccg
     4141 agggcgcccc cctgcccttc gcctacgaca tcctgaccac cgtgttccag tacggcaaca
     4201 gggccttcac caagtacccc gccgacatcc aggactactt caagcagacc ttccccgagg
     4261 gctaccactg ggagaggagc atgacctacg aggaccaggg catctgcacc gccaccagca
     4321 acatcagcat gaggggcgac tgcttcttct acgacatcag gttcgacggc accaacttcc
     4381 cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc accgagaaga
     4441 tgtacgtgga ggacggcgtg ctgaagggcg acgtgaacat gaggctgctg ctggagggcg
     4501 gcggccacta caggtgcgac ttcaagacca cctacaaggc caagaaggag gtgaggctgc
     4561 ccgacgccca caagatcgac cacaggatcg agatcctgaa gcacgacaag gactacaaca
     4621 aggtgaagct gtacgagaac gccgtggcca ggtactccat gctgcccagc caggccaagg
     4681 gatatccatc acactggcgg ccgctcgaga tgagttccat cagaagacca aagaatgcag
     4741 aaaacccgtc tgaaaacatc aagaaattcc tggtagcgcc aacatctgga gcaggaatgg
     4801 ggaggaggac gcttcaggtg cttcagccct ctgctgtaaa caaaaacctt ggtcgtataa
     4861 ttgagaatgg taaagcagtg gccaaaagga agatgtggag cgccgagcaa gttaagggtt
     4921 caaagagagt caaagcagag gtggctgtca aatccacaaa tgcagaaaat gaaaaccagc
     4981 ctgagggggt cacacaagag gcctatgagc acccagcttt cttgtacaaa gtggttggca
     5041 gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccaa
     5101 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     5161 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     5221 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     5281 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     5341 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     5401 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     5461 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     5521 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     5581 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     5641 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     5701 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     5761 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga tatccatcac
     5821 actggcggcc gctcgagatg gctcaagctc gtgttactga ttattttgca cagagtaaaa
     5881 aagctggtgt ttctcgatct ttacggtcta agggacaaaa ggtgtccggg gatgtagtag
     5941 aatcagcggt gatcaataaa ccgaggagtt cgagcagagc gtccggttct tcacgcaaag
     6001 cgacgcacag tgagatcacc acagcagagc cgcagaaaca aactcagctg gagtttctca
     6061 aagtcatcga cgaggcttta tcgacggaga ctgcagatac cgtcgcggat tcgcgtgatg
     6121 tgaagattga gggcttaacg gcgagtccca ggacccctaa gcggagttca ccggagttcg
     6181 acgtgtgttc ggtgttgttt ccgtccacag ccgagctgca tagcagcgcc aagaagcggc
     6241 agagactcaa cgcgggccac aactgcagga gctcaccgga ggagcgcgct ggacagaaaa
     6301 ctgcgaggaa aaaactctat ctactggcaa gcgacgacaa agtaaagacc actgagccct
     6361 tggccagcag cagccctcag gcacctcaac aaactgcccg caaagagtaa tcgatgatga
     6421 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6481 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6541 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6601 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     6661 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     6721 actcaacttt attatacata gttgataatt cactggccgt cgttttacaa cgtcgtgact
     6781 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acaaagggcg aattccagca
     6841 cactggcggc cgttactagt ggatccgagc tcggtaccca attcgcccta tagtgagtcg
     6901 tattacgcgc gcattaccct gttatcccta cgcgctcact ggccgtcgtt ttacaacgtc
     6961 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7021 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7081 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7141 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7201 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7261 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7321 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7381 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7501 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7561 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8041 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atc
//
