LOCUS       sequence-34348-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-Express SMR5448
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     360..378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      301 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
      361 ataggcgtat cacgaggccc tggcgcgcct aggccatatg ggcccgggcg agcatattga
      421 tccgctgcaa actgaaattg gcagcatggt ctatgcggtg aaaccaggcg atggttctgc
      481 gcgtgaacag gcggcgagct gccagcgtgt gattggcggt ctggcgaata ttgccgagga
      541 gtacgcgact aaacgctatc gttctaacgt catcaactgg gggatgttac cgctgcaaat
      601 ggcggaagta ccagcctttg aagtggggga ttacatttac atccctggca ttaaagcggc
      661 gctggataat ccgggtacga cgtttaaagg ttatgtgatc catgaagatg cgccggtaac
      721 ggaaattacg ctctatatgg aaagtctgac tgctgaagag cgcgagatta tcaaggcggg
      781 tagtttgatt aacttcaata aaaaccgtca gatgtaaaaa gcgccatgtg aatgtaggtc
      841 gcattcggca cttattgtcg gatgcgatgc ttgcgcatct tatccgacct acgaatcgca
      901 tcgaatctgt agccagata
//
