LOCUS       sequence-343499-       10630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PYL1-sfGFP-HP1Adel72.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Gao Y, Han M, Shang S, Wang H, Qi LS
  TITLE     Interrogation of the dynamic properties of higher-order 
            heterochromatin using CRISPR-dCas9.
  JOURNAL   Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034.
  PUBMED    34428454
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034."
COMMENT     Sequence Label: pHR: pPGK PYL1-sfGFP-HP1Adel72
FEATURES             Location/Qualifiers
     source          1..10630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             749..1459
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             749..1459
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(776..804)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1399..1419
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    1878..2466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1931..1951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2349..2360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2469..2485)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2470..2486)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2576..2809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2834..2852)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2835..2852)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3142..3161)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3375..3394)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3494..3516
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3554..3572)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3640..3744
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3745..3813,3814..4605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3963..3982)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4776..5364
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5265..5284
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5518..5535
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5605..5625)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5610..5939
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5790..5925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7073..7138
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7268..7288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7713..7847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7750..7769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7804..7823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8015..8648
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8695..8820
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9317..9550
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9735..9779
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10064..10181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10246..10630,1..115)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10281..10304)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
       61 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
      121 cagcccaagc ttacgcgtct gcaggatatc aagcttgcgg tacgactgag tcatgggtgg
      181 gggcgcgcca actcaagacg aattcaccca actctcccaa tcaatcgccg agttccacac
      241 gtaccaactc ggtaacggcc gttgctcatc tctcctagct cagcgaatcc acgcgccgcc
      301 ggaaacagta tggtccgtgg tgagacgttt cgataggcca cagatttaca aacacttcat
      361 caaaagctgt aacgtgagtg aagatttcga gatgcgagtg ggatgcacgc gcgacgtgaa
      421 cgtgataagt ggattaccgg cgaatacgtc tcgagagaga ttagatctgt tggacgatga
      481 tcggagagtg actgggttta gtataaccgg tggtgaacat aggctgagga attataaatc
      541 ggttacgacg gttcatagat ttgagaaaga agaagaagaa gaaaggatct ggaccgttgt
      601 tttggaatct tatgttgttg atgtaccgga aggtaattcg gaggaagata cgagattgtt
      661 tgctgatacg gttattagat tgaatcttca gaaacttgct tcgatcactg aagctatgaa
      721 cgtggatcca ggtggaggtg gaagcggtag caaaggagaa gaacttttca ctggagttgt
      781 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tccgtggaga
      841 gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca ctactggaaa
      901 actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc
      961 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     1021 tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt
     1081 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga
     1141 agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca atgtatacat
     1201 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacgttga
     1261 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     1321 tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga aagatcccaa
     1381 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
     1441 catggatgag ctctacaaag gtggaggtcg gaccggatca atgaaggagg gtgaaaataa
     1501 taaacccagg gagaagtcag aaagtaacaa gaggaaatcc aatttctcaa acagtgccga
     1561 tgacatcaaa tctaaaaaaa agagagagca gagcaatgat atcgctcggg gctttgagag
     1621 aggactggaa ccagaaaaga tcattggggc aacagattcc tgtggtgatt taatgttcct
     1681 aatgaaatgg aaagacacag atgaagctga cctggttctt gcaaaagaag ctaatgtgaa
     1741 atgtccacaa attgtgatag cattttatga agagagactg acatggcatg catatcctga
     1801 ggatgcggaa aacaaagaga aagaaacagc aaagagctaa cctgcaggca tgcaagcttg
     1861 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     1921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     1981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2281 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2461 ccccgcatcg ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca
     2521 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     2581 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     2641 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2701 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2761 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat
     2821 taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
     2881 attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
     2941 tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
     3001 gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
     3061 gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg
     3121 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     3181 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     3241 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     3301 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa
     3361 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3421 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3481 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3541 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3601 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3661 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3721 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3781 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3841 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3901 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3961 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4021 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4081 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4141 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4201 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4261 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4321 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4381 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4441 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4501 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4561 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4621 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4681 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4741 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4801 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4861 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4921 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4981 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5041 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5101 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5161 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5221 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5281 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5341 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5401 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5461 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5521 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5581 cggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5641 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5761 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5821 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5881 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5941 agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca
     6001 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
     6061 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
     6121 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
     6181 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
     6241 gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg
     6301 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
     6361 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
     6421 gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
     6481 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
     6541 ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
     6601 ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt
     6661 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
     6721 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
     6781 caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt
     6841 aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca
     6901 cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc
     6961 taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt
     7021 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     7081 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     7141 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     7201 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     7261 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     7321 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     7381 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     7441 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     7501 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     7561 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     7621 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     7681 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     7741 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7801 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     7861 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     7921 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     7981 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     8041 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     8101 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     8161 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     8221 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     8281 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     8341 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     8401 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     8461 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8521 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8581 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8641 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     8701 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8761 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8821 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8881 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8941 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     9001 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     9061 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     9121 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     9181 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9241 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9301 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9361 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9421 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     9481 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     9541 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     9601 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     9661 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     9721 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     9781 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     9841 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     9901 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     9961 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
    10021 acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg
    10081 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10141 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    10201 gcagagatcc agtttggatc gataagcttg atatcgaatt ctaccgggta ggggaggcgc
    10261 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
    10321 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
    10381 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
    10441 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
    10501 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
    10561 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
    10621 ggctcagggg
//
