LOCUS       sequence-34350-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-Light SMR5452
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(189..293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     361..379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
       61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      301 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      361 aataggcgta tcacgaggcc ctggcgcgcc taggccatat gggcccgggc gagcatattg
      421 atccgctgca aactgaaatt ggcagcatgg tctatgcggt gaaaccaggc gatggttctg
      481 cgcgtgaaca ggcggcgagc tgccagcgtg tgattggcgg tctggcgaat attgccgagg
      541 agtacgcgac taaacgctat cgttctaacg tcatcaactg ggggatgtta ccgctgcaaa
      601 tggcggaagt accagccttt gaagtggggg attacattta catccctggc attaaagcgg
      661 cgctggataa tccgggtacg acgtttaaag gttatgtgat ccatgaagat gcgccggtaa
      721 cggaaattac gctctatatg gaaagtctga ctgctgaaga gcgcgagatt atcaaggcgg
      781 gtagtttgat taacttcaat aaaaaccgtc agatgtaaaa agcgccatgt gaatgtaggt
      841 cgcattcggc acttattgtc ggatgcgatg cttgcgcatc ttatccgacc tacgaatcgc
      901 atcgaatctg tag
//
