LOCUS       sequence-343500-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PYL1-sfGFP-HP1AI165E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Gao Y, Han M, Shang S, Wang H, Qi LS
  TITLE     Interrogation of the dynamic properties of higher-order 
            heterochromatin using CRISPR-dCas9.
  JOURNAL   Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034.
  PUBMED    34428454
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034."
COMMENT     Sequence Label: pHR: pPGK PYL1-sfGFP-HP1AI165E
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..85)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(70..86)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             176..409
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(434..452)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(435..452)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(742..761)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(975..994)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1094..1116
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1154..1172)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1240..1344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1345..1413,1414..2205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1563..1582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2376..2964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2865..2884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3118..3135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3205..3225)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3210..3539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3390..3525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4673..4738
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4868..4888
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5313..5447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5350..5369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5404..5423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5615..6248
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6295..6420
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6917..7150
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7335..7379
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7664..7781
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7846..8345
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7881..7904)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8278..8297
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8979..9689
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             8979..9689
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(9006..9034)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9629..9649
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(10321..10843,1..66)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10374..10394)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10792..10803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
       61 ccccgcatcg ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca
      121 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
      181 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
      241 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      301 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      361 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat
      421 taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
      481 attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
      541 tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
      601 gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
      661 gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg
      721 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      781 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
      841 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
      901 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa
      961 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     1021 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     1081 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     1141 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1201 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1261 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1321 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1381 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1441 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1501 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1561 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1621 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1681 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1741 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1801 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1861 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1921 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1981 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2041 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2101 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2161 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2221 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2281 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2521 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2761 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3001 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3061 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3121 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3181 cggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3361 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3541 agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca
     3601 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
     3661 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
     3721 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
     3781 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
     3841 gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg
     3901 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
     3961 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
     4021 gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
     4081 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
     4141 ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
     4201 ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt
     4261 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
     4321 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
     4381 caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt
     4441 aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca
     4501 cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc
     4561 taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt
     4621 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     4681 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     4741 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     4801 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     4861 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     4921 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     4981 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     5041 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     5101 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     5161 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     5221 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     5281 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     5341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     5461 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     5521 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     5581 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     5641 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     5701 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     5761 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     5821 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     5881 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     5941 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     6001 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     6061 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6121 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6181 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6241 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6301 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6361 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     6421 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     6481 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     6541 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     6601 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     6661 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     6721 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     6781 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6841 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6901 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6961 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     7021 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     7081 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     7141 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     7201 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     7261 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     7321 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7381 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7441 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     7501 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     7561 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     7621 acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7681 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7741 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7801 gcagagatcc agtttggatc gataagcttg atatcgaatt ctaccgggta ggggaggcgc
     7861 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     7921 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     7981 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     8041 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     8101 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     8161 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     8221 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     8281 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     8341 tttcgacctg cagcccaagc ttacgcgtct gcaggatatc aagcttgcgg tacgactgag
     8401 tcatgggtgg gggcgcgcca actcaagacg aattcaccca actctcccaa tcaatcgccg
     8461 agttccacac gtaccaactc ggtaacggcc gttgctcatc tctcctagct cagcgaatcc
     8521 acgcgccgcc ggaaacagta tggtccgtgg tgagacgttt cgataggcca cagatttaca
     8581 aacacttcat caaaagctgt aacgtgagtg aagatttcga gatgcgagtg ggatgcacgc
     8641 gcgacgtgaa cgtgataagt ggattaccgg cgaatacgtc tcgagagaga ttagatctgt
     8701 tggacgatga tcggagagtg actgggttta gtataaccgg tggtgaacat aggctgagga
     8761 attataaatc ggttacgacg gttcatagat ttgagaaaga agaagaagaa gaaaggatct
     8821 ggaccgttgt tttggaatct tatgttgttg atgtaccgga aggtaattcg gaggaagata
     8881 cgagattgtt tgctgatacg gttattagat tgaatcttca gaaacttgct tcgatcactg
     8941 aagctatgaa cgtggatcca ggtggaggtg gaagcggtag caaaggagaa gaacttttca
     9001 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     9061 tccgtggaga gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca
     9121 ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc
     9181 aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc
     9241 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc
     9301 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg
     9361 attttaaaga agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca
     9421 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc
     9481 acaacgttga agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg
     9541 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga
     9601 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
     9661 ttacacatgg catggatgag ctctacaaag gtggaggtcg gaccggatca ggaaagaaaa
     9721 ccaagcggac agctgacagt tcttcttcag aggatgagga ggagtatgtc gtggagaagg
     9781 tgctagacag gcgcgtggtt aagggacaag tggaatatct actgaagtgg aaaggctttt
     9841 ctgaggagca caatacttgg gaacctgaga aaaacttgga ttgccctgag ctaatttctg
     9901 aatttatgaa aaagtataag aagatgaagg agggtgaaaa taataaaccc agggagaagt
     9961 cagaaagtaa caagaggaaa tccaatttct caaacagtgc cgatgacatc aaatctaaaa
    10021 aaaagagaga gcagagcaat gatatcgctc ggggctttga gagaggactg gaaccagaaa
    10081 agatcattgg ggcaacagat tcctgtggtg atttaatgtt cctaatgaaa tggaaagaca
    10141 cagatgaagc tgacctggtt cttgcaaaag aagctaatgt gaaatgtcca caaattgtgg
    10201 aggcatttta tgaagagaga ctgacatggc atgcatatcc tgaggatgcg gaaaacaaag
    10261 agaaagaaac agcaaagagc taacctgcag gcatgcaagc ttgatatcaa gcttatcgat
    10321 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    10381 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    10441 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10501 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10561 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10621 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10681 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    10741 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10801 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgc
//
