LOCUS       sequence-343502-       10289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PYL1-sfGFP-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Gao Y, Han M, Shang S, Wang H, Qi LS
  TITLE     Interrogation of the dynamic properties of higher-order 
            heterochromatin using CRISPR-dCas9.
  JOURNAL   Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034.
  PUBMED    34428454
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034."
COMMENT     Sequence Label: pHR: pPGK PYL1-sfGFP-NLS
FEATURES             Location/Qualifiers
     source          1..10289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..77
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             759..1469
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             759..1469
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(786..814)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1409..1429
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1488..1508
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1549..2137
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1602..1622)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2020..2031)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2140..2156)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2141..2157)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2247..2480
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2505..2523)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2506..2523)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2813..2832)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3046..3065)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3165..3187
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3225..3243)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3311..3415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3416..3484,3485..4276)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3634..3653)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4447..5035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4936..4955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5189..5206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5276..5296)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5281..5610
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5461..5596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6744..6809
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6939..6959
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7384..7518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7421..7440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7475..7494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7686..8319
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8366..8491
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8988..9221
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9406..9450
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9735..9852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9917..10289,1..125)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9952..9975)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
       61 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
      121 tttcgacctg cagcccaagc ttacgcgtct gcaggatatc aagcttgcgg tacgactgag
      181 tcatgggtgg gggcgcgcca actcaagacg aattcaccca actctcccaa tcaatcgccg
      241 agttccacac gtaccaactc ggtaacggcc gttgctcatc tctcctagct cagcgaatcc
      301 acgcgccgcc ggaaacagta tggtccgtgg tgagacgttt cgataggcca cagatttaca
      361 aacacttcat caaaagctgt aacgtgagtg aagatttcga gatgcgagtg ggatgcacgc
      421 gcgacgtgaa cgtgataagt ggattaccgg cgaatacgtc tcgagagaga ttagatctgt
      481 tggacgatga tcggagagtg actgggttta gtataaccgg tggtgaacat aggctgagga
      541 attataaatc ggttacgacg gttcatagat ttgagaaaga agaagaagaa gaaaggatct
      601 ggaccgttgt tttggaatct tatgttgttg atgtaccgga aggtaattcg gaggaagata
      661 cgagattgtt tgctgatacg gttattagat tgaatcttca gaaacttgct tcgatcactg
      721 aagctatgaa cgtggatcca ggtggaggtg gaagcggtag caaaggagaa gaacttttca
      781 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      841 tccgtggaga gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca
      901 ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc
      961 aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc
     1021 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc
     1081 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg
     1141 attttaaaga agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca
     1201 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc
     1261 acaacgttga agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg
     1321 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga
     1381 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
     1441 ttacacatgg catggatgag ctctacaaag gtggaggtcg gaccggaccc aagaaaaaac
     1501 gcaaggtgta acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
     1561 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1621 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1681 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     1741 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     1801 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     1861 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     1921 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     1981 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2041 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     2101 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg
     2161 aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     2221 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     2281 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     2341 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2401 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2461 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     2521 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     2581 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     2641 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     2701 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     2761 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     2821 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     2881 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     2941 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     3001 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
     3061 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3121 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3181 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     3241 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3301 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3361 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3421 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3481 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3541 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3601 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3661 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3721 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3781 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3841 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3901 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3961 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4021 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4081 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4141 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4201 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4261 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4321 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4381 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4441 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4501 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4561 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4621 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4681 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4741 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4801 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4861 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4921 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4981 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5041 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5101 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5161 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     5221 cccaatacgc aaaccgcctc tccccgcgcg tcggccgatt cattaatgca gctgtggaat
     5281 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5341 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5401 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5461 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5521 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5581 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
     5641 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     5701 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     5761 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     5821 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     5881 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
     5941 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     6001 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     6061 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     6121 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
     6181 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
     6241 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     6301 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     6361 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     6421 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     6481 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     6541 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     6601 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     6661 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     6721 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     6781 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     6841 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     6901 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     6961 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     7021 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     7081 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     7141 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     7201 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     7261 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     7321 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     7381 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7441 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7501 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     7561 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     7621 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     7681 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     7741 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7801 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7861 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     7921 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7981 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8041 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8101 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8161 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8221 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8281 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     8341 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     8401 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     8461 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     8521 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     8581 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     8641 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     8701 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     8761 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     8821 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     8881 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8941 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9001 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9061 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9121 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9181 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9241 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9301 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9361 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9421 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9481 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9541 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9601 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     9661 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca
     9721 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     9781 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     9841 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt
     9901 gatatcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     9961 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
    10021 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
    10081 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
    10141 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
    10201 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
    10261 cagaggctgg gaaggggtgg gtccggggg
//
