LOCUS       sequence-343504-        9386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, 
            hg38).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Gao Y, Han M, Shang S, Wang H, Qi LS
  TITLE     Interrogation of the dynamic properties of higher-order 
            heterochromatin using CRISPR-dCas9.
  JOURNAL   Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034.
  PUBMED    34428454
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034."
COMMENT     Sequence Label: pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a...
FEATURES             Location/Qualifiers
     source          1..9386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(157..176)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        348..727
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        729..927
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     882..902
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             945..1125
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1172..1297
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1794..2027
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2212..2256
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2554..2671
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2924..2957)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2961..2981
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        3072..3090
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3142..3160
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        3268..4479
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4316..4335
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4495..4515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4894..5601
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5039..5057)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5307..5326
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5617..5673
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5677..6276
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5677..5696)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6173..6193
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6296..6329)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6385..6973
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6438..6458)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6856..6867)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6976..6992)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6977..6993)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7502..7682
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7744..8332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7824..7843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8503..9294,9295..9363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9126..9145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9364..9386,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
       61 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
      121 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
      181 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      241 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      301 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      361 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      421 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      481 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      721 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      781 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      841 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      901 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      961 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1021 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     1081 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1141 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1201 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1261 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1321 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1381 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1441 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1501 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1561 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1621 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1681 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1741 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1801 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1861 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1921 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1981 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2041 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2101 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2161 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2221 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2281 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2341 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2401 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2461 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     2521 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2581 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2641 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2701 agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag gcccgcgccg
     2761 gccccctcgc acggacttgt gggagaagct cggctactcc cctgccccgg ttaatttgca
     2821 tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc
     2881 tttttaatac tagctacatt ttacatgata ggcttggatt tctataactt cgtatagcat
     2941 acattatacg aagttataaa cagcacaaaa ggaaactcac cctaactgta aagtaattgt
     3001 gtgttttgag actataagta tcccttggag aaccaccttg ttgtgatatc acaggtttta
     3061 gagctaggcc aacatgagga tcacccatgt ctgcagggcc tagcaagtta aaataaggct
     3121 agtccgttat caacttggcc aacatgagga tcacccatgt ctgcagggcc aagtggcacc
     3181 gagtcggtgc ttttttctcg agtactagga tccattaggc ggccgcgtgg ataaccgtat
     3241 taccgccatg cattcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg
     3301 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
     3361 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
     3421 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
     3481 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
     3541 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
     3601 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
     3661 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
     3721 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
     3781 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
     3841 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
     3901 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
     3961 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
     4021 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
     4081 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     4141 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     4201 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     4261 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     4321 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     4381 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     4441 tccgctaaat tctggccgtt tttggctttt ttgttagacg ctagcgccac catgcccaaa
     4501 aagaaaagaa aagtgggtag tatggcttct aactttactc agttcgttct cgtcgacaat
     4561 ggcggaactg gcgacgtgac tgtcgcccca agcaacttcg ctaacgggat cgctgaatgg
     4621 atcagctcta actcgcgttc acaggcttac aaagtaacct gtagcgttcg tcagagctct
     4681 gcgcagaatc gcaaatacac catcaaagtc gaggtgccta aaggcgcctg gcgttcgtac
     4741 ttaaatatgg aactaaccat tccaattttc gccacgaatt ccgactgcga gcttattgtt
     4801 aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcagcaat cgcagcaaac
     4861 tccggcatct acggcggagg ttccgggccc gggatggtga gcaagggcga ggaggataac
     4921 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     4981 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     5041 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     5101 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     5161 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     5221 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     5281 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     5341 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     5401 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     5461 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     5521 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     5581 ggcatggacg agctgtacaa gaccggtgga agcggagcta ctaacttcag cctgctgaag
     5641 caggctggag acgtggagga gaaccctgga cctaccatga ccgagtacaa gcccacggtg
     5701 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5761 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5821 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5881 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5941 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6001 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6061 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6121 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6181 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6241 cgcacctggt gcatgacccg caagcccggt gcctgagaat tcgtcgaggg acctaataac
     6301 ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt ccactaggta
     6361 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     6421 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6481 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6541 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6601 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6661 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6721 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6781 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6841 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6901 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6961 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
     7021 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     7081 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     7141 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7201 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7261 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     7321 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     7381 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     7441 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     7501 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7561 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7621 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7681 cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8281 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8341 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8401 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8461 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8521 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8581 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8641 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8701 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8761 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8821 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8881 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8941 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9001 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9061 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9121 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9181 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9241 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9301 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9361 catactcttc ctttttcaat attatt
//
