LOCUS       sequence-343505-        9395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mMPP8 (deletion from D570 to K718) with 
            C-terminal Flag2 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, 
            Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K
  TITLE     MPP8 is essential for sustaining self-renewal of ground-state 
            pluripotent stem cells.
  JOURNAL   Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4.
  PUBMED    34031396
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-24"; volume: "12(1):3034. doi"; pages: " 
            10.1038/s41467-021-23308-4"
COMMENT     Sequence Label: pPB-MPP8-∆ARD_Flag2-Blast
FEATURES             Location/Qualifiers
     source          1..9395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(163..184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     375..394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     608..630
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     622..639
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     623..639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   683..717
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    992..1025
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1035..1414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1692..2708
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2632..2654
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2716..2735
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2772..2796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2811..2820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2815..2824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4950..4997
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     protein_bind    complement(5023..5047)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5199..5773,5774..5776,5777..5785)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5366..5383)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5593..5612
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6272..6291)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6337..6392
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6391..6410)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6753..6769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6777..6793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6801..6807,6808..6825,6826..6831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6846..6867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6940..6973
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   7144..7206
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7230..7248)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7269..7285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7293..7309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7317..7323,7324..7341,7342..7347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7362..7383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7500..7517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7671..8259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7751..7770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8430..9221,9222..9290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9053..9072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9291..9395)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
       61 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      121 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      181 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      241 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      301 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      361 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      421 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
      661 tagggcgaat tgggtacctt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
      721 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
      781 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
      841 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
      901 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
      961 attatatata tattttcttg ttatagatat cataacttcg tataatgtat gctatacgaa
     1021 gttatgcggc cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1381 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     1441 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     1501 attttgtgca gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg
     1561 gsgrggggsg gggsggggsg rggcggagag gkgcggcggc agccaatcag agcggcgcgc
     1621 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     1681 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     1741 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     1801 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     1861 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     1921 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     1981 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     2041 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     2101 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
     2161 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
     2221 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
     2281 cgggcggggc ggggccgcct cgggccgggg rgggctcggg ggaggggcgc ggcggccccc
     2341 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     2401 gcgagagggc gcagggactt tctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     2461 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     2521 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     2581 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     2641 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     2701 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     2761 tcctcgaatc aacaagtttg tacaaaaaag caggctccga attcgccctt gccaccatgg
     2821 cggcggcggc tgaggagggc atgagtgcgg ccgctctagt tatgtcagtt cccgacagca
     2881 ttggccgttc tcccgagtcc gaaggagttg gagcagggga cgaagagaag gacgcagcca
     2941 ccaaaggaac ggtggccgtt ggggacagcg aggaggatgg ggaggacgtt ttcgaggtgg
     3001 agaggatcct ggacatgaag tgcgagggag gtaagaatct ttataaagtt cgatggaaag
     3061 gatatacatc tgaagatgat acctgggagc ctgaggttca tctggaggac tgtaaggaag
     3121 ttcttcttga attcaggaag aaacttgctg agaacaaagc taaagcagtc aggaaagata
     3181 ttcagagact atctttaaac aatgacatat ttgaggcaga cagtgatagc gaccagcaaa
     3241 gtgacacaaa agaagatatt tcaccaagga agaaaaagaa aaaaataaag tgcaaagaag
     3301 agacaagtcc agaagatctg aggaaaaaaa ggacaaaaat gggaaaacta aaagacaagt
     3361 tcaagacaga gctggagagc acctctgaaa taatcggttt cgatgtaaag acgaagaaaa
     3421 gaatttggga agtcaaggaa gaattaaagg actccaaaaa gcccaaaaag gatgaaataa
     3481 aggaaaccaa ggaattaaag aaagctaata aaagggctga agtaagagat ttaaagatta
     3541 aaataagaga agatgtcaaa gagaaccgaa aaacaaaaaa agagagatat atagaatctc
     3601 cgctggagtc tgaatcgcct aatgattccc tcattctaga ggatgacagt gaagacttta
     3661 tttctgacaa cagagaagag aatcagaatg tgagaagtgt aagagataag acagcacagg
     3721 agacagtgca agagggtatt tttgagaaac atctggatga ccttataagt attgaagagg
     3781 atgctggtac ccgagttaga aggaagaaga cgaagccaag aaagtttgag gaaccaaaag
     3841 agatcaagaa gcttgaaagc acgaatgcct ttttagaaag gagagcaata cccaaaaagc
     3901 aaagaaacca agacaagggc ataagtaacc tagagttaaa taagctacca tcacctgtgt
     3961 ttgcccaaac cctgaaaagt tccagactga gtggggaaga gaagagccta aagtcccctg
     4021 acttggcaga agaggagaaa gagaaaaaaa atgaacccaa aggaaaatac cagaaaaggt
     4081 atgatttgga caaagaagaa aaagccagaa aagagccaaa ggtattaaaa tcatttaagg
     4141 aaatcagaaa tgcatttgat ttatttaaaa aaacaacaga agaaaaaaat gatgttcttg
     4201 agaataattc aaaaagagaa gaaatatcac tggattctaa aattatgaat gataacaaaa
     4261 ccaaggacaa gtgttcactt aaagaaaaaa gaaatactcg agatgagact gacacttggg
     4321 catacattgc tgcagaaggg gatcaggaag tttcagacag tgtatgccaa acagatgaga
     4381 cgtctgatgg caggcaacca gttttgagtt tgggtatgga cctgcagctg gagtggatga
     4441 aattagaaga ttttcagaag catctggatg gggaagatga gccctttatc acaacaaaca
     4501 ggattccaaa taatttgttg aggagccact tggagacact ttccagagtt gcagaagaaa
     4561 caatcaggga ttactttgaa tctcgccttg ctctactgga acctgttttc ccaatagcat
     4621 gccatcggct ctgtgagggg ccggatttct caacagattt caattacatg ccccctcaga
     4681 acatgcctga aggctctggt gtcctcctct ttattttcca tgcaaacttt ttgggcaaag
     4741 acgttattgc tcggctttgt ggaccatgca gtgtacaagc tgtggtttta aatgataaat
     4801 ttcaacttcc tgtttttctg gacagtcact ttgtttattc attcagtcct gttgctggcc
     4861 ccaataagtt gtttataagg ttgacggaag caccctttgc caaggttaag ttgctaatag
     4921 gtgcatacag agtacagctg cagggcgccg actacaagga cgacgatgac aaggactaca
     4981 aggacgacga tgacaagcgt accggttaga agggcgaatt cgacccagct ttcttgtaca
     5041 aagtggttga ttcgaggccg ctcgataagc ttgatatcga attcactcct caggtgcagg
     5101 ctgcctatca gaaggtggtg gctggtgtgg ccaactggct cacaaatacc actgagatcg
     5161 acggtatcga taagcttgat atcgaattcc gccccccccc cctctccctc ccccccccct
     5221 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     5281 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     5341 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     5401 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     5461 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     5521 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     5581 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     5641 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     5701 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     5761 acacgatgat aatatggcca caaccatgcc tttgtctcaa gaagaatcca ccctcattga
     5821 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     5881 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     5941 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     6001 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     6061 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     6121 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggttaact
     6181 agataagtaa tgatcataat cagccatatc acatctgtag aggttttact tgctttgtcc
     6241 gacgcggccc gacgggtccg aggggggtcg atctttttcc ctctgccaaa aattatgggg
     6301 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     6361 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     6421 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     6481 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     6541 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     6601 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     6661 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     6721 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6901 ggaaacctgt cgtgccagcg gatccgcggc cgcgtcgaga taacttcgta taatgtatgc
     6961 tatacgaagt tatgttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     7021 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     7081 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     7141 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     7201 ctagggttaa agagctccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     7261 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7321 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7381 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7441 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8101 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9361 tgtatttaga aaaataaaca aataggggtt ccgcg
//
