LOCUS       sequence-343506-        9386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 
            112319170, hg38).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Gao Y, Han M, Shang S, Wang H, Qi LS
  TITLE     Interrogation of the dynamic properties of higher-order 
            heterochromatin using CRISPR-dCas9.
  JOURNAL   Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034.
  PUBMED    34428454
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034."
COMMENT     Sequence Label: pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2...
FEATURES             Location/Qualifiers
     source          1..9386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(82..101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        273..652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        654..852
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     807..827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             870..1050
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1097..1222
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1719..1952
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2137..2181
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2479..2596
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2849..2882)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2886..2906
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        2997..3015
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3067..3085
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        3193..4404
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4241..4260
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4420..4440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4819..5526
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4964..4982)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5232..5251
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5542..5598
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5602..6201
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5602..5621)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6098..6118
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6221..6254)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6310..6898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6363..6383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6781..6792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6901..6917)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6902..6918)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7427..7607
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7669..8257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7749..7768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8428..9219,9220..9288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9051..9070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9289..9386,1..7))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
       61 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
      121 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
      181 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      241 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      301 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      361 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      421 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      481 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      541 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      601 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      661 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      721 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      781 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      841 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      901 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      961 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1021 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1081 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1141 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     1201 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     1261 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1321 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1381 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1441 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1501 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1561 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1621 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1681 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1741 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1801 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1861 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1921 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1981 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     2041 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     2101 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2161 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2221 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2281 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2341 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2401 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     2461 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2521 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2581 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     2641 gggcccgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga
     2701 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
     2761 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
     2821 acattttaca tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt
     2881 ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
     2941 aagtatccct tggagaacca ccttgttgac cattccttcg ttttagagct aggccaacat
     3001 gaggatcacc catgtctgca gggcctagca agttaaaata aggctagtcc gttatcaact
     3061 tggccaacat gaggatcacc catgtctgca gggccaagtg gcaccgagtc ggtgcttttt
     3121 tctcgagtac taggatccat taggcggccg cgtggataac cgtattaccg ccatgcattc
     3181 ggctccagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     3241 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     3301 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     3361 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     3421 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     3481 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     3541 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     3601 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     3661 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     3721 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     3781 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     3841 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     3901 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     3961 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     4021 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     4081 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     4141 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     4201 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     4261 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     4321 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     4381 ccgtttttgg cttttttgtt agacgctagc gccaccatgc ccaaaaagaa aagaaaagtg
     4441 ggtagtatgg cttctaactt tactcagttc gttctcgtcg acaatggcgg aactggcgac
     4501 gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg aatggatcag ctctaactcg
     4561 cgttcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa
     4621 tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt cgtacttaaa tatggaacta
     4681 accattccaa ttttcgccac gaattccgac tgcgagctta ttgttaaggc aatgcaaggt
     4741 ctcctaaaag atggaaaccc gattccctca gcaatcgcag caaactccgg catctacggc
     4801 ggaggttccg ggcccgggat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     4861 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     4921 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4981 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     5041 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     5101 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     5161 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     5221 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     5281 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     5341 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     5401 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     5461 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     5521 tacaagaccg gtggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     5581 gaggagaacc ctggacctac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5641 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5701 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5761 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5821 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5881 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5941 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6001 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6061 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6121 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6181 acccgcaagc ccggtgcctg agaattcgtc gagggaccta ataacttcgt atagcataca
     6241 ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag
     6301 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6361 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6421 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6481 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6541 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6601 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6661 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6721 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6781 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6841 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6901 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6961 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7021 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7081 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7141 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     7201 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7261 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     7321 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     7381 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     7441 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7501 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7561 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
     7621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8101 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9361 tatttagaaa aataaacaaa tagggg
//
