LOCUS       sequence-343507-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PYL1-sfGFP-HP1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Gao Y, Han M, Shang S, Wang H, Qi LS
  TITLE     Interrogation of the dynamic properties of higher-order 
            heterochromatin using CRISPR-dCas9.
  JOURNAL   Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034.
  PUBMED    34428454
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034."
COMMENT     Sequence Label: pHR: pPGK PYL1-sfGFP-HP1A
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..20)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5..21)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             111..344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(369..387)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(370..387)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(677..696)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(910..929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1029..1051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1089..1107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1175..1279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1280..1348,1349..2140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1498..1517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2311..2899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2800..2819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3053..3070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3140..3160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3145..3474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3325..3460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4608..4673
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4803..4823
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5248..5382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5285..5304)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5339..5358
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5550..6183
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6230..6355
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6852..7085
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7270..7314
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7599..7716
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7781..8280
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7816..7839)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8213..8232
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8914..9624
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             8914..9624
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(8941..8969)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9564..9584
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(10256..10843,1)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10309..10329)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10727..10738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 catcgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac
       61 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
      121 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
      181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
      361 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
      421 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
      481 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
      541 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
      601 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
      661 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
      721 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
      781 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
      841 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
      901 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      961 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     1021 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     1081 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1141 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1261 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1321 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1381 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1441 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1501 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1561 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1621 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1681 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1741 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1801 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1861 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1921 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1981 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2041 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2101 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3061 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgtcggcc
     3121 gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     3181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     3241 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3301 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3361 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3421 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     3481 ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag
     3541 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3601 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3661 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3721 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3781 ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
     3841 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
     3901 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
     3961 gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
     4021 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa
     4081 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
     4141 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
     4201 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
     4261 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
     4321 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
     4381 tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat
     4441 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
     4501 tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg
     4561 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     4621 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     4681 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     4741 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     4801 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     4861 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     4921 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
     4981 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
     5041 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
     5101 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     5161 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5221 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5281 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5341 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     5401 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     5461 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     5521 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     5581 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     5641 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     5701 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     5761 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     5821 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     5881 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     5941 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     6001 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6061 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6121 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6181 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     6241 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     6301 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     6361 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     6421 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     6481 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     6541 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     6601 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     6661 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     6721 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     6781 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     6841 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     6901 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     6961 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7021 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7081 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7141 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7201 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7261 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7321 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7381 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7441 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     7501 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     7561 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     7621 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     7681 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     7741 gatccagttt ggatcgataa gcttgatatc gaattctacc gggtagggga ggcgcttttc
     7801 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     7861 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     7921 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     7981 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     8041 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     8101 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     8161 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     8221 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     8281 acctgcagcc caagcttacg cgtctgcagg atatcaagct tgcggtacga ctgagtcatg
     8341 ggtgggggcg cgccaactca agacgaattc acccaactct cccaatcaat cgccgagttc
     8401 cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg aatccacgcg
     8461 ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat ttacaaacac
     8521 ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg cacgcgcgac
     8581 gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga tctgttggac
     8641 gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct gaggaattat
     8701 aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag gatctggacc
     8761 gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga agatacgaga
     8821 ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat cactgaagct
     8881 atgaacgtgg atccaggtgg aggtggaagc ggtagcaaag gagaagaact tttcactgga
     8941 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtccgt
     9001 ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat ttgcactact
     9061 ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg tgttcaatgc
     9121 ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa
     9181 ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa gacgcgtgct
     9241 gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt
     9301 aaagaagatg gaaacattct tggacacaaa ctcgagtaca actttaactc acacaatgta
     9361 tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac
     9421 gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc aattggcgat
     9481 ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct ttcgaaagat
     9541 cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca
     9601 catggcatgg atgagctcta caaaggtgga ggtcggaccg gatccggaaa gaaaaccaag
     9661 cggacagctg acagttcttc ttcagaggat gaggaggagt atgtcgtgga gaaggtgcta
     9721 gacaggcgcg tggttaaggg acaagtggaa tatctactga agtggaaagg cttttctgag
     9781 gagcacaata cttgggaacc tgagaaaaac ttggattgcc ctgagctaat ttctgaattt
     9841 atgaaaaagt ataagaagat gaaggagggt gaaaataata aacccaggga gaagtcagaa
     9901 agtaacaaga ggaaatccaa tttctcaaac agtgccgatg acatcaaatc taaaaaaaag
     9961 agagagcaga gcaatgatat cgctcggggc tttgagagag gactggaacc agaaaagatc
    10021 attggggcaa cagattcctg tggtgattta atgttcctaa tgaaatggaa agacacagat
    10081 gaagctgacc tggttcttgc aaaagaagct aatgtgaaat gtccacaaat tgtgatagca
    10141 ttttatgaag agagactgac atggcatgca tatcctgagg atgcggaaaa caaagagaaa
    10201 gaaacagcaa agagctaacc tgcaggcatg caagcttgat atcaagctta tcgataatca
    10261 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    10321 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    10381 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    10441 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    10501 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    10561 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    10621 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
    10681 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    10741 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    10801 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccg
//
