LOCUS       sequence-343509-        9841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full length mMPP8 with C-terminal Flag2 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, 
            Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K
  TITLE     MPP8 is essential for sustaining self-renewal of ground-state 
            pluripotent stem cells.
  JOURNAL   Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4.
  PUBMED    34031396
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-24"; volume: "12(1):3034. doi"; pages: " 
            10.1038/s41467-021-23308-4"
COMMENT     Sequence Label: pPB-MPP8_Flag2-Blast
FEATURES             Location/Qualifiers
     source          1..9841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(163..184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     375..394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     608..630
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     622..639
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     623..639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   683..717
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    992..1025
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1035..1414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1416..1690
                     /label=chicken beta-actin promoter
     intron          1691..2707
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2631..2653
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2715..2734
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2771..2795
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2810..2819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2814..2823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5396..5443
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     protein_bind    complement(5469..5493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5645..6219,6220..6222,6223..6231)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5812..5829)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6039..6058
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6718..6737)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6783..6838
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6837..6856)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7199..7215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7223..7239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7247..7253,7254..7271,7272..7277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7292..7313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7386..7419
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   7590..7652
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7676..7694)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7715..7731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7739..7755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7763..7769,7770..7787,7788..7793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7808..7829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7946..7963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8117..8705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8197..8216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8876..9667,9668..9736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9499..9518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9737..9841)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
       61 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      121 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      181 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      241 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      301 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      361 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      421 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
      661 tagggcgaat tgggtacctt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
      721 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
      781 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
      841 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
      901 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
      961 attatatata tattttcttg ttatagatat cataacttcg tataatgtat gctatacgaa
     1021 gttatgcggc cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1381 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     1441 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     1501 attttgtgca gcgatggggg cggggggggg ggggggcgcg ccrggsgggg sggggsgggg
     1561 sgrggggsgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     1621 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     1681 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     1741 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     1801 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     1861 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     1921 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     1981 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     2041 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     2101 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     2161 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     2221 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     2281 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     2341 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     2401 cgagagggcg cagggacttt ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     2461 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     2521 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     2581 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     2641 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     2701 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     2761 cctcgaatca acaagtttgt acaaaaaagc aggctccgaa ttcgcccttg ccaccatggc
     2821 ggcggcggct gaggagggca tgagtgcggc cgctctagtt atgtcagttc ccgacagcat
     2881 tggccgttct cccgagtccg aaggagttgg agcaggggac gaagagaagg acgcagccac
     2941 caaaggaacg gtggccgttg gggacagcga ggaggatggg gaggacgttt tcgaggtgga
     3001 gaggatcctg gacatgaagt gcgagggagg taagaatctt tataaagttc gatggaaagg
     3061 atatacatct gaagatgata cctgggagcc tgaggttcat ctggaggact gtaaggaagt
     3121 tcttcttgaa ttcaggaaga aacttgctga gaacaaagct aaagcagtca ggaaagatat
     3181 tcagagacta tctttaaaca atgacatatt tgaggcagac agtgatagcg accagcaaag
     3241 tgacacaaaa gaagatattt caccaaggaa gaaaaagaaa aaaataaagt gcaaagaaga
     3301 gacaagtcca gaagatctga ggaaaaaaag gacaaaaatg ggaaaactaa aagacaagtt
     3361 caagacagag ctggagagca cctctgaaat aatcggtttc gatgtaaaga cgaagaaaag
     3421 aatttgggaa gtcaaggaag aattaaagga ctccaaaaag cccaaaaagg atgaaataaa
     3481 ggaaaccaag gaattaaaga aagctaataa aagggctgaa gtaagagatt taaagattaa
     3541 aataagagaa gatgtcaaag agaaccgaaa aacaaaaaaa gagagatata tagaatctcc
     3601 gctggagtct gaatcgccta atgattccct cattctagag gatgacagtg aagactttat
     3661 ttctgacaac agagaagaga atcagaatgt gagaagtgta agagataaga cagcacagga
     3721 gacagtgcaa gagggtattt ttgagaaaca tctggatgac cttataagta ttgaagagga
     3781 tgctggtacc cgagttagaa ggaagaagac gaagccaaga aagtttgagg aaccaaaaga
     3841 gatcaagaag cttgaaagca cgaatgcctt tttagaaagg agagcaatac ccaaaaagca
     3901 aagaaaccaa gacaagggca taagtaacct agagttaaat aagctaccat cacctgtgtt
     3961 tgcccaaacc ctgaaaagtt ccagactgag tggggaagag aagagcctaa agtcccctga
     4021 cttggcagaa gaggagaaag agaaaaaaaa tgaacccaaa ggaaaatacc agaaaaggta
     4081 tgatttggac aaagaagaaa aagccagaaa agagccaaag gtattaaaat catttaagga
     4141 aatcagaaat gcatttgatt tatttaaaaa aacaacagaa gaaaaaaatg atgttcttga
     4201 gaataattca aaaagagaag aaatatcact ggattctaaa attatgaatg ataacaaaac
     4261 caaggacaag tgttcactta aagaaaaaag aaatactcga gatgagactg acacttgggc
     4321 atacattgct gcagaagggg atcaggaagt ttcagacagt gtatgccaaa cagatgagac
     4381 gtctgatggc aggcaaccag ttttgagttt gggtatggac ctgcagctgg agtggatgaa
     4441 attagaagat tttcagaagc atctggatgg ggaagatgag ccctttatca caacaaacag
     4501 gattccaaat aatttgttga gggatgctgt gaaaaatgga gattatattg ctgtgaaggt
     4561 tgcactgaac tcaaatgaag aatacaactt ggatcaagag gattctactg ggatgacgct
     4621 ggtgatgttg gctgcagcag gcggtcagga cgacctcctc agactcctta tcaccaaagg
     4681 tgcaaaagtg aatgggcgcc agaaaaacgg gactacagcc ctcattcatg ccgctgagaa
     4741 gaacttttta acaactgtag ctattctttt ggaagctgga gcttttgtga atgttcagca
     4801 aagcaatggt gagactgcgc tcatgaaagc atgcaaaaga ggaaattcgg acattgtccg
     4861 ccttgtgatt gaatgtggag ctgactgcaa cattttgtca aagcaccaaa atagtgcatt
     4921 gtactttgca aagcagtgta ataatgtgct tgtatatgaa ctgctgaaga gccacttgga
     4981 gacactttcc agagttgcag aagaaacaat cagggattac tttgaatctc gccttgctct
     5041 actggaacct gttttcccaa tagcatgcca tcggctctgt gaggggccgg atttctcaac
     5101 agatttcaat tacatgcccc ctcagaacat gcctgaaggc tctggtgtcc tcctctttat
     5161 tttccatgca aactttttgg gcaaagacgt tattgctcgg ctttgtggac catgcagtgt
     5221 acaagctgtg gttttaaatg ataaatttca acttcctgtt tttctggaca gtcactttgt
     5281 ttattcattc agtcctgttg ctggccccaa taagttgttt ataaggttga cggaagcacc
     5341 ctttgccaag gttaagttgc taataggtgc atacagagta cagctgcagg gcgccgacta
     5401 caaggacgac gatgacaagg actacaagga cgacgatgac aagcgtaccg gttagaaggg
     5461 cgaattcgac ccagctttct tgtacaaagt ggttgattcg aggccgctcg ataagcttga
     5521 tatcgaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5581 ctggctcaca aataccactg agatcgacgg tatcgataag cttgatatcg aattccgccc
     5641 cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5701 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5761 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5821 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5881 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5941 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6001 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     6061 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6121 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     6181 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catgcctttg
     6241 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     6301 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     6361 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     6421 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     6481 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     6541 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     6601 tctggttatg tgtgggaggg ttaactagat aagtaatgat cataatcagc catatcacat
     6661 ctgtagaggt tttacttgct ttgtccgacg cggcccgacg ggtccgaggg gggtcgatct
     6721 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     6781 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     6841 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     6901 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     6961 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     7021 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     7081 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     7141 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     7201 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7261 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7321 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcggccgcg
     7381 tcgagataac ttcgtataat gtatgctata cgaagttatg ttttgttact ttatagaaga
     7441 aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa
     7501 tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa
     7561 taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta
     7621 acgtacgtca caatatgatt atctttctag ggttaaagag ctccagcttt tgttcccttt
     7681 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7921 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7981 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8521 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9841 g
//
