LOCUS       sequence-343511-        7679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human H19 overexpression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Schultheiss CS, Laggai S, Czepukojc B, Hussein UK, List M, Barghash 
            A, Tierling S, Hosseini K, Golob-Schwarzl N, Pokorny J, Hachenthal 
            N, Schulz M, Helms V, Walter J, Zimmer V, Lammert F, Bohle RM, 
            Dandolo L, Haybaeck J, Kiemer AK, Kessler SM
  TITLE     The long non-coding RNA H19 suppresses carcinogenesis and 
            chemoresistance in hepatocellular carcinoma.
  JOURNAL   Cell Stress. 2017 Aug 25;1(1):37-54. doi: 10.15698/cst2017.10.105.
  PUBMED    31225433
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/cst2017.10"; journalName: "Cell Stress"; date: 
            "2017-08-25- 25"; volume: "1"; issue: "1"; pages: "37-54"
COMMENT     Sequence Label: pcDNa3.1(+)_A009-H19
FEATURES             Location/Qualifiers
     source          1..7679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        185..564
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        565..768
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     719..739
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     813..832
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        813..831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3220..3237)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3226..3450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3496..3924
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3583..3602)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3793..3814
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3933..3953)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3938..4267
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4118..4253
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4180..4199
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4334..5128
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4388..4407)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4998..5017
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5302..5423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5339..5358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5393..5412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5472..5488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5472..5488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5485..5507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5496..5512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5520..5526,5527..5544,5545..5550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5565..5586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5703..5720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5874..6462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5954..5973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6633..7424,7425..7493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7256..7275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7494..7598)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(7673..7679,1..13))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
       61 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
      121 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
      181 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      241 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      301 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      361 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      421 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      481 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      541 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      601 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      661 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      721 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      781 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      841 gctggctagc gtttaaactt aagcttggga gggggtggga tgggtggggg gtaacggggg
      901 aaactgggga agtggggaac cgaggggcaa ccaggggaag atggggtgct ggaggagagc
      961 ttgtgggagc caaggagcac cttggacatc tggagtctgg caggagtgat gacgggtgga
     1021 ggggctagct cgaggcaggg ctggtggggc ctgaggccag tgaggagtgt ggagtaggcg
     1081 cccaggcatc gtgcagacag ggcgacatca gctggggacg atgggcctga gctagggctg
     1141 gaaagaaggg ggagccaggc attcatcccg gtcacttttg gttacaggac gtggcagctg
     1201 gttggacgag gggagctggt gggcagggtt tgatcccagg gcctgggcaa cggaggtgta
     1261 gctggcagca gcgggcaggt gaggacccca tctgccgggc aggtgagtcc cttccctccc
     1321 caggcctcgc ttccccagcc ttctgaaaga aggaggttta ggggatcgag ggctggcggg
     1381 gagaagcaga caccctccca gcagaggggc aggatggggg caggagagtt agcaaaggtg
     1441 acatcttctc ggggggagcc gagactgcgc aaggctgggg ggttatgggc ccgttccagg
     1501 cagaaagagc aagagggcag ggagggagca caggggtggc cagcgtaggg tccagcacgt
     1561 ggggtggtac cccaggcctg ggtcagacag ggacatggca ggggacacag gacagagggg
     1621 tccccagctg ccacctcacc caccgcaatt catttagtag caggcacagg ggcagctccg
     1681 gcacggcttt ctcaggccta tgccggagcc tcgagggctg gagagcggga agacaggcag
     1741 tgctcgggga gttgcagcag gacgtcacca ggagggcgaa gcggccacgg gaggggggcc
     1801 ccgggacatt gcgcagcaag gaggctgcag gggctcggcc tgcgggcgcc ggtcccacga
     1861 ggcactgcgg cccagggtct ggtgcggaga gggcccacag tggacttggt gacgctgtat
     1921 gccctcaccg ctcagcccct ggggctggct tggcagacag tacagcatcc aggggagtca
     1981 agggcatggg gcgagaccag actaggcgag gcgggcgggg cggagtgaat gagctctcag
     2041 gagggaggat ggtgcaggca ggggtgagga gcgcagcggg cggcgagcgg gaggcactgg
     2101 cctccagagc ccgtggccaa ggcgggcctc gcgggcggcg acggagccgg gatcggtgcc
     2161 tcagcgttcg ggctggagac gaggccaggt ctccagctgg ggtggacgtg cccaccagct
     2221 gccgaaggcc aagacgccag gtccggtgga cgtgacaagc aggacatgac atggtccggt
     2281 gtgacggcga ggacagagga ggcgcgtccg gccttcctga acaccttagg ctggtggggc
     2341 tgcggcaaga agcgggtctg tttctttact tcctccacgg agtcggcaca ctatggctgc
     2401 cctctgggct cccagaaccc acaacatgaa agaaatggtg ctacccagct caagcctggg
     2461 cctttgaatc cggacacaaa accctctagc ttggaaatga atatgctgca ctttacaacc
     2521 actgcactac ctgactcagg aatcggctct ggaaggtgaa gctagaggaa ccagacctca
     2581 tcagcccaac atcaaagaca ccatcggaac agcagcgccc gcagcaccca ccccgcaccg
     2641 gcgactccat cttcatggcc accccctgcg gcggacggtt gaccaccagc caccacatca
     2701 tcccagagct gagctcctcc agcgggatga cgccgtcccc accacctccc tcttcttctt
     2761 tttcatcctt ctgtctcttt gtttctgagc tttcctgtct ttcctttttt ctgagagatt
     2821 caaagcctcc acgactctgt ttcccccgtc ccttctgaat ttaatttgca ctaagtcatt
     2881 tgcactggtt ggagttgtgg agacggcctt gagtctcagt acgagtgtgc gtgagtgtga
     2941 gccaccttgg caagtgcctg tgcagggccc ggccgccctc catctgggcc gggtgactgg
     3001 gcgccggctg tgtgcccgag gcctcaccct gccctcgcct agtctggaag ctccgaccga
     3061 catcacggag cagccttcaa gcattccatt acgccccatc tcgctctgtg cccctcccca
     3121 ccagggcttc agcaggagcc ctggactcat catcaataaa cactgttaca gcaaaaaaaa
     3181 aaaaaaaatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     3481 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3541 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3601 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3661 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3721 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3781 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3841 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3901 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     3961 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4021 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4081 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4141 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4201 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     4261 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     4321 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4381 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4441 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4501 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4561 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4621 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4681 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4741 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4801 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4861 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4921 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4981 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5041 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5101 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     5161 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5221 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5281 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     5341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5401 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     5461 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5641 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6301 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6541 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6601 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6661 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6721 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6781 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6841 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6901 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6961 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7021 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7081 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7141 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7201 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7261 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7321 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7381 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7441 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7501 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7561 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7621 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatc
//
