LOCUS       sequence-343512-        9454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, 
            Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K
  TITLE     MPP8 is essential for sustaining self-renewal of ground-state 
            pluripotent stem cells.
  JOURNAL   Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4.
  PUBMED    34031396
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-24"; volume: "12(1):3034. doi"; pages: " 
            10.1038/s41467-021-23308-4"
COMMENT     Sequence Label: pPB-MPP8-1–729_Flag2-Blast
FEATURES             Location/Qualifiers
     source          1..9454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(163..184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     375..394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     608..630
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     622..639
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     623..639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   683..717
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    992..1025
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1035..1414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1691..2707
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2631..2653
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2715..2734
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2771..2795
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2810..2819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2814..2823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5009..5056
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     protein_bind    complement(5082..5106)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5258..5832,5833..5835,5836..5844)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5425..5442)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5652..5671
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6331..6350)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6396..6451
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6450..6469)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6812..6828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6836..6852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6860..6866,6867..6884,6885..6890))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6905..6926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6999..7032
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   7203..7265
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7289..7307)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7328..7344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7352..7368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7376..7382,7383..7400,7401..7406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7421..7442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7559..7576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7730..8318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7810..7829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8489..9280,9281..9349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9112..9131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9350..9454)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
       61 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      121 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      181 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      241 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      301 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      361 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      421 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
      661 tagggcgaat tgggtacctt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
      721 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
      781 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
      841 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
      901 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
      961 attatatata tattttcttg ttatagatat cataacttcg tataatgtat gctatacgaa
     1021 gttatgcggc cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1381 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     1441 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     1501 attttgtgca gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg
     1561 gsgrgggggg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     1621 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     1681 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     1741 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     1801 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     1861 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     1921 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     1981 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     2041 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     2101 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     2161 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     2221 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     2281 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     2341 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     2401 cgagagggcg cagggacttt ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     2461 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     2521 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     2581 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     2641 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     2701 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     2761 cctcgaatca acaagtttgt acaaaaaagc aggctccgaa ttcgcccttg ccaccatggc
     2821 ggcggcggct gaggagggca tgagtgcggc cgctctagtt atgtcagttc ccgacagcat
     2881 tggccgttct cccgagtccg aaggagttgg agcaggggac gaagagaagg acgcagccac
     2941 caaaggaacg gtggccgttg gggacagcga ggaggatggg gaggacgttt tcgaggtgga
     3001 gaggatcctg gacatgaagt gcgagggagg taagaatctt tataaagttc gatggaaagg
     3061 atatacatct gaagatgata cctgggagcc tgaggttcat ctggaggact gtaaggaagt
     3121 tcttcttgaa ttcaggaaga aacttgctga gaacaaagct aaagcagtca ggaaagatat
     3181 tcagagacta tctttaaaca atgacatatt tgaggcagac agtgatagcg accagcaaag
     3241 tgacacaaaa gaagatattt caccaaggaa gaaaaagaaa aaaataaagt gcaaagaaga
     3301 gacaagtcca gaagatctga ggaaaaaaag gacaaaaatg ggaaaactaa aagacaagtt
     3361 caagacagag ctggagagca cctctgaaat aatcggtttc gatgtaaaga cgaagaaaag
     3421 aatttgggaa gtcaaggaag aattaaagga ctccaaaaag cccaaaaagg atgaaataaa
     3481 ggaaaccaag gaattaaaga aagctaataa aagggctgaa gtaagagatt taaagattaa
     3541 aataagagaa gatgtcaaag agaaccgaaa aacaaaaaaa gagagatata tagaatctcc
     3601 gctggagtct gaatcgccta atgattccct cattctagag gatgacagtg aagactttat
     3661 ttctgacaac agagaagaga atcagaatgt gagaagtgta agagataaga cagcacagga
     3721 gacagtgcaa gagggtattt ttgagaaaca tctggatgac cttataagta ttgaagagga
     3781 tgctggtacc cgagttagaa ggaagaagac gaagccaaga aagtttgagg aaccaaaaga
     3841 gatcaagaag cttgaaagca cgaatgcctt tttagaaagg agagcaatac ccaaaaagca
     3901 aagaaaccaa gacaagggca taagtaacct agagttaaat aagctaccat cacctgtgtt
     3961 tgcccaaacc ctgaaaagtt ccagactgag tggggaagag aagagcctaa agtcccctga
     4021 cttggcagaa gaggagaaag agaaaaaaaa tgaacccaaa ggaaaatacc agaaaaggta
     4081 tgatttggac aaagaagaaa aagccagaaa agagccaaag gtattaaaat catttaagga
     4141 aatcagaaat gcatttgatt tatttaaaaa aacaacagaa gaaaaaaatg atgttcttga
     4201 gaataattca aaaagagaag aaatatcact ggattctaaa attatgaatg ataacaaaac
     4261 caaggacaag tgttcactta aagaaaaaag aaatactcga gatgagactg acacttgggc
     4321 atacattgct gcagaagggg atcaggaagt ttcagacagt gtatgccaaa cagatgagac
     4381 gtctgatggc aggcaaccag ttttgagttt gggtatggac ctgcagctgg agtggatgaa
     4441 attagaagat tttcagaagc atctggatgg ggaagatgag ccctttatca caacaaacag
     4501 gattccaaat aatttgttga gggatgctgt gaaaaatgga gattatattg ctgtgaaggt
     4561 tgcactgaac tcaaatgaag aatacaactt ggatcaagag gattctactg ggatgacgct
     4621 ggtgatgttg gctgcagcag gcggtcagga cgacctcctc agactcctta tcaccaaagg
     4681 tgcaaaagtg aatgggcgcc agaaaaacgg gactacagcc ctcattcatg ccgctgagaa
     4741 gaacttttta acaactgtag ctattctttt ggaagctgga gcttttgtga atgttcagca
     4801 aagcaatggt gagactgcgc tcatgaaagc atgcaaaaga ggaaattcgg acattgtccg
     4861 ccttgtgatt gaatgtggag ctgactgcaa cattttgtca aagcaccaaa atagtgcatt
     4921 gtactttgca aagcagtgta ataatgtgct tgtatatgaa ctgctgaaga gccacttgga
     4981 gacactttcc agagttgcag aaggcgccga ctacaaggac gacgatgaca aggactacaa
     5041 ggacgacgat gacaagcgta ccggttagaa gggcgaattc gacccagctt tcttgtacaa
     5101 agtggttgat tcgaggccgc tcgataagct tgatatcgaa ttcactcctc aggtgcaggc
     5161 tgcctatcag aaggtggtgg ctggtgtggc caactggctc acaaatacca ctgagatcga
     5221 cggtatcgat aagcttgata tcgaattccg cccccccccc ctctccctcc ccccccccta
     5281 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     5341 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     5401 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5461 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5521 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5581 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5641 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5701 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5761 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     5821 cacgatgata atatggccac aaccatgcct ttgtctcaag aagaatccac cctcattgaa
     5881 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     5941 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     6001 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     6061 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     6121 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     6181 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggttaacta
     6241 gataagtaat gatcataatc agccatatca catctgtaga ggttttactt gctttgtccg
     6301 acgcggcccg acgggtccga ggggggtcga tctttttccc tctgccaaaa attatgggga
     6361 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     6421 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     6481 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     6541 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     6601 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     6661 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     6721 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     6781 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6961 gaaacctgtc gtgccagcgg atccgcggcc gcgtcgagat aacttcgtat aatgtatgct
     7021 atacgaagtt atgttttgtt actttataga agaaattttg agtttttgtt tttttttaat
     7081 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     7141 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     7201 cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc
     7261 tagggttaaa gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
     7321 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7381 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7441 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7501 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7561 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7621 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7681 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7741 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7801 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7861 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7921 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7981 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8041 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8101 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8161 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8221 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8281 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8341 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8401 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8461 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8521 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8581 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8641 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8701 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8761 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8821 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8881 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8941 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9001 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9061 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9121 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9181 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9241 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9301 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9361 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9421 gtatttagaa aaataaacaa ataggggttc cgcg
//
