LOCUS       sequence-343514-        9512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, 
            Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K
  TITLE     MPP8 is essential for sustaining self-renewal of ground-state 
            pluripotent stem cells.
  JOURNAL   Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4.
  PUBMED    34031396
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-24"; volume: "12(1):3034. doi"; pages: " 
            10.1038/s41467-021-23308-4"
COMMENT     Sequence Label: pPB-MPP8-112–858_Flag2-Blast
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(163..184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     375..394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     608..630
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     622..639
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     623..639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   683..717
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    992..1025
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1035..1414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1416..1691
                     /label=chicken beta-actin promoter
     intron          1692..2708
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2632..2654
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2716..2735
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2772..2796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2811..2820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5067..5114
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     protein_bind    complement(5140..5164)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5316..5890,5891..5893,5894..5902)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5483..5500)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5710..5729
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6389..6408)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6454..6509
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6508..6527)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6870..6886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6894..6910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6918..6924,6925..6942,6943..6948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6963..6984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7057..7090
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   7261..7323
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7347..7365)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7386..7402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7410..7426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7434..7440,7441..7458,7459..7464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7479..7500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7617..7634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7788..8376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7868..7887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8547..9338,9339..9407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9170..9189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9408..9512)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
       61 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      121 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      181 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      241 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      301 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      361 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      421 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
      661 tagggcgaat tgggtacctt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
      721 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
      781 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
      841 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
      901 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
      961 attatatata tattttcttg ttatagatat cataacttcg tataatgtat gctatacgaa
     1021 gttatgcggc cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1381 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     1441 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     1501 attttgtgca gcgatggggg cggggggggg gggggcgcgc gccrggsggg gsggggsggg
     1561 gsgrggggsg gggsggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
     1621 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     1681 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     1741 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     1801 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     1861 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     1921 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     1981 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     2041 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     2101 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
     2161 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
     2221 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
     2281 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc
     2341 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     2401 gcgagagggc gcagggactt tctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     2461 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     2521 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     2581 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     2641 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     2701 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     2761 tcctcgaatc aacaagtttg tacaaaaaag caggctccga attcgccctt gccaccatgg
     2821 agaacaaagc taaagcagtc aggaaagata ttcagagact atctttaaac aatgacatat
     2881 ttgaggcaga cagtgatagc gaccagcaaa gtgacacaaa agaagatatt tcaccaagga
     2941 agaaaaagaa aaaaataaag tgcaaagaag agacaagtcc agaagatctg aggaaaaaaa
     3001 ggacaaaaat gggaaaacta aaagacaagt tcaagacaga gctggagagc acctctgaaa
     3061 taatcggttt cgatgtaaag acgaagaaaa gaatttggga agtcaaggaa gaattaaagg
     3121 actccaaaaa gcccaaaaag gatgaaataa aggaaaccaa ggaattaaag aaagctaata
     3181 aaagggctga agtaagagat ttaaagatta aaataagaga agatgtcaaa gagaaccgaa
     3241 aaacaaaaaa agagagatat atagaatctc cgctggagtc tgaatcgcct aatgattccc
     3301 tcattctaga ggatgacagt gaagacttta tttctgacaa cagagaagag aatcagaatg
     3361 tgagaagtgt aagagataag acagcacagg agacagtgca agagggtatt tttgagaaac
     3421 atctggatga ccttataagt attgaagagg atgctggtac ccgagttaga aggaagaaga
     3481 cgaagccaag aaagtttgag gaaccaaaag agatcaagaa gcttgaaagc acgaatgcct
     3541 ttttagaaag gagagcaata cccaaaaagc aaagaaacca agacaagggc ataagtaacc
     3601 tagagttaaa taagctacca tcacctgtgt ttgcccaaac cctgaaaagt tccagactga
     3661 gtggggaaga gaagagccta aagtcccctg acttggcaga agaggagaaa gagaaaaaaa
     3721 atgaacccaa aggaaaatac cagaaaaggt atgatttgga caaagaagaa aaagccagaa
     3781 aagagccaaa ggtattaaaa tcatttaagg aaatcagaaa tgcatttgat ttatttaaaa
     3841 aaacaacaga agaaaaaaat gatgttcttg agaataattc aaaaagagaa gaaatatcac
     3901 tggattctaa aattatgaat gataacaaaa ccaaggacaa gtgttcactt aaagaaaaaa
     3961 gaaatactcg agatgagact gacacttggg catacattgc tgcagaaggg gatcaggaag
     4021 tttcagacag tgtatgccaa acagatgaga cgtctgatgg caggcaacca gttttgagtt
     4081 tgggtatgga cctgcagctg gagtggatga aattagaaga ttttcagaag catctggatg
     4141 gggaagatga gccctttatc acaacaaaca ggattccaaa taatttgttg agggatgctg
     4201 tgaaaaatgg agattatatt gctgtgaagg ttgcactgaa ctcaaatgaa gaatacaact
     4261 tggatcaaga ggattctact gggatgacgc tggtgatgtt ggctgcagca ggcggtcagg
     4321 acgacctcct cagactcctt atcaccaaag gtgcaaaagt gaatgggcgc cagaaaaacg
     4381 ggactacagc cctcattcat gccgctgaga agaacttttt aacaactgta gctattcttt
     4441 tggaagctgg agcttttgtg aatgttcagc aaagcaatgg tgagactgcg ctcatgaaag
     4501 catgcaaaag aggaaattcg gacattgtcc gccttgtgat tgaatgtgga gctgactgca
     4561 acattttgtc aaagcaccaa aatagtgcat tgtactttgc aaagcagtgt aataatgtgc
     4621 ttgtatatga actgctgaag agccacttgg agacactttc cagagttgca gaagaaacaa
     4681 tcagggatta ctttgaatct cgccttgctc tactggaacc tgttttccca atagcatgcc
     4741 atcggctctg tgaggggccg gatttctcaa cagatttcaa ttacatgccc cctcagaaca
     4801 tgcctgaagg ctctggtgtc ctcctcttta ttttccatgc aaactttttg ggcaaagacg
     4861 ttattgctcg gctttgtgga ccatgcagtg tacaagctgt ggttttaaat gataaatttc
     4921 aacttcctgt ttttctggac agtcactttg tttattcatt cagtcctgtt gctggcccca
     4981 ataagttgtt tataaggttg acggaagcac cctttgccaa ggttaagttg ctaataggtg
     5041 catacagagt acagctgcag ggcgccgact acaaggacga cgatgacaag gactacaagg
     5101 acgacgatga caagcgtacc ggttagaagg gcgaattcga cccagctttc ttgtacaaag
     5161 tggttgattc gaggccgctc gataagcttg atatcgaatt cactcctcag gtgcaggctg
     5221 cctatcagaa ggtggtggct ggtgtggcca actggctcac aaataccact gagatcgacg
     5281 gtatcgataa gcttgatatc gaattccgcc ccccccccct ctccctcccc cccccctaac
     5341 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5401 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5461 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5521 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5581 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5641 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5701 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5761 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5821 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5881 cgatgataat atggccacaa ccatgccttt gtctcaagaa gaatccaccc tcattgaaag
     5941 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     6001 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     6061 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     6121 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     6181 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     6241 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gttaactaga
     6301 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttgtccgac
     6361 gcggcccgac gggtccgagg ggggtcgatc tttttccctc tgccaaaaat tatggggaca
     6421 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     6481 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     6541 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     6601 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     6661 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     6721 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     6781 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     6841 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6901 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6961 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7021 aacctgtcgt gccagcggat ccgcggccgc gtcgagataa cttcgtataa tgtatgctat
     7081 acgaagttat gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     7141 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     7201 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     7261 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     7321 gggttaaaga gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     7381 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7441 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7501 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7561 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8221 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8401 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8461 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8521 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8581 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8641 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8701 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8761 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8821 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8881 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8941 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9001 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9061 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9121 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9181 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9241 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9301 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9361 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9421 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9481 atttagaaaa ataaacaaat aggggttccg cg
//
