LOCUS       sequence-343531-        2847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Acceptor for CDS to be N-tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2847)
  TITLE     Banfield lab plasmids
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICH41308
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    122..143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(158..163,164..181,182..188)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    196..212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     201..223
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     220..236
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     220..236
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             232..555
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(306..323)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(306..322)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(315..337)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(531..550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(692..710)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        778..882
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1104..1895
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2066..2654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2555..2574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2808..2825
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaaatgtt
       61 gtcttcgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      121 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      181 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      241 attacgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg
      301 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      361 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      421 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
      481 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      541 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacgaagac aagctttgag
      661 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
      721 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      781 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      841 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
      901 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      961 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1021 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1081 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     1141 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1201 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
     1261 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1321 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1381 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1441 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1501 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1561 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     1621 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     1681 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1741 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     1801 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg
     1861 taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata
     1921 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1981 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2701 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2761 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2821 gcgaggaagc ggaagagcgc ccaatac
//
