LOCUS       sequence-343535-        2847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Acceptor for CDS to be C-tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2847)
  TITLE     Banfield lab plasmids
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL01005
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..59)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        127..231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             453..1244
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1415..2003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1904..1923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2157..2174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2318..2339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2354..2359,2360..2377,2378..2384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2392..2408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2397..2419
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2416..2432
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2416..2432
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2428..2751
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    2445..2501
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2502..2519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2502..2518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2511..2533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2727..2746)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 attcgtgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt
       61 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
      241 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
      301 atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc
      361 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
      421 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
      481 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
      541 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
      601 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
      661 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
      721 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
      781 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
      841 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
      901 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
      961 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     1021 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     1081 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     1141 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     1201 tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt
     1261 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1321 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1381 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1441 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1501 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1561 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1621 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1681 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1741 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1801 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1861 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1921 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1981 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2041 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2101 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2161 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2221 ggccgattca ttaatcactc tgtggtctca aatgttgtct tcgcagctgg cacgacaggt
     2281 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     2341 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     2401 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc
     2461 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa
     2521 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2581 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2641 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2701 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2761 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2821 gcttacagac aagctgtgac gaagaca
//
