LOCUS       sequence-343536-       16795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EGFP and rtTA-InteinN in Rabies virus infected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Chen HS, Zhang XL, Yang RR, Wang GL, Zhu XY, Xu YF, Wang DY, Zhang 
            N, Qiu S, Zhan LJ, Shen ZM, Xu XH, Long G, Xu C
  TITLE     An intein-split transactivator for intersectional neural imaging and
            optogenetic manipulation.
  JOURNAL   Nat Commun. 2022 Jun 23;13(1):3605. doi: 10.1038/s41467-022-31255-x.
  PUBMED    35739125
REFERENCE   2  (bases 1 to 16795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-23"; volume: "13(1):3605. doi"; pages: " 
            10.1038/s41467-022-31255-x"
COMMENT     Sequence Label: pRabies-EGFP-rtTAn
FEATURES             Location/Qualifiers
     source          1..16795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4270..4279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4276..4278,4279..4281,4282..4992)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4276..4278,4279..4281,4282..4992)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4321..4342)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4582..4601)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4929..4950
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5002..5055
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7694..7705
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12803..12820)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12809..13033
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13079..13507
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13166..13185)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13376..13397
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13516..13536)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13521..13850
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13701..13836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13763..13782
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13898..13945
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13964..14338
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    14468..14589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14505..14524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14559..14578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14638..14654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14638..14654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14651..14673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14662..14678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14686..14692,14693..14710,14711..14716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14731..14752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14869..14886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15040..15628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15120..15139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15799..16590,16591..16659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16422..16441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16660..16764)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 ttaagcgtct gatgagtccg tgaggacgaa acccggagtc ccgggtcacg cttaacaacc
      961 agatcaaaga aaaaacagac attgtcaatt gcaaagcaaa aatgtaacac ccctacaatg
     1021 gatgccgaca agattgtatt caaagtcaat aatcaggtgg tctctttgaa gcctgagatt
     1081 atcgtggatc aatatgagta caagtaccct gccatcaaag atttgaaaaa gccctgtata
     1141 accctaggaa aggctcccga tttaaataaa gcatacaagt cagttttgtc aggcatgagc
     1201 gccgccaaac ttaatcctga cgatgtatgt tcctatttgg cagcggcaat gcagtttttt
     1261 gaggggacat gtccggaaga ctggaccagc tatggaattg tgattgcacg aaaaggagat
     1321 aagatcaccc caggttctct ggtggagata aaacgtactg atgtagaagg gaattgggct
     1381 ctgacaggag gcatggaact gacaagagac cccactgtcc ctgagcatgc gtccttagtc
     1441 ggtcttctct tgagtctgta taggttgagc aaaatatccg ggcaaaacac tggtaactat
     1501 aagacaaaca ttgcagacag gatagagcag atttttgaga cagccccttt tgttaaaatc
     1561 gtggaacacc atactctaat gacaactcac aaaatgtgtg ctaattggag tactatacca
     1621 aacttcagat ttttggccgg aacctatgac atgtttttct cccggattga gcatctatat
     1681 tcagcaatca gagtgggcac agttgtcact gcttatgaag actgttcagg actggtatca
     1741 tttactgggt tcataaaaca aatcaatctc accgctagag aggcaatact atatttcttc
     1801 cacaagaact ttgaggaaga gataagaaga atgtttgagc cagggcagga gacagctgtt
     1861 cctcactctt atttcatcca cttccgttca ctaggcttga gtgggaaatc tccttattca
     1921 tcaaatgctg ttggtcacgt gttcaatctc attcactttg taggatgcta tatgggtcaa
     1981 gtcagatccc taaatgcaac ggttattgct gcatgtgctc ctcatgaaat gtctgttcta
     2041 gggggctatc tgggagagga attcttcggg aaagggacat ttgaaagaag attcttcaga
     2101 gatgagaaag aacttcaaga atacgaggcg gctgaactga caaagactga cgtagcactg
     2161 gcagatgatg gaactgtcaa ctctgacgac gaggactact tttcaggtga aaccagaagt
     2221 ccggaggctg tttatactcg aatcatgatg aatggaggtc gactaaagag atctcacata
     2281 cggagatatg tctcagtcag ttccaatcat caagcccgtc caaactcatt cgccgagttt
     2341 ctaaacaaga catattcgag tgactcataa gaagttgaat aacaaaatgc cggaaatcta
     2401 cggattgtgt atatccatca tgaaaaaaac taacacccct cctttcgaac catcccaaac
     2461 atgagcaaga tctttgtcaa tcctagtgct attagagccg gtctggccga tcttgagatg
     2521 gctgaagaaa ctgttgatct gatcaataga aatatcgaag acaatcaggc tcatctccaa
     2581 ggggaaccca tagaggtgga caatctccct gaggatatgg ggcgacttca cctggatgat
     2641 ggaaaatcgc ccaaccatgg tgagatagcc aaggtgggag aaggcaagta tcgagaggac
     2701 tttcagatgg atgaaggaga ggatcctagc ttcctgttcc agtcatacct ggaaaatgtt
     2761 ggagtccaaa tagtcagaca aatgaggtca ggagagagat ttctcaagat atggtcacag
     2821 accgtagaag agattatatc ctatgtcgcg gtcaactttc ccaaccctcc aggaaagtct
     2881 tcagaggata aatcaaccca gactactggc cgagagctca agaaggagac aacacccact
     2941 ccttctcaga gagaaagcca atcatcgaaa gccaggatgg cggctcaaat tgcttctggc
     3001 cctccagccc ttgaatggtc ggctaccaat gaagaggatg atctatcagt ggaggctgag
     3061 atcgctcacc agattgcaga aagtttctcc aaaaaatata agtttccctc tcgatcctca
     3121 gggatactct tgtataattt tgagcaattg aaaatgaacc ttgatgatat agttaaagag
     3181 gcaaaaaatg taccaggtgt gacccgttta gcccatgacg ggtccaaact ccccctaaga
     3241 tgtgtactgg gatgggtcgc tttggccaac tctaagaaat tccagttgtt agtcgaatcc
     3301 gacaagctga gtaaaatcat gcaagatgac ttgaatcgct atacatcttg ctaaccgaac
     3361 ctctcccctc agtccctcta gacaataaaa tccgagatgt cccaaagtca acatgaaaaa
     3421 aacaggcaac accactgata aatcgatgaa cctcctacgt aagatagtga aaaaccgcag
     3481 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3541 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     3601 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     3661 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     3721 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     3781 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     3841 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     3901 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     3961 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4021 agaggacaaa gattcctctc tgcttctaga ataacgtacg tcagattata tcccgcaaat
     4081 ttatcacttg tttacctctg gaggagagaa catatgggct caactccaac ccttgggagc
     4141 aatataacaa aaaacatgtt atggtgccat taaaccgctg catttcatca aagtcaagtt
     4201 gattaccttt acattttgat cctcttggat gtgaaaaaaa ctattaacat ccctcaaagg
     4261 acctgcaggg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4321 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4381 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4441 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4501 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4561 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4621 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4681 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4741 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     4801 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4861 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     4921 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4981 gagctgtaca agggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     5041 gagaatcctg gcccatctag actggacaag agcaaagtca taaactctgc tctggaatta
     5101 ctcaatggag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     5161 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca
     5221 atcgagatgc tggacaggca tcatacccac tcctgccccc tggaaggcga gtcatggcaa
     5281 gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg
     5341 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     5401 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     5461 gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag
     5521 gaaagagaga cacctaccac cgattctatg cccccacttc tgaaacaagc aattgagctg
     5581 ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     5641 ctggagaaac agctaaagtg cgaaagcggc gggacccgca gcggctactg cctggacctg
     5701 aagacccagg tgcagacccc ccagggcatg aaggagatca gcaacatcca ggtgggcgac
     5761 ctggtgctga gcaacaccgg ctacaacgag gtgctgaacg tgttccccaa gagcaagaag
     5821 aagagctaca agatcaccct ggaggacggc aaggagatca tctgcagcga ggagcacctg
     5881 ttccccaccc agaccggcga gatgaacatc agcggcggcc tgaaggaggg catgtgcctg
     5941 tacgtgaagg agtaaggcgc gcctacccgc ggtagctttt cagtcgagaa aaaaacatta
     6001 gatcagaaga acaactggca acacttctca acctgagact tacttcaaga tgctcgatcc
     6061 tggagaggtc tatgatgacc ctattgaccc aatcgagtta gaggctgaac ccagaggaac
     6121 ccccattgtc cccaacatct tgaggaactc tgactacaat ctcaactctc ctttgataga
     6181 agatcctgct agactaatgt tagaatggtt aaaaacaggg aatagacctt atcggatgac
     6241 tctaacagac aattgctcca ggtctttcag agttttgaaa gattatttca agaaggtaga
     6301 tttgggttct ctcaaggtgg gcggaatggc tgcacagtca atgatttctc tctggttata
     6361 tggtgcccac tctgaatcca acaggagccg gagatgtata acagacttgg cccatttcta
     6421 ttccaagtcg tcccccatag agaagctgtt gaatctcacg ctaggaaata gagggctgag
     6481 aatcccccca gagggagtgt taagttgcct tgagagggtt gattatgata atgcatttgg
     6541 aaggtatctt gccaacacgt attcctctta cttgttcttc catgtaatca ccttatacat
     6601 gaacgcccta gactgggatg aagaaaagac catcctagca ttatggaaag atttaacctc
     6661 agtggacatc gggaaggact tggtaaagtt caaagaccaa atatggggac tgctgatcgt
     6721 gacaaaggac tttgtttact cccaaagttc caattgtctt tttgacagaa actacacact
     6781 tatgctaaaa gatcttttct tgtctcgctt caactcctta atggtcttgc tctctccccc
     6841 agagccccga tactcagatg acttgatatc tcaactatgc cagctgtaca ttgctgggga
     6901 tcaagtcttg tctatgtgtg gaaactccgg ctatgaagtc atcaaaatat tggagccata
     6961 tgtcgtgaat agtttagtcc agagagcaga aaagtttagg cctctcattc attccttggg
     7021 agactttcct gtatttataa aagacaaggt aagtcaactt gaagagacgt tcggtccctg
     7081 tgcaagaagg ttctttaggg ctctggatca attcgacaac atacatgact tggtttttgt
     7141 gtttggctgt tacaggcatt gggggcaccc atatatagat tatcgaaagg gtctgtcaaa
     7201 actatatgat caggttcacc ttaaaaaaat gatagataag tcctaccagg agtgcttagc
     7261 aagcgaccta gccaggagga tccttagatg gggttttgat aagtactcca agtggtatct
     7321 ggattcaaga ttcctagccc gagaccaccc cttgactcct tatatcaaaa cccaaacatg
     7381 gccacccaaa catattgtag acttggtggg ggatacatgg cacaagctcc cgatcacgca
     7441 gatctttgag attcctgaat caatggatcc gtcagaaata ttggatgaca aatcacattc
     7501 tttcaccaga acgagactag cttcttggct gtcagaaaac cgaggggggc ctgttcctag
     7561 cgaaaaagtt attatcacgg ccctgtctaa gccgcctgtc aatccccgag agtttctgag
     7621 gtctatagac ctcggaggat tgccagatga agacttgata attggcctca agccaaagga
     7681 acgggaattg aagattgaag gtcgattctt tgctctaatg tcatggaatc taagattgta
     7741 ttttgtcatc actgaaaaac tcttggccaa ctacatcttg ccactttttg acgcgctgac
     7801 tatgacagac aacctgaaca aggtgtttaa aaagctgatc gacagggtca ccgggcaagg
     7861 gcttttggac tattcaaggg tcacatatgc atttcacctg gactatgaaa agtggaacaa
     7921 ccatcaaaga ttagagtcaa cagaggatgt attttctgtc ctagatcaag tgtttggatt
     7981 gaagagagtg ttttctagaa cacacgagtt ttttcaaaag gcctggatct attattcaga
     8041 cagatcagac ctcatcgggt tacgggagga tcaaatatac tgcttagatg cgtccaacgg
     8101 cccaacctgt tggaatggcc aggatggcgg gctagaaggc ttacggcaga agggctggag
     8161 tctagtcagc ttattgatga tagatagaga atctcaaatc aggaacacaa gaaccaaaat
     8221 actagctcaa ggagacaacc aggttttatg tccgacatac atgttgtcgc cagggctatc
     8281 tcaagagggg ctcctctatg aattggagag aatatcaagg aatgcacttt cgatatacag
     8341 agccgtcgag gaaggggcat ctaagctagg gctgatcatc aagaaagaag agaccatgtg
     8401 tagttatgac ttcctcatct atggaaaaac ccctttgttt agaggtaaca tattggtgcc
     8461 tgagtccaaa agatgggcca gagtctcttg cgtctctaat gaccaaatag tcaacctcgc
     8521 caatataatg tcgacagtgt ccaccaatgc gctaacagtg gcacaacact ctcaatcttt
     8581 gatcaaaccg atgagggatt ttctgctcat gtcagtacag gcagtctttc actacctgct
     8641 atttagccca atcttaaagg gaagagttta caagattctg agcgctgaag gggagagctt
     8701 tctcctagcc atgtcaagga taatctatct agatccttct ttgggaggga tatctggaat
     8761 gtccctcgga agattccata tacgacagtt ctcagaccct gtctctgaag ggttatcctt
     8821 ctggagagag atctggttaa gctcccaaga gtcctggatt cacgcgttgt gtcaagaggc
     8881 tggaaaccca gatcttggag agagaacact cgagagcttc actcgccttc tagaagatcc
     8941 gaccacctta aatatcagag gaggggccag tcctaccatt ctactcaagg atgcaatcag
     9001 aaaggcttta tatgacgagg tggacaaggt ggaaaattca gagtttcgag aggcaatcct
     9061 gttgtccaag acccatagag ataattttat actcttctta atatctgttg agcctctgtt
     9121 tcctcgattt ctcagtgagc tattcagttc gtcttttttg ggaatccccg agtcaatcat
     9181 tggattgata caaaactccc gaacgataag aaggcagttt agaaagagtc tctcaaaaac
     9241 tttagaagaa tccttctaca actcagagat ccacgggatt agtcggatga cccagacacc
     9301 tcagagggtt gggggggtgt ggccttgctc ttcagagagg gcagatctac ttagggagat
     9361 ctcttgggga agaaaagtgg taggcacgac agttcctcac ccttctgaga tgttgggatt
     9421 acttcccaag tcctctattt cttgcacttg tggagcaaca ggaggaggca atcctagagt
     9481 ttctgtatca gtactcccgt cctttgatca gtcatttttt tcacgaggcc ccctaaaggg
     9541 atacttgggc tcgtccacct ctatgtcgac ccagctattc catgcatggg aaaaagtcac
     9601 taatgttcat gtggtgaaga gagctctatc gttaaaagaa tctataaact ggttcattac
     9661 tagagattcc aacttggctc aagctctaat taggaacatt atgtctctga caggccctga
     9721 tttccctcta gaggaggccc ctgtcttcaa aaggacgggg tcagccttgc ataggttcaa
     9781 gtctgccaga tacagcgaag gagggtattc ttctgtctgc ccgaacctcc tctctcatat
     9841 ttctgttagt acagacacca tgtctgattt gacccaagac gggaagaact acgatttcat
     9901 gttccagcca ttgatgcttt atgcacagac atggacatca gagctggtac agagagacac
     9961 aaggctaaga gactctacgt ttcattggca cctccgatgc aacaggtgtg tgagacccat
    10021 tgacgacgtg accctggaga cctctcagat cttcgagttt ccggatgtgt cgaaaagaat
    10081 atccagaatg gtttctgggg ctgtgcctca cttccagagg cttcccgata tccgtctgag
    10141 accaggagat tttgaatctc taagcggtag agaaaagtct caccatatcg gatcagctca
    10201 ggggctctta tactcaatct tagtggcaat tcacgactca ggatacaatg atggaaccat
    10261 cttccctgtc aacatatacg gcaaggtttc ccctagagac tatttgagag ggctcgcaag
    10321 gggagtattg ataggatcct cgatttgctt cttgacaaga atgacaaata tcaatattaa
    10381 tagacctctt gaattggtct caggggtaat ctcatatatt ctcctgaggc tagataacca
    10441 tccctccttg tacataatgc tcagagaacc gtctcttaga ggagagatat tttctatccc
    10501 tcagaaaatc cccgccgctt atccaaccac tatgaaagaa ggcaacagat caatcttgtg
    10561 ttatctccaa catgtgctac gctatgagcg agagataatc acggcgtctc cagagaatga
    10621 ctggctatgg atcttttcag actttagaag tgccaaaatg acgtacctat ccctcattac
    10681 ttaccagtct catcttctac tccagagggt tgagagaaac ctatctaaga gtatgagaga
    10741 taacctgcga caattgagtt ctttgatgag gcaggtgctg ggcgggcacg gagaagatac
    10801 cttagagtca gacgacaaca ttcaacgact gctaaaagac tctttacgaa ggacaagatg
    10861 ggtggatcaa gaggtgcgcc atgcagctag aaccatgact ggagattaca gccccaacaa
    10921 gaaggtgtcc cgtaaggtag gatgttcaga atgggtctgc tctgctcaac aggttgcagt
    10981 ctctacctca gcaaacccgg cccctgtctc ggagcttgac ataagggccc tctctaagag
    11041 gttccagaac cctttgatct cgggcttgag agtggttcag tgggcaaccg gtgctcatta
    11101 taagcttaag cctattctag atgatctcaa tgttttccca tctctctgcc ttgtagttgg
    11161 ggacgggtca ggggggatat caagggcagt cctcaacatg tttccagatg ccaagcttgt
    11221 gttcaacagt cttttagagg tgaatgacct gatggcttcc ggaacacatc cactgcctcc
    11281 ttcagcaatc atgaggggag gaaatgatat cgtctccaga gtgatagatc ttgactcaat
    11341 ctgggaaaaa ccgtccgact tgagaaactt ggcaacctgg aaatacttcc agtcagtcca
    11401 aaagcaggtc aacatgtcct atgacctcat tatttgcgat gcagaagtta ctgacattgc
    11461 atctatcaac cggatcaccc tgttaatgtc cgattttgca ttgtctatag atggaccact
    11521 ctatttggtc ttcaaaactt atgggactat gctagtaaat ccaaactaca aggctattca
    11581 acacctgtca agagcgttcc cctcggtcac agggtttatc acccaagtaa cttcgtcttt
    11641 ttcatctgag ctctacctcc gattctccaa acgagggaag tttttcagag atgctgagta
    11701 cttgacctct tccacccttc gagaaatgag ccttgtgtta ttcaattgta gcagccccaa
    11761 gagtgagatg cagagagctc gttccttgaa ctatcaggat cttgtgagag gatttcctga
    11821 agaaatcata tcaaatcctt acaatgagat gatcataact ctgattgaca gtgatgtaga
    11881 atcttttcta gtccacaaga tggttgatga tcttgagtta cagaggggaa ctctgtctaa
    11941 agtggctatc attatagcca tcatgatagt tttctccaac agagtcttca acgtttccaa
    12001 acccctaact gacccctcgt tctatccacc gtctgatccc aaaatcctga ggcacttcaa
    12061 catatgttgc agtactatga tgtatctatc tactgcttta ggtgacgtcc ctagcttcgc
    12121 aagacttcac gacctgtata acagacctat aacttattac ttcagaaagc aagtcattcg
    12181 agggaacgtt tatctatctt ggagttggtc caacgacacc tcagtgttca aaagggtagc
    12241 ctgtaattct agcctgagtc tgtcatctca ctggatcagg ttgatttaca agatagtgaa
    12301 gactaccaga ctcgttggca gcatcaagga tctatccaga gaagtggaaa gacaccttca
    12361 taggtacaac aggtggatca ccctagagga tatcagatct agatcatccc tactagacta
    12421 cagttgcctg tgaaccggat actcctggaa gcctgcccat gctaagactc ttgtgtgatg
    12481 tatcttgaaa aaaacaagat cctaaatctg aacctttggt tgtttgattg tttttctcat
    12541 ttttgttgtt tatttgttaa gcgtgggtcg gcatggcatc tccacctcct cgcggtccga
    12601 cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggagaa gggcgaattc
    12661 cagcacactg gcggccgtta ctagtggatc cgagctcggt accgagctcg gatccactag
    12721 tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg
    12781 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    12841 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    12901 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    12961 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    13021 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
    13081 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    13141 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    13201 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    13261 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    13321 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    13381 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    13441 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    13501 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
    13561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    13621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    13681 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    13741 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
    13801 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
    13861 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
    13921 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
    13981 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
    14041 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
    14101 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
    14161 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
    14221 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
    14281 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    14341 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    14401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    14461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    14521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    14581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    14641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    14701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    14761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    14821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    14881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    14941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    15001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    15061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    15121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    15181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    15241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    15301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    15361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    15421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    15481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    15541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    15601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    15661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    15721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    15781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    15841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    15901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    15961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    16021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    16081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    16141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    16201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    16261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    16321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    16381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    16441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    16501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    16561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    16621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    16681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    16741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
