LOCUS       sequence-34354-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-Express-Tet SMR5918
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agctatttag gtgacactat agaatactca agcttgcatg cgtctagagc tcgaggtaga
       61 tctgaaagct tgaatcgatg tacgtactat accggtgcga acgggaaggt aaaaagacaa
      121 aaagttgttt ttaatacctt taagtgatac cagatggcat tgcgccatct ggcagagtga
      181 ttaactaaac atcgcagtaa tcgaggcgct tgccagagag tggaaatgaa cgttaaaccc
      241 gaccatcgcg ccgctggcac cttcatcgac atcaatacgt tctatatcca gcgcgtgaac
      301 ggtaaaaatg tagcgatgag tttcgccttt cggcggtgct gcgccatcgt acccggtttt
      361 accaaagtcg gtacgcgtct gcaaaacgcc gtctggcatt gctaccagac cagagccaaa
      421 cccttgcggt aatacgcggg tatcagcggg taagttaaca actacccagt gccaccagcc
      481 ggagccggtt ggcgcatccg ggtcgtagca ggtgacaaca aaacttttcg ttcccgcagg
      541 aacatcatcc cacgccagat gcggtgaaat attatcgcca tcgtaaccca tgccgttaaa
      601 gacatgacga tgcggcaatt tatcgccatc gcgcagatcg ttactgatga gtttcatgaa
      661 ccctcctttc ttgtttgcag aaagtgtagc cagaaaccct cacgcggact tctcgttatt
      721 ggcaaaaaaa tgtttcatcc tgtaccgcgc ggttaaccgc tgcggtcaga cgctgcaact
      781 gttgcgggag aataatatag ggcggcatca ggtaaatcag tttgccaaaa ggccggatcc
      841 agacaccctg ttcgacaaag aatttttgca gcgccgccat attcaccgga tgagtggttt
      901 cgaccacgcc aatggccccc agtacgcgca catcggcaac catttcg
//
