LOCUS       sequence-343543-       15674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15674)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 15674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD73
FEATURES             Location/Qualifiers
     source          1..15674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(46..634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(126..145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(805..1596,1597..1665))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1428..1447
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1666..1770)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1838..1856
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1894..1916)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    2239..2259
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(3766..3799)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(3799..3810)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(5232..5555)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    7473..7497
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7500..8525
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7500..8525
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8526..8550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8903..8936)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     9305..9322
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9306..9322
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             12630..12695
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             13551..13682
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             13683..13709
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      13718..14041
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(14074..14090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14074..14090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(15563..15583)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ccagcaaaag accaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
       61 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      121 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      181 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      241 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      301 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      361 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      421 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      481 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      541 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      601 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      661 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      721 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      781 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      841 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      901 acgggagggc ttaccatctg accccagtgc tgcaatgata ccgcgagacc cacgctcacc
      961 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1021 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1081 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1141 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1201 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1261 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1321 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1381 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1441 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1501 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1561 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1621 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1681 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1741 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     1801 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     1861 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     1921 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1981 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgcactgag
     2041 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2101 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2161 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2221 agggatccgc caagctatca actttgtata gaaaagttgc aagtggggat caggaagaag
     2281 ttgataaagt gattcaaaaa ctcgaggagc tcaacacagc gatgaaccca attttgagag
     2341 caacactgac gaagaactat gacgcgaggg taggcactac cttgaagcta tccacctgcc
     2401 tgcctgccgc ctatttccat gtgcctactg cgcatggaaa taggcggtag tcaggcaggt
     2461 agatagcctc ataaaattca atacttcctt ttcagaaaga atgctggaat gtattcaaag
     2521 aggagtcact cttcccattg ctcagagaat atgaagagtt gctcggcgag caaaagttca
     2581 tgattggaga tcgatattca tgggctgaca tcgctgtcgt cgagtttcta actcggtgtc
     2641 agcagtgcta cgatagcttc tatctggctc atttcgagag gctccgaact ttttgccaga
     2701 cttttgagtc tcttccgcac attcgtccct acattcaggg acgtgttgat tcatttatct
     2761 aattttttaa aatggatatt tttcgttttt tatttgtttg ttttgctgat aattcaataa
     2821 agttacgcaa cgaattttag acatttatga aaaccgaaaa tcgacaaaaa tattttaaga
     2881 aaaatagcag caaaattcaa tattttatca aattttgttt atggtaatga gaaagaaaac
     2941 atacaatatt tttctaagaa gaagaaaaat gaccagaatt ttaagatttt tctcgaatat
     3001 acgaaaaatt gataaactaa aattttcgat ttttccattt attttgaaaa aaaaactctt
     3061 gacggttttc accatgcgaa gaggtgagaa atattctgaa agttatgcat tttgaaaaaa
     3121 ttgcaaaaaa atacgttaaa attttttgat ctattgtttt tttttgaaaa ccggaattta
     3181 aaatggaatt ttttttcaaa aattaaaatt aaaatttaaa tatttctagt gtttggatag
     3241 tttttcaaaa gctagattta acccgcgaga tgtcgaccgc tagtgtagct tacagatggc
     3301 tgaagacttc gcaaaaatct tatcttagaa acccgcgagg tgtcggccgc taaaactttg
     3361 taaacctgcc aacaaaatgc ttcgcattgt ctgaacagat gaaaccttga aacaaaacat
     3421 atacaattga aatcccgtaa agtgtcgggc gcaagaactg cgattagttg tataattgga
     3481 ctagctcaaa tttgaccaaa atctcatttt tagacctcta agatgtcggc cactataact
     3541 ttattcaact tgaaactgca aaaaaactta tcaactgtat agattcaagt attatgtcaa
     3601 gtattggttt tagagctaca gtacactctg ataacaaaat ctgcagaaat acctccctgt
     3661 caattcccaa aatacctccc ctcatctcaa ttatcccgaa aatcggttag tcattgccat
     3721 cagaaaatcg aattaggtac catgatggta gcaaactcac ttcgtataac ttcgtatagt
     3781 ataccttata cgaagttatc tgccctcgat tgtctatctt ccattctcac caagtttcaa
     3841 caactttatt cagtcattct tctctggttc atttcccaac caatcttccc gaataaagcc
     3901 catctgtcct ttcggccaca ttcctcacct acatccgtac tccttatctc ccgttcttat
     3961 ccagtctttt atcgtctctg cgtctcgcac actgtctcgt cggtaatgta aaaaaaaaac
     4021 aaaaaaaact tgaaaacttg cggaaagatt tcctaataaa aggagacgaa tccggtagga
     4081 aatcgacaag tctctgcttc tcgaagacta ggtgagttac atttctttgg ccgaattatt
     4141 ttgaaatcct aaatttgata atttcagaga tgtctgtaaa acttgcgtgt tatgtgacgg
     4201 cttcggtcac tgtcgccact cttatggtat gcttcatgac catgtcaacc atctactcgg
     4261 aggttgatgg attcagagag aagcttgata ccgagatgaa tgtgttcaga gtgagttaaa
     4321 ttatattttg aattttaaat aatttttaat tttctagcaa tctaccaatg gattgtggaa
     4381 ggacatagtt gtcatcggaa gatctagcaa gcgtgtccgt tgtcaatatg aagagaccaa
     4441 cgctacccca actccacatg ctgatggatc cccatctgct ccaccaggtc aaccaccagc
     4501 agttccacca gtcttcaacc agccaaagac tccaaatgga gccaatggaa atggaccaac
     4561 ctgcaactgc aatgctgata acaagtgccc agctggacca tccggaccaa agggagttcc
     4621 aggagttcca ggactcgacg gagttccagg acttgacggt gttccaggag ttggagctga
     4681 tgatatcgct ccacaacgcg agtctgtcgg atgcttcact tgcccacaag gaccagttgg
     4741 accaccagga gctcttggaa gaccaggacc acgtggactt ccaggaccaa gaggacaaaa
     4801 tggaaaccca ggaagagatg gacaaccagg acatccagga gagcaaggat catccggtca
     4861 aatcggaaag atcggagagc caggaccacc aggagagaag ggacgcgacg ccgagcatcc
     4921 aatcggaaga ccaggaccaa agggaccaag aggagatcaa ggaccaacag gaccagctgg
     4981 acagaacggt cttcacggac caccaggaga gccaggaacc gttggaccag aaggaccatc
     5041 tggaaagcaa ggacgtcaag gaccagacgg aacccaggga gagactggac cagacggaag
     5101 accaggaaag gatgccgagt actgccagtg cccagacaag tctccaccat cagaggctgt
     5161 caacgccaac cgtggataca gaaatatcta aattgttggt gttttctaat aaaatatttg
     5221 agattcagtg agactttttt cttggcggca caataaagtt tcctcttttt gttgggtcac
     5281 tcaacgaaca caatacaaat atttgagcgc tcgcattttt ctcaaccttt cgggtttaaa
     5341 gcacttcaaa atggaattga agagagattg tgaaagtaca tcagacagaa tcacattttg
     5401 atagcattca cttcactcag atgcaaactt ttcagtgact ataaaaagtc ataaaataat
     5461 aacattaaaa aagcaaataa attagcgaga gaattttttg acaaaaagaa agaatagtga
     5521 tagagaagaa gggaatgctt gaaaggattt tgcatttatc ttaatcaccg tcttcgagaa
     5581 gacgaaccat ggcgccagtt tcagaatcca agtttctaat atagttcatc acaatgttga
     5641 cgttggtcca acctccggct tgcataatct ctgggatgga aactccagcg cgagccatgt
     5701 cacgggcagc tccgacacgg gcggaatgtc cagaccaagc caagtaacgt tgtccggaat
     5761 catccttggc tccgtagatg agacggtggg tagcttcgaa gattcctgaa aatttatttt
     5821 cagcttattc ttagctaata tgttgtttaa acttaccttc aagagctcga gtcgagagtt
     5881 gtgatgttgc actcggggca gcaactccgt tcttgcgaac acgacagaac aggtagttgt
     5941 ttggatcatc ggcgactccg gaaaccgaaa tccagcgctc aacgagcttg gtaactccaa
     6001 gggagagcgc tttttcgact ccggcagtcg agaccagcgt ttttgtacgt ccgatgtgga
     6061 tgagcattct tcctccgtct gtgcgactaa tatccttgac gcgaatgcgg gcaatttcgg
     6121 cgatacggag aagtgtgttg taagcaattc cgaggaaggc gaggtttcgg atgtcctggc
     6181 aacgatcgga attttccata agagaacgga cctgatcgaa gtcggttctg aaaatttcgt
     6241 caagtcttag gattgaatac attaatattt aaacttacct ttcgaaggcg agggcttgct
     6301 tggctctctc tccagcatca acattttcct tgcggatacg tctcattacg agagatacgg
     6361 cattagaatc tgatgggcgt ggaagtccag atcggcgatg gagcatattg agctgtccga
     6421 gatgttgttg gatcgtcttc accgccaatc cacgagcttg gaggtagagc aagtagtcac
     6481 gaacgtcttc tggctcggct gggaaccact tgcggttgtt aagcttgcac caggcagccc
     6541 aagaacggca cactgagaga agcatcttcc aggtgtgctc ggagaaggct tgtctgtcac
     6601 ggaacatatc catgaggttc ttgcgaactt catcacttgt cgcgtcaact ggaagtgctg
     6661 gaagattttg atgaacagtg agaagatttg acatattttc gaagtttttt agatgcacta
     6721 gaacaaagcg tgttggcttc ctctgagccc gctttcctta tatacccgca ttctgcagcc
     6781 ttacagaatg ttctagaagg tcctagatgc attcgtttga aaatactccc ggtgggtgca
     6841 aagagacgca gacggaaaat gtatctgggt ctctttattg tgtacactac ttttccatgt
     6901 accgaatgtg agtcgccctc cttttgcaac aagcagctcg aatgttctag aaaaaggtgg
     6961 aaaatagtat aaataccgtt gaaaataaat accgaacaac atttgctcta attgtgaaat
     7021 tagaaatctt caaactataa tcatgtcact ttaccactat ttccgtccag ctcaacgttc
     7081 cgtttttggt gatcattttt gctttcgtcg taaatctaca cacgcgtctc ttccgtgcga
     7141 gagtccaagc cagcagccaa attcgttgac tgagtattca acgtttatac gttgtcggca
     7201 acgagaaata ggaaaatgca tcgggaaatg ttcttttttc gattttttcc aaggttttga
     7261 caaattttac cacgaatttt gctatgtttt caattaaaaa atatgttatt caactgtttc
     7321 tatgaggaaa ataaggcttt gcatgtaatt ttcttattca gcataatttt taattaattt
     7381 gaattttctg tcctaacgtt tattttgttt tcttggttat gactgatctg aaattaattt
     7441 ttgaatttta aggtaatatg tcaggcggtg ccgcaagttt gtacaaaaaa gcaggctcca
     7501 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     7561 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     7621 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     7681 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     7741 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     7801 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     7861 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     7921 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     7981 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     8041 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     8101 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     8161 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     8221 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     8281 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     8341 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     8401 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     8461 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     8521 aatagaccca gctttcttgt acaaagtggg tccaattact cttcaacatc cctacatgct
     8581 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc
     8641 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     8701 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     8761 tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa
     8821 atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt
     8881 cagtttatga ccgcaatttt taataacttc gtatagtata ccttatacga agttatacta
     8941 gtatatattt ttatgttttc ttttaaacct tcctccatat ttctgaattg aatgttgttg
     9001 catattgtgt gtccttattt catttccatc atccttttgt gtgtttcccc cttccaccaa
     9061 ttccccccac gtcctaatga aatgaaagcg agaagataaa taaacttgca cattgtgttc
     9121 agatgagagg agcggaatac gaattgggag acggaaagag aaaaaaaaat gtttagattt
     9181 ggattgaatt aattttttag aaagcaagaa aaaaaacaca aagagaataa ataaataata
     9241 ggaaaaaaaa caaatattca gtatttaaaa tttgaaaatt caacgacgtt ggcgtcgatc
     9301 atcctgtaaa acgacggcca gtgcatgaac tcgactttaa tattcttttc tggtttcacc
     9361 agaaaaatgc tggactgaaa aattgaattt cccgcgattt gggaaaaacc gcagtttaag
     9421 cttttggcat gaatacagtg aacacaggaa caaaaaattg acttgttatt ttgagtttat
     9481 attaaatgtt aattttgcat ctcatatttt cacaaaactt gtttgcttga aaaatgtgca
     9541 aataacaagt gaacatacat caaggtttat gcagatcagc actgatgcaa ctgatagcaa
     9601 aactgataag ggtcaaaatt tcagtggcca gcacaaacac atacacattc gtagttcgtt
     9661 ttgtgtttcg tgtatttagc atttattttt caattccggc aatttgccga tttgccggaa
     9721 attttgattt ttggcaaatt gccagtttgc cgtttgccga acatcaattt gccgaaattt
     9781 tttagaggga tctttataag acggaaacac ttaaaattgt gcttttttgg aatttttttc
     9841 aatgtttcaa tagaattagc ttacatttaa aattagatgt aggaacgttt ataggacgct
     9901 tacaattttg ccgattaaaa tcgaagttct gaacttttca aaaaatgtgc aaaatttttt
     9961 ttgacaaatt tgacaaatcg gccggtttgt cgattgtccg gaatttttca gtttcggcga
    10021 tttaccggtt tgccggaaaa catcgtttgc cacccacaca cacataaaga gacgaaacaa
    10081 cacaccatct tcaaaaaatt tgtgaatctt catttccacg tggtttgcag ggtttcccat
    10141 ccaccccact ctatctctcc aagttgaatt ttggcaaaat tgaccacaca ccgatcaaaa
    10201 actattgtgt gtgtgtgtat ttgtgtcttc aataattcct gtctgtgtct cgcactatct
    10261 attgactaca agaacgtgat cgcagctcta cgaaaccttt actttatttt agaaatattt
    10321 tttcatgcta ttagcaaaaa aaaagttttt ttttgtctaa gtacacttaa aattttccaa
    10381 aatttttaag atttttcaaa atgtgcgatc aaagatctga caaaatgcca aaattttgaa
    10441 gaggaaattt agtcggcttc caaaattata agtggcagaa attgagcaat tgtcactttt
    10501 tgacagtaaa tagaaaattt tttgaaaaat tttgaaacgc tttattttgt tacttggtca
    10561 ttttggcacc ataggagtag tttttaacat tttccccact ggcgctactt cattttaaac
    10621 ttttccaaag attttaattt ttaattttaa aaaatttcat ataattcgtc tgcgtcttac
    10681 agtttcaggc ttcaaaaatc tattacatat cttatctttg aattcagatg caaaagagaa
    10741 tcgccttgtc gtttccagaa gaagtactcg agcacgtctt ctccttcatc caactcgaca
    10801 aggaccgtaa ctccgtctcc ctcgtctgca agtcctggta cgagatcgag cgttggtgcc
    10861 gtcgtaaggt cttcatcgga aactgctacg ccgtctcccc agccaccgtc atccgtcgtt
    10921 tcccaaaggt ccgttccgtc gagctcaagg gaaagccaca cttcgccgac ttcaacctcg
    10981 tcccagacgg atggggagga tacgtctacc catggatcga ggccatgtcc tcctcctaca
    11041 cctggctcga ggagatccgt ctcaagcgta tggtcgtcac cgacgactgc ctcgagctca
    11101 tcgccaagtc cttcaagaac ttcaaggtcc tcgtcctctc ctcctgcgag ggattctcca
    11161 ccgacggact cgccgccatc gccgccacct gccgtaacct caaggtaagt ttaaacatat
    11221 atatactaac taaccctgat tatttaaatt ttcaggagct cgacctccgt gagtccgacg
    11281 tcgacgaggt ctccggacac tggctctccc acttcccaga cacctacacc tccctcgtct
    11341 ccctcaacat ctcctgcctc gcctccgagg tctccttctc cgccctcgag cgtctcgtca
    11401 cccgttgccc aaacctcaag tccctcaagc tcaaccgtgc cgtcccactc gagaagctcg
    11461 ccaccctcct ccaacgtgcc ccacaactcg aggagctcgg aaccggagga tacaccgccg
    11521 aggtccgtcc agacgtctac tccggactct ccgtcgccct ctccggatgc aaggtaagtt
    11581 taaacagttc ggtactaact aaccatacat atttaaattt tcaggagctc cgttgcctct
    11641 ccggattctg ggacgccgtc ccagcctacc tcccagccgt ctactccgtc tgctcccgtc
    11701 tcaccaccct caacctctcc tacgccaccg tccaatccta cgacctcgtc aagctcctct
    11761 gccaatgccc aaagctccaa cgtctctggg tcctcgacta catcgaggac gccggactcg
    11821 aggtcctcgc ctccacctgc aaggacctcc gtgagctccg tgtcttccca tccgagccat
    11881 tcgtcatgga gccaaacgtc gccctcaccg agcaaggact cgtctccgtc tccatgggat
    11941 gcccaaagct cgagtccgtc ctctacttct gccgtcaaat gaccaacgcc gccctcatca
    12001 ccatcgcccg taaccgtcca aacatgaccc gtttccgtct ctgcatcatc gagccaaagg
    12061 ccccagacta cctcaccctc gagccactcg acatcggatt cggagccatc gtcgagcact
    12121 gcaaggacct ccgtcgtctc tccctctccg gactcctcac cgacaaggtc ttcgagtaca
    12181 tcggaaccta cgccaagaag atggagatgc tctccgtcgc cttcgccgga gactccgacc
    12241 tcggactcca ccacgtcctc tccggatgcg actccctccg taagctcgag atccgtgact
    12301 gcccattcgg agacaaggcc ctcctcgcca acgcctccaa gctcgagacc atgcgttccc
    12361 tctggatgtc ctcctgctcc gtctccttcg gagcctgcaa gctcctcgga caaaagatgc
    12421 caaagctcaa cgtcgaggtc atcgacgagc gtggagcccc agactcccgt ccagagtcct
    12481 gcccagtcga gcgtgtcttc atctaccgta ccgtcgccgg accacgtttc gacatgccag
    12541 gattcgtctg gaacatggac caagactcca ccatgcgttt ctcccgtcaa atcatcacca
    12601 ccaacggact cggaggagga ggatccccag tcaagcaaac cctcaacttc gacctcctca
    12661 agctcgccgg agacgtcgag tccaacccag gaccaatggt ttcggaattg attaaggaaa
    12721 atatgcacat gaagctctac atggagggaa ccgtcgacaa ccaccacttc aagtgcacct
    12781 ccgagggaga gggaaagcca tacgagggaa cccaaaccat gcgtatcaag gtcgtcgagg
    12841 gaggaccact cccattcgcc ttcgacatcc tcgccacctc cttcctctac ggatccaagg
    12901 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag accttcatca
    12961 accacaccca aggaatccca gacttcttca agcaatcctt cccagaggga ttcacctggg
    13021 agcgtgtcac cacctacgag gacggaggag tcctcaccgc cacccaagac acctccctcc
    13081 aagacggatg cctcatctac aacgtcaagg taagtttaaa cagttcggta ctaactaacc
    13141 atacatattt aaattttcag atccgtggag tcaacttcac ctccaacgga ccagtcatgc
    13201 aaaagaagac cctcggatgg gaggccttca ccgagaccct ctacccagcc gacggaggac
    13261 tcgagggacg taacgacatg gccctcaagc tcgtcggagg atcccacctc atcgccaacg
    13321 ccaaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagacca
    13381 cctaccgttc caagaagcca gccaagaacc tcaagatgcc aggagtctac tacgtcgact
    13441 accgtctcga gcgtatcaag gaggccaaca acgagaccta cgtcgagcaa cacgaggtcg
    13501 ccgtcgcccg ttactgcgac ctcccatcca agctcggaca caagctcaac cctaaagatc
    13561 cagccaaacc tccggccaag gcacaagttg tgggatggcc accggtgaga tcataccgga
    13621 agaacgtgat ggtttcctgc caaaaatcaa gcggtggccc ggaggcggcg gcgttcgtga
    13681 aaccagccgc caagcgtgtc aagctcgact aagataaatg caaaatcctt tcaagcattc
    13741 ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta atttatttgc
    13801 ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc atctgagtga
    13861 agtgaatgct atcaaaatgt gattctgtct gatgtacttt cacaatctct cttcaattcc
    13921 attttgaagt gctttaaacc cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat
    13981 tgtgttcgtt gagtgaccca acaaaaagag gaaactttat tgtgccgcca agaaaaaagt
    14041 ctcacgtacg gtgcgcgcga tgccctaggc atggtcatag ctgtttcctg cgcaagtcgg
    14101 cgatttcttt gaagttttgt caaaagaaga gacaaggact tgatagatac gctcatcaga
    14161 gtgagattca ttgcaaatag tgtgtaaatc gtggagagac gagagaaaag gtgtgagaag
    14221 cttagaagat aaagaatagg aagaagaaca tatctcattt cctattttct ctaatttttc
    14281 gttcattttt ctagacatta cccatttgat ggtttttcaa tatggttatc agggtactca
    14341 ttttggtagt tttcaaagca atttggagtt tttttttaaa ttgtcaacta caaaaacgca
    14401 gcttaaaggt ccaaatatca tagtataaaa attctttaaa aaattccaaa ttttatatga
    14461 ttttttgaaa attggaagaa tgttagtttt tgccattttt ttggaagttt ttctccggat
    14521 tttatggtac ttatatttca aatataaatt tttgtaggaa ttcgagtatg cgagagagct
    14581 atagtttact aaaaaaaagt ggcaaaaact cagtaattgc cacttttttc aaaaatgttt
    14641 tgaaaaattt tactatgata tttggccatt ttggcaccat atgtacgtta ctttttacga
    14701 aacactctaa tttttgaaat ttgagtttca tgtttaatct tttgtataaa atttttatgg
    14761 tccttttgtg acaggataaa aaattgcaaa aaaatagaaa actttgtttc gtgaaaacac
    14821 ctagtttaaa aattgtgggc cacttagcga ttctagtcaa aaatcagaca aatgtcagct
    14881 gccgtaactt tttccggatc agggttatca aattttaatt ttaattgtaa atgtagaaaa
    14941 atgatcggca acattttgct aattgaaaat attttgaaaa ttgctatagt acatattaat
    15001 ctagtattaa tatagtatta atgttataat atcagttatg tgtttgaata actcaagcgt
    15061 aagtttccgt aaaatatctt taaattttgg taatttttta cggtttggta taaatttttg
    15121 cattttttga ttttctacac tttttttcga tttcgcattt tccgaatttc tctgaaataa
    15181 atcgaacaat cggaaaattt tcgagggcac ccttagccat gcagctcagg aatttgactt
    15241 tatgttccca ttcaaatttt tttaaaatta aataaaatgc taattgtaag ataaattaca
    15301 aaaattatta aatctcatca tccttatgat gatagtgcag tgcatgaata tatttttcca
    15361 aataggatat gattcgtcga gtagcttcac gtaatccgtt ttcttcaatc accatttcga
    15421 actgcaattt tttttttgga tttttgtaac aatattgtca taaattgtga ttttgatatt
    15481 ttgcgtagag catcagaacc tttctgcttt gaattttaat gggattaaaa attataaaaa
    15541 acttgcaccc taattaaagc gccaacttta ttatacatag ttgataattc gatggtatca
    15601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    15661 atgtgagcaa aagg
//
