LOCUS       sequence-343544-       14647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms
            for insertion in ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14647)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 14647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD74
FEATURES             Location/Qualifiers
     source          1..14647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..26
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(1533..1566)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1566..1577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2999..3322)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5240..5264
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5267..6292
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5267..6292
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6293..6317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6670..6703)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7072..7089
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7073..7089
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9370..9435
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             10291..10422
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             10423..10449
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      10458..10781
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(10814..10830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10814..10830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(12303..12323)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(12460..13048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12540..12559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13219..14010,14011..14079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13842..13861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14080..14184)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14252..14270
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14308..14330)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gctatcaact ttgtatagaa aagttgcaag tggggatcag gaagaagttg ataaagtgat
       61 tcaaaaactc gaggagctca acacagcgat gaacccaatt ttgagagcaa cactgacgaa
      121 gaactatgac gcgagggtag gcactacctt gaagctatcc acctgcctgc ctgccgccta
      181 tttccatgtg cctactgcgc atggaaatag gcggtagtca ggcaggtaga tagcctcata
      241 aaattcaata cttccttttc agaaagaatg ctggaatgta ttcaaagagg agtcactctt
      301 cccattgctc agagaatatg aagagttgct cggcgagcaa aagttcatga ttggagatcg
      361 atattcatgg gctgacatcg ctgtcgtcga gtttctaact cggtgtcagc agtgctacga
      421 tagcttctat ctggctcatt tcgagaggct ccgaactttt tgccagactt ttgagtctct
      481 tccgcacatt cgtccctaca ttcagggacg tgttgattca tttatctaat tttttaaaat
      541 ggatattttt cgttttttat ttgtttgttt tgctgataat tcaataaagt tacgcaacga
      601 attttagaca tttatgaaaa ccgaaaatcg acaaaaatat tttaagaaaa atagcagcaa
      661 aattcaatat tttatcaaat tttgtttatg gtaatgagaa agaaaacata caatattttt
      721 ctaagaagaa gaaaaatgac cagaatttta agatttttct cgaatatacg aaaaattgat
      781 aaactaaaat tttcgatttt tccatttatt ttgaaaaaaa aactcttgac ggttttcacc
      841 atgcgaagag gtgagaaata ttctgaaagt tatgcatttt gaaaaaattg caaaaaaata
      901 cgttaaaatt ttttgatcta ttgttttttt ttgaaaaccg gaatttaaaa tggaattttt
      961 tttcaaaaat taaaattaaa atttaaatat ttctagtgtt tggatagttt ttcaaaagct
     1021 agatttaacc cgcgagatgt cgaccgctag tgtagcttac agatggctga agacttcgca
     1081 aaaatcttat cttagaaacc cgcgaggtgt cggccgctaa aactttgtaa acctgccaac
     1141 aaaatgcttc gcattgtctg aacagatgaa accttgaaac aaaacatata caattgaaat
     1201 cccgtaaagt gtcgggcgca agaactgcga ttagttgtat aattggacta gctcaaattt
     1261 gaccaaaatc tcatttttag acctctaaga tgtcggccac tataacttta ttcaacttga
     1321 aactgcaaaa aaacttatca actgtataga ttcaagtatt atgtcaagta ttggttttag
     1381 agctacagta cactctgata acaaaatctg cagaaatacc tccctgtcaa ttcccaaaat
     1441 acctcccctc atctcaatta tcccgaaaat cggttagtca ttgccatcag aaaatcgaat
     1501 taggtaccat gatggtagca aactcacttc gtataacttc gtatagtata ccttatacga
     1561 agttatctgc cctcgattgt ctatcttcca ttctcaccaa gtttcaacaa ctttattcag
     1621 tcattcttct ctggttcatt tcccaaccaa tcttcccgaa taaagcccat ctgtcctttc
     1681 ggccacattc ctcacctaca tccgtactcc ttatctcccg ttcttatcca gtcttttatc
     1741 gtctctgcgt ctcgcacact gtctcgtcgg taatgtaaaa aaaaaacaaa aaaaacttga
     1801 aaacttgcgg aaagatttcc taataaaagg agacgaatcc ggtaggaaat cgacaagtct
     1861 ctgcttctcg aagactaggt gagttacatt tctttggccg aattattttg aaatcctaaa
     1921 tttgataatt tcagagatgt ctgtaaaact tgcgtgttat gtgacggctt cggtcactgt
     1981 cgccactctt atggtatgct tcatgaccat gtcaaccatc tactcggagg ttgatggatt
     2041 cagagagaag cttgataccg agatgaatgt gttcagagtg agttaaatta tattttgaat
     2101 tttaaataat ttttaatttt ctagcaatct accaatggat tgtggaagga catagttgtc
     2161 atcggaagat ctagcaagcg tgtccgttgt caatatgaag agaccaacgc taccccaact
     2221 ccacatgctg atggatcccc atctgctcca ccaggtcaac caccagcagt tccaccagtc
     2281 ttcaaccagc caaagactcc aaatggagcc aatggaaatg gaccaacctg caactgcaat
     2341 gctgataaca agtgcccagc tggaccatcc ggaccaaagg gagttccagg agttccagga
     2401 ctcgacggag ttccaggact tgacggtgtt ccaggagttg gagctgatga tatcgctcca
     2461 caacgcgagt ctgtcggatg cttcacttgc ccacaaggac cagttggacc accaggagct
     2521 cttggaagac caggaccacg tggacttcca ggaccaagag gacaaaatgg aaacccagga
     2581 agagatggac aaccaggaca tccaggagag caaggatcat ccggtcaaat cggaaagatc
     2641 ggagagccag gaccaccagg agagaaggga cgcgacgccg agcatccaat cggaagacca
     2701 ggaccaaagg gaccaagagg agatcaagga ccaacaggac cagctggaca gaacggtctt
     2761 cacggaccac caggagagcc aggaaccgtt ggaccagaag gaccatctgg aaagcaagga
     2821 cgtcaaggac cagacggaac ccagggagag actggaccag acggaagacc aggaaaggat
     2881 gccgagtact gccagtgccc agacaagtct ccaccatcag aggctgtcaa cgccaaccgt
     2941 ggatacagaa atatctaaat tgttggtgtt ttctaataaa atatttgaga ttcagtgaga
     3001 cttttttctt ggcggcacaa taaagtttcc tctttttgtt gggtcactca acgaacacaa
     3061 tacaaatatt tgagcgctcg catttttctc aacctttcgg gtttaaagca cttcaaaatg
     3121 gaattgaaga gagattgtga aagtacatca gacagaatca cattttgata gcattcactt
     3181 cactcagatg caaacttttc agtgactata aaaagtcata aaataataac attaaaaaag
     3241 caaataaatt agcgagagaa ttttttgaca aaaagaaaga atagtgatag agaagaaggg
     3301 aatgcttgaa aggattttgc atttatctta atcaccgtct tcgagaagac gaaccatggc
     3361 gccagtttca gaatccaagt ttctaatata gttcatcaca atgttgacgt tggtccaacc
     3421 tccggcttgc ataatctctg ggatggaaac tccagcgcga gccatgtcac gggcagctcc
     3481 gacacgggcg gaatgtccag accaagccaa gtaacgttgt ccggaatcat ccttggctcc
     3541 gtagatgaga cggtgggtag cttcgaagat tcctgaaaat ttattttcag cttattctta
     3601 gctaatatgt tgtttaaact taccttcaag agctcgagtc gagagttgtg atgttgcact
     3661 cggggcagca actccgttct tgcgaacacg acagaacagg tagttgtttg gatcatcggc
     3721 gactccggaa accgaaatcc agcgctcaac gagcttggta actccaaggg agagcgcttt
     3781 ttcgactccg gcagtcgaga ccagcgtttt tgtacgtccg atgtggatga gcattcttcc
     3841 tccgtctgtg cgactaatat ccttgacgcg aatgcgggca atttcggcga tacggagaag
     3901 tgtgttgtaa gcaattccga ggaaggcgag gtttcggatg tcctggcaac gatcggaatt
     3961 ttccataaga gaacggacct gatcgaagtc ggttctgaaa atttcgtcaa gtcttaggat
     4021 tgaatacatt aatatttaaa cttacctttc gaaggcgagg gcttgcttgg ctctctctcc
     4081 agcatcaaca ttttccttgc ggatacgtct cattacgaga gatacggcat tagaatctga
     4141 tgggcgtgga agtccagatc ggcgatggag catattgagc tgtccgagat gttgttggat
     4201 cgtcttcacc gccaatccac gagcttggag gtagagcaag tagtcacgaa cgtcttctgg
     4261 ctcggctggg aaccacttgc ggttgttaag cttgcaccag gcagcccaag aacggcacac
     4321 tgagagaagc atcttccagg tgtgctcgga gaaggcttgt ctgtcacgga acatatccat
     4381 gaggttcttg cgaacttcat cacttgtcgc gtcaactgga agtgctggaa gattttgatg
     4441 aacagtgaga agatttgaca tattttcgaa gttttttaga tgcactagaa caaagcgtgt
     4501 tggcttcctc tgagcccgct ttccttatat acccgcattc tgcagcctta cagaatgttc
     4561 tagaaggtcc tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag agacgcagac
     4621 ggaaaatgta tctgggtctc tttattgtgt acactacttt tccatgtacc gaatgtgagt
     4681 cgccctcctt ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa atagtataaa
     4741 taccgttgaa aataaatacc gaacaacatt tgctctaatt gtgaaattag aaatcttcaa
     4801 actataatca tgtcacttta ccactatttc cgtccagctc aacgttccgt ttttggtgat
     4861 catttttgct ttcgtcgtaa atctacacac gcgtctcttc cgtgcgagag tccaagccag
     4921 cagccaaatt cgttgactga gtattcaacg tttatacgtt gtcggcaacg agaaatagga
     4981 aaatgcatcg ggaaatgttc ttttttcgat tttttccaag gttttgacaa attttaccac
     5041 gaattttgct atgttttcaa ttaaaaaata tgttattcaa ctgtttctat gaggaaaata
     5101 aggctttgca tgtaattttc ttattcagca taatttttaa ttaatttgaa ttttctgtcc
     5161 taacgtttat tttgttttct tggttatgac tgatctgaaa ttaatttttg aattttaagg
     5221 taatatgtca ggcggtgccg caagtttgta caaaaaagca ggctccatga aaaagcctga
     5281 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     5341 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     5401 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     5461 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     5521 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     5581 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     5641 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     5701 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     5761 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     5821 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     5881 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     5941 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     6001 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     6061 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     6121 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     6181 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     6241 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agacccagct
     6301 ttcttgtaca aagtgggtcc aattactctt caacatccct acatgctctt tctccctgtg
     6361 ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc
     6421 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     6481 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     6541 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac
     6601 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     6661 caatttttaa taacttcgta tagtatacct tatacgaagt tatactagta tatattttta
     6721 tgttttcttt taaaccttcc tccatatttc tgaattgaat gttgttgcat attgtgtgtc
     6781 cttatttcat ttccatcatc cttttgtgtg tttccccctt ccaccaattc cccccacgtc
     6841 ctaatgaaat gaaagcgaga agataaataa acttgcacat tgtgttcaga tgagaggagc
     6901 ggaatacgaa ttgggagacg gaaagagaaa aaaaaatgtt tagatttgga ttgaattaat
     6961 tttttagaaa gcaagaaaaa aaacacaaag agaataaata aataatagga aaaaaaacaa
     7021 atattcagta tttaaaattt gaaaattcaa cgacgttggc gtcgatcatc ctgtaaaacg
     7081 acggccagtg catggaccga ccgtactctc tgaattcatt tttcccgatc ttaccaactc
     7141 ccgatctatc tctatccctg gttttttctt cgtgctccaa tggaattctt gagacttcca
     7201 ctatcttctc tggcaccctc cactacgcgt aggcgtctct cgcttcgtgt attcccggga
     7261 agccgggttc ccgtctctcc cgccgctgcc gctgccgcac acagctttac acctcgtaga
     7321 atccccaaag aggggcgtgg cttgcgggtg ccaacatcct cctgccgagg aagaagcagg
     7381 cactcatcac tcgcatcatc aacctcgact aagggagcac cacattagtt ttgaggtaga
     7441 gaaaccattt gaaagaagcg agaaatcatg caaaagagaa tcgccttgtc gtttccagaa
     7501 gaagtactcg agcacgtctt ctccttcatc caactcgaca aggaccgtaa ctccgtctcc
     7561 ctcgtctgca agtcctggta cgagatcgag cgttggtgcc gtcgtaaggt cttcatcgga
     7621 aactgctacg ccgtctcccc agccaccgtc atccgtcgtt tcccaaaggt ccgttccgtc
     7681 gagctcaagg gaaagccaca cttcgccgac ttcaacctcg tcccagacgg atggggagga
     7741 tacgtctacc catggatcga ggccatgtcc tcctcctaca cctggctcga ggagatccgt
     7801 ctcaagcgta tggtcgtcac cgacgactgc ctcgagctca tcgccaagtc cttcaagaac
     7861 ttcaaggtcc tcgtcctctc ctcctgcgag ggattctcca ccgacggact cgccgccatc
     7921 gccgccacct gccgtaacct caaggtaagt ttaaacatat atatactaac taaccctgat
     7981 tatttaaatt ttcaggagct cgacctccgt gagtccgacg tcgacgaggt ctccggacac
     8041 tggctctccc acttcccaga cacctacacc tccctcgtct ccctcaacat ctcctgcctc
     8101 gcctccgagg tctccttctc cgccctcgag cgtctcgtca cccgttgccc aaacctcaag
     8161 tccctcaagc tcaaccgtgc cgtcccactc gagaagctcg ccaccctcct ccaacgtgcc
     8221 ccacaactcg aggagctcgg aaccggagga tacaccgccg aggtccgtcc agacgtctac
     8281 tccggactct ccgtcgccct ctccggatgc aaggtaagtt taaacagttc ggtactaact
     8341 aaccatacat atttaaattt tcaggagctc cgttgcctct ccggattctg ggacgccgtc
     8401 ccagcctacc tcccagccgt ctactccgtc tgctcccgtc tcaccaccct caacctctcc
     8461 tacgccaccg tccaatccta cgacctcgtc aagctcctct gccaatgccc aaagctccaa
     8521 cgtctctggg tcctcgacta catcgaggac gccggactcg aggtcctcgc ctccacctgc
     8581 aaggacctcc gtgagctccg tgtcttccca tccgagccat tcgtcatgga gccaaacgtc
     8641 gccctcaccg agcaaggact cgtctccgtc tccatgggat gcccaaagct cgagtccgtc
     8701 ctctacttct gccgtcaaat gaccaacgcc gccctcatca ccatcgcccg taaccgtcca
     8761 aacatgaccc gtttccgtct ctgcatcatc gagccaaagg ccccagacta cctcaccctc
     8821 gagccactcg acatcggatt cggagccatc gtcgagcact gcaaggacct ccgtcgtctc
     8881 tccctctccg gactcctcac cgacaaggtc ttcgagtaca tcggaaccta cgccaagaag
     8941 atggagatgc tctccgtcgc cttcgccgga gactccgacc tcggactcca ccacgtcctc
     9001 tccggatgcg actccctccg taagctcgag atccgtgact gcccattcgg agacaaggcc
     9061 ctcctcgcca acgcctccaa gctcgagacc atgcgttccc tctggatgtc ctcctgctcc
     9121 gtctccttcg gagcctgcaa gctcctcgga caaaagatgc caaagctcaa cgtcgaggtc
     9181 atcgacgagc gtggagcccc agactcccgt ccagagtcct gcccagtcga gcgtgtcttc
     9241 atctaccgta ccgtcgccgg accacgtttc gacatgccag gattcgtctg gaacatggac
     9301 caagactcca ccatgcgttt ctcccgtcaa atcatcacca ccaacggact cggaggagga
     9361 ggatccccag tcaagcaaac cctcaacttc gacctcctca agctcgccgg agacgtcgag
     9421 tccaacccag gaccaatggt ttcggaattg attaaggaaa atatgcacat gaagctctac
     9481 atggagggaa ccgtcgacaa ccaccacttc aagtgcacct ccgagggaga gggaaagcca
     9541 tacgagggaa cccaaaccat gcgtatcaag gtcgtcgagg gaggaccact cccattcgcc
     9601 ttcgacatcc tcgccacctc cttcctctac ggatccaagg taagtttaaa catatatata
     9661 ctaactaacc ctgattattt aaattttcag accttcatca accacaccca aggaatccca
     9721 gacttcttca agcaatcctt cccagaggga ttcacctggg agcgtgtcac cacctacgag
     9781 gacggaggag tcctcaccgc cacccaagac acctccctcc aagacggatg cctcatctac
     9841 aacgtcaagg taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag
     9901 atccgtggag tcaacttcac ctccaacgga ccagtcatgc aaaagaagac cctcggatgg
     9961 gaggccttca ccgagaccct ctacccagcc gacggaggac tcgagggacg taacgacatg
    10021 gccctcaagc tcgtcggagg atcccacctc atcgccaacg ccaaggtaag tttaaacatg
    10081 attttactaa ctaactaatc tgatttaaat tttcagacca cctaccgttc caagaagcca
    10141 gccaagaacc tcaagatgcc aggagtctac tacgtcgact accgtctcga gcgtatcaag
    10201 gaggccaaca acgagaccta cgtcgagcaa cacgaggtcg ccgtcgcccg ttactgcgac
    10261 ctcccatcca agctcggaca caagctcaac cctaaagatc cagccaaacc tccggccaag
    10321 gcacaagttg tgggatggcc accggtgaga tcataccgga agaacgtgat ggtttcctgc
    10381 caaaaatcaa gcggtggccc ggaggcggcg gcgttcgtga aaccagccgc caagcgtgtc
    10441 aagctcgact aagataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt
    10501 ctttcttttt gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt
    10561 atgacttttt atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt
    10621 gattctgtct gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc
    10681 cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca
    10741 acaaaaagag gaaactttat tgtgccgcca agaaaaaagt ctcacgtacg gtgcgcgcga
    10801 tgccctaggc atggtcatag ctgtttcctg cgcaagtcgg cgatttcttt gaagttttgt
    10861 caaaagaaga gacaaggact tgatagatac gctcatcaga gtgagattca ttgcaaatag
    10921 tgtgtaaatc gtggagagac gagagaaaag gtgtgagaag cttagaagat aaagaatagg
    10981 aagaagaaca tatctcattt cctattttct ctaatttttc gttcattttt ctagacatta
    11041 cccatttgat ggtttttcaa tatggttatc agggtactca ttttggtagt tttcaaagca
    11101 atttggagtt tttttttaaa ttgtcaacta caaaaacgca gcttaaaggt ccaaatatca
    11161 tagtataaaa attctttaaa aaattccaaa ttttatatga ttttttgaaa attggaagaa
    11221 tgttagtttt tgccattttt ttggaagttt ttctccggat tttatggtac ttatatttca
    11281 aatataaatt tttgtaggaa ttcgagtatg cgagagagct atagtttact aaaaaaaagt
    11341 ggcaaaaact cagtaattgc cacttttttc aaaaatgttt tgaaaaattt tactatgata
    11401 tttggccatt ttggcaccat atgtacgtta ctttttacga aacactctaa tttttgaaat
    11461 ttgagtttca tgtttaatct tttgtataaa atttttatgg tccttttgtg acaggataaa
    11521 aaattgcaaa aaaatagaaa actttgtttc gtgaaaacac ctagtttaaa aattgtgggc
    11581 cacttagcga ttctagtcaa aaatcagaca aatgtcagct gccgtaactt tttccggatc
    11641 agggttatca aattttaatt ttaattgtaa atgtagaaaa atgatcggca acattttgct
    11701 aattgaaaat attttgaaaa ttgctatagt acatattaat ctagtattaa tatagtatta
    11761 atgttataat atcagttatg tgtttgaata actcaagcgt aagtttccgt aaaatatctt
    11821 taaattttgg taatttttta cggtttggta taaatttttg cattttttga ttttctacac
    11881 tttttttcga tttcgcattt tccgaatttc tctgaaataa atcgaacaat cggaaaattt
    11941 tcgagggcac ccttagccat gcagctcagg aatttgactt tatgttccca ttcaaatttt
    12001 tttaaaatta aataaaatgc taattgtaag ataaattaca aaaattatta aatctcatca
    12061 tccttatgat gatagtgcag tgcatgaata tatttttcca aataggatat gattcgtcga
    12121 gtagcttcac gtaatccgtt ttcttcaatc accatttcga actgcaattt tttttttgga
    12181 tttttgtaac aatattgtca taaattgtga ttttgatatt ttgcgtagag catcagaacc
    12241 tttctgcttt gaattttaat gggattaaaa attataaaaa acttgcaccc taattaaagc
    12301 gccaacttta ttatacatag ttgataattc gatggtatca gctcactcaa aggcggtaat
    12361 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    12421 aaagaccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    12481 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    12541 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    12601 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    12661 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    12721 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    12781 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    12841 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    12901 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    12961 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    13021 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    13081 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    13141 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    13201 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    13261 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    13321 gggcttacca tctgacccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    13381 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    13441 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    13501 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    13561 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    13621 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    13681 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    13741 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    13801 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    13861 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    13921 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    13981 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    14041 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    14101 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    14161 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    14221 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    14281 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    14341 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    14401 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgcac tgagagtgca
    14461 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    14521 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    14581 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggat
    14641 ccgccaa
//
