LOCUS       sequence-343545-       13993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant
            Selection: PvUbi2:bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13993)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_26K
FEATURES             Location/Qualifiers
     source          1..13993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..677
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     614..634
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             716..1267
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    1296..1470
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1548..1572
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1997..2791
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2878..3466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3367..3386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3620..3637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3652..3792
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3718..3737)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3878..3900
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4136..4330
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4396..5469)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5898..6527)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7828..7852
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(7873..8121)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8135..8722)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    8908..8929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8944..8949,8950..8967,8968..8974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8982..8998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8987..9009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9006..9022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9006..9022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9018..9425
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     9041..9061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9043..9061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9108..9127)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9109..9127)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9137..9154)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9137..9153)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9146..9168)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(9753..9773)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9780..9845)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(9849..9905)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10979..11284
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11163..11182
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(11723..11743)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11750..11815)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
      301 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
      361 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
      421 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
      481 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      541 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      601 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      661 tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc ctaggatgag
      721 cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc cggcggtctg
      781 caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg agccgcagga
      841 accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct ggctcgtcgc
      901 cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggt ccctggaagg cacgcaacgc
      961 ctacgactgg acggccgagt ccaccgtgta cgtcagcccc cgccaccagc ggacgggact
     1021 gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct tcaagagcgt
     1081 ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata
     1141 tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc atgacgtggg
     1201 tttctggcag ctggacttca gcctgccggt gccgccccgt ccggtcctgc ccgtcaccga
     1261 aatctgatga ttaattaacg atcgacaagc tctattttct ccataataat gtgtgagtag
     1321 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
     1381 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     1441 aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac
     1501 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     1561 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     1621 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     1681 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     1741 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     1801 cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
     1861 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
     1921 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
     1981 aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
     2041 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
     2101 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
     2161 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
     2221 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
     2281 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
     2341 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
     2401 cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga
     2461 ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt
     2521 aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa
     2581 catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc
     2641 ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga
     2701 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
     2761 atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta
     2821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3001 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     3061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3661 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     3721 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3781 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3841 ctcccggcat ccgcttacag acaagctgtg accgtttccg ggagctgcat gtgtcagagg
     3901 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     3961 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     4021 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     4081 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     4141 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     4201 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     4261 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     4321 gctatccaca ggaaagaggc cttttcgacc tttttcccct gctagggcaa tttgccctag
     4381 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     4441 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     4501 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     4561 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     4621 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     4681 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     4741 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     4801 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     4861 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     4921 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     4981 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     5041 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     5101 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     5161 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     5221 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     5281 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     5341 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     5401 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     5461 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcaggca
     5521 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     5581 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     5641 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     5701 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     5761 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     5821 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     5881 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     5941 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     6001 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     6061 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     6121 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     6181 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     6241 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     6301 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     6361 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     6421 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     6481 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     6541 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     6601 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     6661 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
     6721 ttgagccgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
     6781 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
     6841 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
     6901 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
     6961 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
     7021 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
     7081 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
     7141 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
     7201 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
     7261 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
     7321 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
     7381 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
     7441 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
     7501 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
     7561 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
     7621 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
     7681 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
     7741 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
     7801 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
     7861 tagtttgagc tccttatctt taatcatatt ccatagtcca taccatagca catacagtag
     7921 ttatatgctg cagaagagat ccaacaaaac attcacaatg gattatagaa acatttgttt
     7981 attcattata atgtgatctt acattcattt aatattagaa aaagccacaa attcataaca
     8041 caacaagcca agaaaaaaac acaaacttaa gcacacaagc tttttatttg acacaccaaa
     8101 tatttcatct tcatcttcat atctcgagct attaaaagtt tatctcgccg ttattaaatt
     8161 tccgcctcac ttcctctaag gttaatgtgc cggctttaat catttctcca ccaattaaaa
     8221 gctcttctac actaagaaca gctccattac aattagtgat atgatttaat cgtgtaagct
     8281 gagctttgta gtttccttta aagtgaccac tcacaaataa aaacttaaat tccgttacag
     8341 aagatggata gactttccac cattcattag ggttgagatg tttgtctcgt gtttgatttt
     8401 cttcgacata tcgctccatt tcatctgctt ggccaattgg cagattataa cctccgctat
     8461 aagctttagt atccacgatc acaccgtaat caataggaga tccgacagta taaattgctc
     8521 cgtccggttt ccttgatcca cccaaatgtt tacctctata tccataaact ttcataaaaa
     8581 attccattac cttcatttca agaattctat cctgagtgga atttctggca atttcaatta
     8641 attcaatata ttcatgaggc acatatttca atttatgacg aagttcagat ttcttctcct
     8701 ccagttcact tttgactagt tgagatctgg caacgcgatg ggacgtgcgt tcgccaatac
     8761 ggcgattgac tcttctgatc aattccggcg cgtgcggcaa tccctttttc actgcatcca
     8821 tggcaggacg tccgccgagg cacgcgagtg ccaccagatg atcgttggag accaccggtg
     8881 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8941 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9001 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     9061 ggaacaaaag ctggaggacc tcaaggggcg gcccggtacc caattcgccc tatagtgagt
     9121 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9181 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9241 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     9301 cctgtagcgg cgccggtctc caggggggca cccgtcagtg cattgcgcga tgcatgcact
     9361 gcctccattg cggtcacgcc gccacgtttt gcaatcttca caagttggcc tgtgtccaac
     9421 tgtaacggcg gacctctcaa ctcccccgca tccgtgagca aggcctccag ggcgcgtgcg
     9481 ccggaccact gtttgccgac gccaacgatg tcttcgtgtg tcgcctctgg caacgccgtg
     9541 attatgtgct gatacgtgac agcgacggtc cctaacgctg ccgggtgttg gctgagcgca
     9601 acgatgtgcg cgtgtgtaaa cccatggccc accagtgcct cgtggtgctg cgccactgtc
     9661 gaacgcacct tcggtttgat cttctcttgc tgctgctgac tgtagccgag cgtgcgtaga
     9721 tccaccatgc tagccggcac cccgtgaatg cccaccttcc tcttcttctt gggggccatc
     9781 ttgtcatcgt catccttgta atcgatgtca tgatctttat aatcaccgtc atggtctttg
     9841 tagtccatgg gccctgggtt ttcttccacg tcacctgctt gctttaacag agagaagttc
     9901 gtggcgccgc ttccggacct gaaattgatt tcaccattgt tgaacttgcg gcgcacctct
     9961 tccagggtca gggtaccagc cttgatcatc tcgccgccga tcagcagttc ctccacggac
    10021 agcacggcgc cgttgcagtt ggtcttgcgg ttcaggcggg tcagttgggc cttataattg
    10081 cccttgaagt ggccggacac gaacaggaat ttgaactcgg tcacggagga ggggtacacc
    10141 ttccaccact cgttgggatt aatgtgctta ttgcgggtct ggttctcctt cacgtagcgt
    10201 tgcatctcgt cggcctgacc gatgggtaag ttgtagccgc cggagtaggc cttggtgtcg
    10261 acaatgacgc catagtcgat gggggagccc acggtgtaga tggcgccatc gggcttgcgg
    10321 gagccgccca ggtgcttgcc gcggtagccg tacaccttca tgaagaactc catcactttc
    10381 atctccagga tgcggtcttg ggtgctgttg cgggcgatct cgatcagctc gatgtactcg
    10441 tggggcacgt acttgagctt gtggcgcagc tcgctctttt tttcttccag ctcggacttc
    10501 accagctggg atccggcaac gcgatgggac gtgcgttcgc caatacggcg attgactctt
    10561 ctgatcaatt ccggcgcgtg cggcaatccc tttttcactg catccatggc aggacgtccg
    10621 ccgaggcagg ccaaggcgac gaggtggtcg ttggagacga agccagataa cagtatgcgt
    10681 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
    10741 caaaaggagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
    10801 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
    10861 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
    10921 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
    10981 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
    11041 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
    11101 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
    11161 gcgcatgatg accaccgata tggccagtgt gccggtttcc gttatcgggg aagaagtggc
    11221 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
    11281 ataacgtctc caggggggca cctgttaacg cattcctgct ggcatgcact gcctccattg
    11341 cggtcacgcc gccacgtttt gcaatcttca caagttggcc tgtgtccaac tgtaacggcg
    11401 gacctctcaa ctcccccgca tccgtgagca aggcctccag ggcgcgtgcg ccggaccact
    11461 gtttgccgac gccaacgatg tcttcgtgtg tcgcctctgg caacgccgtg attatgtgct
    11521 gatacgtgac agcgacggtc cctaacgctg ccgggtgttg gctgagcgca acgatgtgcg
    11581 cgtgtgtaaa cccatggccc accagtgcct cgtggtgctg cgccactgtc gaacgcacct
    11641 tcggtttgat cttctcttgc tgctgctgac tgtagccgag cgtgcgtaga tccaccattc
    11701 tagagggaac gccatggata ccgactttgc gctttttctt aggagccatt ttatcgtcat
    11761 cgtctttgta gtcaatatcg tggtccttgt agtcgccatc gtgatcctta taatccatgc
    11821 ttccccctct ttttcaaaga acaagaaacc actccgaaga gatcgaaagc tcaggttttc
    11881 ttttttcttg aatcactcgc agttcgttgc ttggaattcc ctgcaggaag taacaccaaa
    11941 caacagggtg agcatcgaca aaagaaacag taccaagcaa ataaatagcg tatgaaggca
    12001 gggctaaaaa aatccacata tagctgctgc atatgccatc atccaagtat atcaagatca
    12061 aaataattat aaaacatact tgtttattat aatagatagg tactcaaggt tagagcatat
    12121 gaatagatgc tgcatatgcc atcatgtata tgcatcagta aaacccacat caacatgtat
    12181 acctatccta gatcgatatt tccatccatc ttaaactcgt aactatgaag atgtatgaca
    12241 cacacataca gttccaaaat taataaatac accaggtagt ttgaaacagt attctactcc
    12301 gatctagaac gaatgaacga ccgcccaacc acaccacatc atcacaacca agcgaacaaa
    12361 aagcatctct gtatatgcat cagtaaaacc cgcatcaaca tgtataccta tcctagatcg
    12421 atatttccat ccatcatctt caattcgtaa ctatgaatat gtatggcaca cacatacaga
    12481 tccaaaatta ataaatccac caggtagttt gaaacagaat tctactccga tctagaacga
    12541 ccgcccaacc agaccacatc atcacaacca agacaaaaaa aagcatgaaa agatgacccg
    12601 acaaacaagt gcacggcata tattgaaata aaggaaaagg gcaaaccaaa ccctatgcaa
    12661 cgaaacaaaa aaaatcatga aatcgatccc gtctgcggaa cggctagagc catcccagga
    12721 ttccccaaag agaaacactg gcaagttagc aatcagaacg tgtctgacgt acaggtcgca
    12781 tccgtgtacg aacgctagca gcacggatct aacacaaaca cggatctaac acaaacatga
    12841 acagaagtag aactaccggg ccctaaccat ggaccggaac gccgatctag agaaggtaga
    12901 gagggggggg gggggaggac gagcggcgta ccttgaagcg gaggtgccga cgggtggatt
    12961 tgggggagat ctggttgtgt gtgtgtgcgc tccgaacaac acgaggttgg ggaaagaggg
    13021 tgtggagggg gtgtctattt attacggcgg gcgaggaagg gaaagcgaag gagcggtggg
    13081 aaaggaatcc cccgtagctg ccggtgccgt gagaggagga ggaggccgcc tgccgtgccg
    13141 gctcacgtct gccgctccgc cacgcaattt ctggatgccg acagcggagc aagtccaacg
    13201 gtggagcgga actctcgaga ggggtccaga ggcagcgaca gagatgccgt gccgtctgct
    13261 tcgcttggcc cgacgcgacg ctgctggttc gctggttggt gtccgttaga ctcgtcgacg
    13321 gcgtttaaca ggctggcatt atctactcga aacaagaaaa atgtttcctt agttttttta
    13381 atttcttaaa gggtatttgt ttaattttta gtcactttat tttattctat tttatatcta
    13441 aattattaaa taaaaaaact aaaatagagt tttagttttc ttaatttaga ggctaaaata
    13501 gaataaaata gatgtactaa aaaaattagt ctataaaaac cattaaccct aaaccctaaa
    13561 tggatgtact aataaaatgg atgaagtatt atataggtga agctatttgc aaaaaaaaag
    13621 gagaacacat gcacactaaa aagataaaac tgtagagtcc tgttgtcaaa atactcaatt
    13681 gtcctttaga ccatgtctaa ctgttcattt atatgattct ctaaaacact gatattattg
    13741 tagtactata gattatatta ttcgtagagt aaagtttaaa tatatgtata aagatagata
    13801 aactgcactt caaacaagtg tgacaaaaaa aatatgtggt aattttttat aacttagaca
    13861 tgcaatgctc attatctcta gagaggggca cgaccgggtc acgctgcact gcaggcgcgc
    13921 ccatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
    13981 accaaagggc tat
//
