LOCUS       sequence-343548-       15421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi4348 site on LGI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15421)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 15421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD75
FEATURES             Location/Qualifiers
     source          1..15421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1507..1540)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1540..1551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2973..3296)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5214..5238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5241..6266
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5241..6266
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6267..6291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6644..6677)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7046..7063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7047..7063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10118..10183
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             11039..11170
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             11171..11197
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      11206..11529
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(11562..11578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11562..11578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(13051..13071)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(13208..13796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13288..13307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13967..14758,14759..14827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14590..14609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14828..14932)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15000..15018
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15056..15078)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    15401..15421
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcctact gcgcatggaa
      181 ataggcggta gtcaggcagg tagatagcct cataaaattc aatacttcct tttcagaaag
      241 aatgctggaa tgtattcaaa gaggagtcac tcttcccatt gctcagagaa tatgaagagt
      301 tgctcggcga gcaaaagttc atgattggag atcgatattc atgggctgac atcgctgtcg
      361 tcgagtttct aactcggtgt cagcagtgct acgatagctt ctatctggct catttcgaga
      421 ggctccgaac tttttgccag acttttgagt ctcttccgca cattcgtccc tacattcagg
      481 gacgtgttga ttcatttatc taatttttta aaatggatat ttttcgtttt ttatttgttt
      541 gttttgctga taattcaata aagttacgca acgaatttta gacatttatg aaaaccgaaa
      601 atcgacaaaa atattttaag aaaaatagca gcaaaattca atattttatc aaattttgtt
      661 tatggtaatg agaaagaaaa catacaatat ttttctaaga agaagaaaaa tgaccagaat
      721 tttaagattt ttctcgaata tacgaaaaat tgataaacta aaattttcga tttttccatt
      781 tattttgaaa aaaaaactct tgacggtttt caccatgcga agaggtgaga aatattctga
      841 aagttatgca ttttgaaaaa attgcaaaaa aatacgttaa aattttttga tctattgttt
      901 ttttttgaaa accggaattt aaaatggaat tttttttcaa aaattaaaat taaaatttaa
      961 atatttctag tgtttggata gtttttcaaa agctagattt aacccgcgag atgtcgaccg
     1021 ctagtgtagc ttacagatgg ctgaagactt cgcaaaaatc ttatcttaga aacccgcgag
     1081 gtgtcggccg ctaaaacttt gtaaacctgc caacaaaatg cttcgcattg tctgaacaga
     1141 tgaaaccttg aaacaaaaca tatacaattg aaatcccgta aagtgtcggg cgcaagaact
     1201 gcgattagtt gtataattgg actagctcaa atttgaccaa aatctcattt ttagacctct
     1261 aagatgtcgg ccactataac tttattcaac ttgaaactgc aaaaaaactt atcaactgta
     1321 tagattcaag tattatgtca agtattggtt ttagagctac agtacactct gataacaaaa
     1381 tctgcagaaa tacctccctg tcaattccca aaatacctcc cctcatctca attatcccga
     1441 aaatcggtta gtcattgcca tcagaaaatc gaattaggta ccatgatggt agcaaactca
     1501 cttcgtataa cttcgtatag tataccttat acgaagttat ctgccctcga ttgtctatct
     1561 tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt catttcccaa
     1621 ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc tacatccgta
     1681 ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca cactgtctcg
     1741 tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa
     1801 aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact aggtgagtta
     1861 catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag atgtctgtaa
     1921 aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta tgcttcatga
     1981 ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat accgagatga
     2041 atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa ttttctagca
     2101 atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca agcgtgtccg
     2161 ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat ccccatctgc
     2221 tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga ctccaaatgg
     2281 agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc cagctggacc
     2341 atccggacca aagggagttc caggagttcc aggactcgac ggagttccag gacttgacgg
     2401 tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg gatgcttcac
     2461 ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac cacgtggact
     2521 tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag gacatccagg
     2581 agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac caggagagaa
     2641 gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa gaggagatca
     2701 aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag agccaggaac
     2761 cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg gaacccaggg
     2821 agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt gcccagacaa
     2881 gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct aaattgttgg
     2941 tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc acaataaagt
     3001 ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt
     3061 tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac
     3121 atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac
     3181 tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt
     3241 gacaaaaaga aagaatagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat
     3301 cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc aagtttctaa
     3361 tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc tctgggatgg
     3421 aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt ccagaccaag
     3481 ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg gtagcttcga
     3541 agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta aacttacctt
     3601 caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg ttcttgcgaa
     3661 cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa atccagcgct
     3721 caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc gagaccagcg
     3781 tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta atatccttga
     3841 cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg
     3901 cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg acctgatcga
     3961 agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt taaacttacc
     4021 tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc ttgcggatac
     4081 gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca gatcggcgat
     4141 ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat ccacgagctt
     4201 ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt
     4261 taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc caggtgtgct
     4321 cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact tcatcacttg
     4381 tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt gacatatttt
     4441 cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc cgctttcctt
     4501 atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg cattcgtttg
     4561 aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg tctctttatt
     4621 gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa caagcagctc
     4681 gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa taccgaacaa
     4741 catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac tttaccacta
     4801 tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac
     4861 acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga ctgagtattc
     4921 aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat gttctttttt
     4981 cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt tcaattaaaa
     5041 aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat tttcttattc
     5101 agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt ttcttggtta
     5161 tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt
     5221 tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5281 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5341 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5401 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5461 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5521 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5581 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5641 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5701 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5761 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5821 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5881 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     5941 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6001 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6061 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6121 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6181 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6241 gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg gtccaattac
     6301 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     6361 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     6421 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     6481 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     6541 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     6601 accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt cgtatagtat
     6661 accttatacg aagttatact agtatatatt tttatgtttt cttttaaacc ttcctccata
     6721 tttctgaatt gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg
     6781 tgtgtttccc ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa
     6841 ataaacttgc acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga
     6901 gaaaaaaaaa tgtttagatt tggattgaat taatttttta gaaagcaaga aaaaaaacac
     6961 aaagagaata aataaataat aggaaaaaaa acaaatattc agtatttaaa atttgaaaat
     7021 tcaacgacgt tggcgtcgat catcctgtaa aacgacggcc agtgcatgga tcttcatccc
     7081 ttcaacattt ggcagaaaag tgcaattcgt cttttacagg aattcgattt ccgatctctt
     7141 gcacaactcg aggcgtcact ccagatggat taggaactgt ttccctttgt agtacatgtt
     7201 gggtgtggcg acgtcttccg agtacctact atcctgcata tcttaacgaa gtcgtgtgcg
     7261 attatgcgga cacatcgtgt ctgagtggta gtgaaaagac agagaaagtg ggggaaaaag
     7321 aaaataccat tttttcagga tacgcgtcat gccaaactgg aacccaacag ttgaatgtgc
     7381 tacgaaatga cagcgggaaa ttgattccaa tatcggtatc agctggaatc aattgtgaat
     7441 gtagattagc agttggatca acacttgaat cactcgtact tggacaagga atttccaaag
     7501 caatgccgcc aattgacaca actagtacaa aaccaccaaa tttggcaaca agcacaacaa
     7561 gccattccta atttatttgg gtattttact cttgataatt gtattaatga tcatttaatg
     7621 ttgttgaata tcacacattt aacatattca atcgaagaaa tataacgacg ttccacaaca
     7681 tccagtatac gtcttcctct gtattgagtt ttattctttg acattgtgtc atgtttcaga
     7741 acatagaaat ataccaaagt gttgagaagc attgtataat tcaattgaat ctttttcagt
     7801 ttacctcttc tcctttttca ccgtttccgt catcaaaacg ccgttattca aggtgagatt
     7861 atggcgataa atgaatgcca ttcacaaaat tgcaagacac tattaaagaa gaagaggttt
     7921 agagatttgt accgatcata gcttcttctg agaaagcgac cttggcggcc gtacttgaag
     7981 agaagcaatt tttatcgccg tagaagatgt tgcctgccac ccaccctgta cgttgttttt
     8041 ttgcttatca acatctgaat tttgttgtct tttgcaccgc ctacttttac tatggaattt
     8101 gaggaaatat aatatgaatt gaagagaata cgctacctgt caaagaaacg tcacggttag
     8161 ggtagtataa aagggaaggg taacactgaa taaggtacct ggcttacttt cagtcatgca
     8221 aaagagaatc gccttgtcgt ttccagaaga agtactcgag cacgtcttct ccttcatcca
     8281 actcgacaag gaccgtaact ccgtctccct cgtctgcaag tcctggtacg agatcgagcg
     8341 ttggtgccgt cgtaaggtct tcatcggaaa ctgctacgcc gtctccccag ccaccgtcat
     8401 ccgtcgtttc ccaaaggtcc gttccgtcga gctcaaggga aagccacact tcgccgactt
     8461 caacctcgtc ccagacggat ggggaggata cgtctaccca tggatcgagg ccatgtcctc
     8521 ctcctacacc tggctcgagg agatccgtct caagcgtatg gtcgtcaccg acgactgcct
     8581 cgagctcatc gccaagtcct tcaagaactt caaggtcctc gtcctctcct cctgcgaggg
     8641 attctccacc gacggactcg ccgccatcgc cgccacctgc cgtaacctca aggtaagttt
     8701 aaacatatat atactaacta accctgatta tttaaatttt caggagctcg acctccgtga
     8761 gtccgacgtc gacgaggtct ccggacactg gctctcccac ttcccagaca cctacacctc
     8821 cctcgtctcc ctcaacatct cctgcctcgc ctccgaggtc tccttctccg ccctcgagcg
     8881 tctcgtcacc cgttgcccaa acctcaagtc cctcaagctc aaccgtgccg tcccactcga
     8941 gaagctcgcc accctcctcc aacgtgcccc acaactcgag gagctcggaa ccggaggata
     9001 caccgccgag gtccgtccag acgtctactc cggactctcc gtcgccctct ccggatgcaa
     9061 ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggagctccg
     9121 ttgcctctcc ggattctggg acgccgtccc agcctacctc ccagccgtct actccgtctg
     9181 ctcccgtctc accaccctca acctctccta cgccaccgtc caatcctacg acctcgtcaa
     9241 gctcctctgc caatgcccaa agctccaacg tctctgggtc ctcgactaca tcgaggacgc
     9301 cggactcgag gtcctcgcct ccacctgcaa ggacctccgt gagctccgtg tcttcccatc
     9361 cgagccattc gtcatggagc caaacgtcgc cctcaccgag caaggactcg tctccgtctc
     9421 catgggatgc ccaaagctcg agtccgtcct ctacttctgc cgtcaaatga ccaacgccgc
     9481 cctcatcacc atcgcccgta accgtccaaa catgacccgt ttccgtctct gcatcatcga
     9541 gccaaaggcc ccagactacc tcaccctcga gccactcgac atcggattcg gagccatcgt
     9601 cgagcactgc aaggacctcc gtcgtctctc cctctccgga ctcctcaccg acaaggtctt
     9661 cgagtacatc ggaacctacg ccaagaagat ggagatgctc tccgtcgcct tcgccggaga
     9721 ctccgacctc ggactccacc acgtcctctc cggatgcgac tccctccgta agctcgagat
     9781 ccgtgactgc ccattcggag acaaggccct cctcgccaac gcctccaagc tcgagaccat
     9841 gcgttccctc tggatgtcct cctgctccgt ctccttcgga gcctgcaagc tcctcggaca
     9901 aaagatgcca aagctcaacg tcgaggtcat cgacgagcgt ggagccccag actcccgtcc
     9961 agagtcctgc ccagtcgagc gtgtcttcat ctaccgtacc gtcgccggac cacgtttcga
    10021 catgccagga ttcgtctgga acatggacca agactccacc atgcgtttct cccgtcaaat
    10081 catcaccacc aacggactcg gaggaggagg atccccagtc aagcaaaccc tcaacttcga
    10141 cctcctcaag ctcgccggag acgtcgagtc caacccagga ccaatggttt cggaattgat
    10201 taaggaaaat atgcacatga agctctacat ggagggaacc gtcgacaacc accacttcaa
    10261 gtgcacctcc gagggagagg gaaagccata cgagggaacc caaaccatgc gtatcaaggt
    10321 cgtcgaggga ggaccactcc cattcgcctt cgacatcctc gccacctcct tcctctacgg
    10381 atccaaggta agtttaaaca tatatatact aactaaccct gattatttaa attttcagac
    10441 cttcatcaac cacacccaag gaatcccaga cttcttcaag caatccttcc cagagggatt
    10501 cacctgggag cgtgtcacca cctacgagga cggaggagtc ctcaccgcca cccaagacac
    10561 ctccctccaa gacggatgcc tcatctacaa cgtcaaggta agtttaaaca gttcggtact
    10621 aactaaccat acatatttaa attttcagat ccgtggagtc aacttcacct ccaacggacc
    10681 agtcatgcaa aagaagaccc tcggatggga ggccttcacc gagaccctct acccagccga
    10741 cggaggactc gagggacgta acgacatggc cctcaagctc gtcggaggat cccacctcat
    10801 cgccaacgcc aaggtaagtt taaacatgat tttactaact aactaatctg atttaaattt
    10861 tcagaccacc taccgttcca agaagccagc caagaacctc aagatgccag gagtctacta
    10921 cgtcgactac cgtctcgagc gtatcaagga ggccaacaac gagacctacg tcgagcaaca
    10981 cgaggtcgcc gtcgcccgtt actgcgacct cccatccaag ctcggacaca agctcaaccc
    11041 taaagatcca gccaaacctc cggccaaggc acaagttgtg ggatggccac cggtgagatc
    11101 ataccggaag aacgtgatgg tttcctgcca aaaatcaagc ggtggcccgg aggcggcggc
    11161 gttcgtgaaa ccagccgcca agcgtgtcaa gctcgactaa gataaatgca aaatcctttc
    11221 aagcattccc ttcttctcta tcactcttct ttctttttgt caaaaaattc tctcgctaat
    11281 ttatttgctt ttttaatgtt attattttat gactttttat agtcactgaa aagtttgcat
    11341 ctgagtgaag tgaatgctat caaaatgtga ttctgtctga tgtactttca caatctctct
    11401 tcaattccat tttgaagtgc tttaaacccg aaaggttgag aaaaatgcga gcgctcaaat
    11461 atttgtattg tgttcgttga gtgacccaac aaaaagagga aactttattg tgccgccaag
    11521 aaaaaagtct cacgtacggt gcgcgcgatg ccctaggcat ggtcatagct gtttcctgcg
    11581 caagtcggcg atttctttga agttttgtca aaagaagaga caaggacttg atagatacgc
    11641 tcatcagagt gagattcatt gcaaatagtg tgtaaatcgt ggagagacga gagaaaaggt
    11701 gtgagaagct tagaagataa agaataggaa gaagaacata tctcatttcc tattttctct
    11761 aatttttcgt tcatttttct agacattacc catttgatgg tttttcaata tggttatcag
    11821 ggtactcatt ttggtagttt tcaaagcaat ttggagtttt tttttaaatt gtcaactaca
    11881 aaaacgcagc ttaaaggtcc aaatatcata gtataaaaat tctttaaaaa attccaaatt
    11941 ttatatgatt ttttgaaaat tggaagaatg ttagtttttg ccattttttt ggaagttttt
    12001 ctccggattt tatggtactt atatttcaaa tataaatttt tgtaggaatt cgagtatgcg
    12061 agagagctat agtttactaa aaaaaagtgg caaaaactca gtaattgcca cttttttcaa
    12121 aaatgttttg aaaaatttta ctatgatatt tggccatttt ggcaccatat gtacgttact
    12181 ttttacgaaa cactctaatt tttgaaattt gagtttcatg tttaatcttt tgtataaaat
    12241 ttttatggtc cttttgtgac aggataaaaa attgcaaaaa aatagaaaac tttgtttcgt
    12301 gaaaacacct agtttaaaaa ttgtgggcca cttagcgatt ctagtcaaaa atcagacaaa
    12361 tgtcagctgc cgtaactttt tccggatcag ggttatcaaa ttttaatttt aattgtaaat
    12421 gtagaaaaat gatcggcaac attttgctaa ttgaaaatat tttgaaaatt gctatagtac
    12481 atattaatct agtattaata tagtattaat gttataatat cagttatgtg tttgaataac
    12541 tcaagcgtaa gtttccgtaa aatatcttta aattttggta attttttacg gtttggtata
    12601 aatttttgca ttttttgatt ttctacactt tttttcgatt tcgcattttc cgaatttctc
    12661 tgaaataaat cgaacaatcg gaaaattttc gagggcaccc ttagccatgc agctcaggaa
    12721 tttgacttta tgttcccatt caaatttttt taaaattaaa taaaatgcta attgtaagat
    12781 aaattacaaa aattattaaa tctcatcatc cttatgatga tagtgcagtg catgaatata
    12841 tttttccaaa taggatatga ttcgtcgagt agcttcacgt aatccgtttt cttcaatcac
    12901 catttcgaac tgcaattttt tttttggatt tttgtaacaa tattgtcata aattgtgatt
    12961 ttgatatttt gcgtagagca tcagaacctt tctgctttga attttaatgg gattaaaaat
    13021 tataaaaaac ttgcacccta attaaagcgc caactttatt atacatagtt gataattcga
    13081 tggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    13141 gaaagaacat gtgagcaaaa ggccagcaaa agaccaggaa ccgtaaaaag gccgcgttgc
    13201 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    13261 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    13321 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    13381 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    13441 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    13501 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    13561 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    13621 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    13681 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    13741 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    13801 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    13861 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    13921 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    13981 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    14041 ccgtcgtgta gataactacg atacgggagg gcttaccatc tgaccccagt gctgcaatga
    14101 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    14161 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    14221 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    14281 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    14341 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    14401 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    14461 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    14521 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    14581 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    14641 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    14701 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    14761 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    14821 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    14881 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    14941 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    15001 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    15061 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    15121 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    15181 catcagagca gattgcactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    15241 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    15301 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    15361 ggcgattaag ttgggtaacg ccagggatcc gccaagctat caactttgta tagaaaagtt
    15421 g
//
