LOCUS       sequence-343549-       17613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal promoter rgef-1P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17613)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 17613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD79
FEATURES             Location/Qualifiers
     source          1..17613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1507..1540)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1540..1551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2973..3296)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5214..5238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5241..6266
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5241..6266
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6267..6291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6644..6677)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7046..7063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7047..7063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             12310..12375
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             13231..13362
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             13363..13389
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      13398..13721
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(13754..13770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13754..13770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(15243..15263)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(15400..15988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15480..15499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16159..16950,16951..17019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16782..16801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17020..17124)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     17192..17210
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(17248..17270)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    17593..17613
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcctact gcgcatggaa
      181 ataggcggta gtcaggcagg tagatagcct cataaaattc aatacttcct tttcagaaag
      241 aatgctggaa tgtattcaaa gaggagtcac tcttcccatt gctcagagaa tatgaagagt
      301 tgctcggcga gcaaaagttc atgattggag atcgatattc atgggctgac atcgctgtcg
      361 tcgagtttct aactcggtgt cagcagtgct acgatagctt ctatctggct catttcgaga
      421 ggctccgaac tttttgccag acttttgagt ctcttccgca cattcgtccc tacattcagg
      481 gacgtgttga ttcatttatc taatttttta aaatggatat ttttcgtttt ttatttgttt
      541 gttttgctga taattcaata aagttacgca acgaatttta gacatttatg aaaaccgaaa
      601 atcgacaaaa atattttaag aaaaatagca gcaaaattca atattttatc aaattttgtt
      661 tatggtaatg agaaagaaaa catacaatat ttttctaaga agaagaaaaa tgaccagaat
      721 tttaagattt ttctcgaata tacgaaaaat tgataaacta aaattttcga tttttccatt
      781 tattttgaaa aaaaaactct tgacggtttt caccatgcga agaggtgaga aatattctga
      841 aagttatgca ttttgaaaaa attgcaaaaa aatacgttaa aattttttga tctattgttt
      901 ttttttgaaa accggaattt aaaatggaat tttttttcaa aaattaaaat taaaatttaa
      961 atatttctag tgtttggata gtttttcaaa agctagattt aacccgcgag atgtcgaccg
     1021 ctagtgtagc ttacagatgg ctgaagactt cgcaaaaatc ttatcttaga aacccgcgag
     1081 gtgtcggccg ctaaaacttt gtaaacctgc caacaaaatg cttcgcattg tctgaacaga
     1141 tgaaaccttg aaacaaaaca tatacaattg aaatcccgta aagtgtcggg cgcaagaact
     1201 gcgattagtt gtataattgg actagctcaa atttgaccaa aatctcattt ttagacctct
     1261 aagatgtcgg ccactataac tttattcaac ttgaaactgc aaaaaaactt atcaactgta
     1321 tagattcaag tattatgtca agtattggtt ttagagctac agtacactct gataacaaaa
     1381 tctgcagaaa tacctccctg tcaattccca aaatacctcc cctcatctca attatcccga
     1441 aaatcggtta gtcattgcca tcagaaaatc gaattaggta ccatgatggt agcaaactca
     1501 cttcgtataa cttcgtatag tataccttat acgaagttat ctgccctcga ttgtctatct
     1561 tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt catttcccaa
     1621 ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc tacatccgta
     1681 ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca cactgtctcg
     1741 tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa
     1801 aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact aggtgagtta
     1861 catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag atgtctgtaa
     1921 aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta tgcttcatga
     1981 ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat accgagatga
     2041 atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa ttttctagca
     2101 atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca agcgtgtccg
     2161 ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat ccccatctgc
     2221 tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga ctccaaatgg
     2281 agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc cagctggacc
     2341 atccggacca aagggagttc caggagttcc aggactcgac ggagttccag gacttgacgg
     2401 tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg gatgcttcac
     2461 ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac cacgtggact
     2521 tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag gacatccagg
     2581 agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac caggagagaa
     2641 gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa gaggagatca
     2701 aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag agccaggaac
     2761 cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg gaacccaggg
     2821 agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt gcccagacaa
     2881 gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct aaattgttgg
     2941 tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc acaataaagt
     3001 ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt
     3061 tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac
     3121 atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac
     3181 tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt
     3241 gacaaaaaga aagaatagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat
     3301 cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc aagtttctaa
     3361 tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc tctgggatgg
     3421 aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt ccagaccaag
     3481 ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg gtagcttcga
     3541 agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta aacttacctt
     3601 caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg ttcttgcgaa
     3661 cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa atccagcgct
     3721 caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc gagaccagcg
     3781 tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta atatccttga
     3841 cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg
     3901 cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg acctgatcga
     3961 agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt taaacttacc
     4021 tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc ttgcggatac
     4081 gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca gatcggcgat
     4141 ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat ccacgagctt
     4201 ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt
     4261 taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc caggtgtgct
     4321 cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact tcatcacttg
     4381 tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt gacatatttt
     4441 cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc cgctttcctt
     4501 atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg cattcgtttg
     4561 aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg tctctttatt
     4621 gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa caagcagctc
     4681 gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa taccgaacaa
     4741 catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac tttaccacta
     4801 tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac
     4861 acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga ctgagtattc
     4921 aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat gttctttttt
     4981 cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt tcaattaaaa
     5041 aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat tttcttattc
     5101 agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt ttcttggtta
     5161 tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt
     5221 tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5281 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5341 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5401 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5461 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5521 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5581 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5641 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5701 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5761 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5821 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5881 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     5941 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6001 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6061 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6121 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6181 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6241 gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg gtccaattac
     6301 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     6361 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     6421 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     6481 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     6541 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     6601 accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt cgtatagtat
     6661 accttatacg aagttatact agtatatatt tttatgtttt cttttaaacc ttcctccata
     6721 tttctgaatt gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg
     6781 tgtgtttccc ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa
     6841 ataaacttgc acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga
     6901 gaaaaaaaaa tgtttagatt tggattgaat taatttttta gaaagcaaga aaaaaaacac
     6961 aaagagaata aataaataat aggaaaaaaa acaaatattc agtatttaaa atttgaaaat
     7021 tcaacgacgt tggcgtcgat catcctgtaa aacgacggcc agtgcatgca agactaattt
     7081 tcgattaacc cgtaggggtg caagactaat agagactgca agactattag aggctgaaat
     7141 actaattttc gtatgctcaa taattttgga aattggccta ttttttgtag aaacttgata
     7201 ccgtttaaac aaggaaaaat acacactttt taatatttca tcaataattt gaacgatttt
     7261 gtgattttaa gttcaatttg cccaaaaaaa gacaattttt ctgacgttac cgcaaagtaa
     7321 tgctggtcag gcaaaaagag cggtgcaaaa atataagaga ctgcaatact aatagaggaa
     7381 atacggtaat tgaattttag tgaaacttgc gacagttttc ctcatttttt tgttattccg
     7441 gcatacgagt gtggcatacg agtgaggtca tcttttgttt cttccgtttc ttcatcgctt
     7501 ttgaaaaaaa atgttgaaga aaacttcgta gccgatagcc ggataatgtt tggcacggcg
     7561 ttccaacata tatcattgga aattttttaa gatttttgcg aaaaatcaca ttcttcacga
     7621 tgagaacacg ttattgaagg aatataaatc aagaataaca tatagttata tttctctatt
     7681 actttaacgt taaatatgag caaatttgag cattttgatt gcgatgaaaa gcagaatcga
     7741 gtcaactgaa atccgttcaa aaacctaagc tcggttcgcc cgaaagtcga tttttccaat
     7801 agccgaacag cactgcttct ttattttcca ggtatttttg gggtgtaaga attatatctt
     7861 ctttgtttgg gtatttacgt ttccgaattc cctttcttga tctgtcgcta tttttcagaa
     7921 accagaacag tttatccttt ttcatgagaa cctgaatagc tcaaaaaccc gtttaatttc
     7981 ttttcatcat ctcatttgaa actttcctag tcttcttaat gatttccagg ctcctcctac
     8041 atttgtatct caagatgcat acactttatt tccgctccaa ttcgaatcat ctacttctct
     8101 tttttcttca actttctact tttttccatt cttttctttc aaatgttcga cgtcttcaag
     8161 tgactgctct tcattctcct catttttctc gaatttcatt cttgtctctt ttttgctcaa
     8221 aaatggaaaa tgaaagtgac gtgagatttg gacggcggga cacgggggca gtagaagcag
     8281 caaaaaggag agaaagagga cacaaataag aagaacgaat tcaaaaataa gcggagagga
     8341 gctatttccg tcaattctac ctccccaatc ttcatcaatt cggctcaatt gaatgacgtc
     8401 acagggagat aaaccgtttg gatgagcgcc gtccatcact gacgccatcc cgtttgggac
     8461 aagaaaagag aaaaaagagc acaaagtttt tggtgacgga tcttgtcaat catatgaaag
     8521 ttgttctgat tgattgtcag tttttttcct acttttttgg attctatcca cttctgaact
     8581 tttgacaagt ttcaaacttt ctgaatcatt ttctatgcat tttcctggaa ttctttttat
     8641 gtaaaatatg aaatagaatg tttttgaatt caagttctgc ttttttcttc ttttttgttc
     8701 tgttcgggct tgggtatgct ttttttaaaa attattttgc acatcgacca ataagtgcgc
     8761 aacttataaa attaatttat ttttgttaat ttttgaaata cttgtattgc tttaagtgat
     8821 ctgacctcgc cctgagcttt ccacgtagtt atcaaataca aatcctccaa gggtaacgta
     8881 cctatattac tgatctttat aataacttta tcacctgtcc agttccagag gaattctgtt
     8941 aagcttatag gcacagaagg agtcatttct gctggttttt aatgatccaa atctttattt
     9001 caagtaaaaa actgaacact tgcgaataaa actatcagat taaccattca ccaaaaatgt
     9061 gtttgaatct aaaactttct cagtattcca aatatagaaa taaataacca cgacattgct
     9121 aaaatctgtc tgaattgtgt actccttacc gtgaagtaat aatggatata atgaatcgtt
     9181 tgaaatgaat gatcagcaca tttttggtga aagatcacaa ataaggaata agcgacggaa
     9241 aataaaacga ttatttcgga tcaaaaattt gttgaagatc atatacactc gagaccaaga
     9301 ttattctaga caattttcaa attggcttct ttgtttgcaa atcttcataa taatctgtga
     9361 agtttggaaa tttgaatttt taatcttttt tcatagatta tagtttttat ttctttgcaa
     9421 aactatatta aaaaccgatg cattgtttta gggaaattaa tgagcctttt gttcaacact
     9481 aaaaacaata aaattaaaat tttggcttca tcatttgacc ttttttaagt tcgaaaactt
     9541 ttctcgtatt tctgaaccgc caattttttc acacatctct agacttttgg tgcccgttcc
     9601 agaaagttaa gtaattgcta ttctaagaaa gttctcaaca ttgtttttta gttctgattg
     9661 aattctgatg ttccaggaat atattttaaa tttaatattc tttgcactat ttctatacta
     9721 actaaataat aaatagtcta agtatgttca atgagaacaa aaaagctcta tctatatttt
     9781 tctatcctat ttcatttata tccttttcat tttgaactca ccagcttctc ttcttcttct
     9841 tgtccatata cttcttaaaa gtctcgaact tctctctctc actctccatc aatttcttta
     9901 tggctaccgc ttgcgctctg ccgcttccga agaaagaagg cgttggcaga gctctcaatt
     9961 ctattttttc tcgccgtagg ctatcgattt ctcagcttct cttctctctt cctcactcca
    10021 ccaccaccac ttcgggcttc ttcttttctt cattttcgtc ttcttcttca tcctcttctt
    10081 tttttttcag ttccccctca ctcctcccct tatatgcgcg tgcgagaggg gtgcaaaagc
    10141 agcgcgcatc cgagaattga aacgagaaaa cggagacgca gcagcagttc gtcctcagaa
    10201 aaatagccag taaaaagaga aaaagataga gagagaccta tcgcatttta ttttcaaatt
    10261 gcaattcctg caattcatgt gtgcgtgtgc ccattttcaa ttcttcccgt tatttttcag
    10321 accattacca acgttctttc tagatcttga gacaatcttc cttctgctca atcgttcgtc
    10381 gtgaagacgg catcgacgac gacgacgatg caaaagagaa tcgccttgtc gtttccagaa
    10441 gaagtactcg agcacgtctt ctccttcatc caactcgaca aggaccgtaa ctccgtctcc
    10501 ctcgtctgca agtcctggta cgagatcgag cgttggtgcc gtcgtaaggt cttcatcgga
    10561 aactgctacg ccgtctcccc agccaccgtc atccgtcgtt tcccaaaggt ccgttccgtc
    10621 gagctcaagg gaaagccaca cttcgccgac ttcaacctcg tcccagacgg atggggagga
    10681 tacgtctacc catggatcga ggccatgtcc tcctcctaca cctggctcga ggagatccgt
    10741 ctcaagcgta tggtcgtcac cgacgactgc ctcgagctca tcgccaagtc cttcaagaac
    10801 ttcaaggtcc tcgtcctctc ctcctgcgag ggattctcca ccgacggact cgccgccatc
    10861 gccgccacct gccgtaacct caaggtaagt ttaaacatat atatactaac taaccctgat
    10921 tatttaaatt ttcaggagct cgacctccgt gagtccgacg tcgacgaggt ctccggacac
    10981 tggctctccc acttcccaga cacctacacc tccctcgtct ccctcaacat ctcctgcctc
    11041 gcctccgagg tctccttctc cgccctcgag cgtctcgtca cccgttgccc aaacctcaag
    11101 tccctcaagc tcaaccgtgc cgtcccactc gagaagctcg ccaccctcct ccaacgtgcc
    11161 ccacaactcg aggagctcgg aaccggagga tacaccgccg aggtccgtcc agacgtctac
    11221 tccggactct ccgtcgccct ctccggatgc aaggtaagtt taaacagttc ggtactaact
    11281 aaccatacat atttaaattt tcaggagctc cgttgcctct ccggattctg ggacgccgtc
    11341 ccagcctacc tcccagccgt ctactccgtc tgctcccgtc tcaccaccct caacctctcc
    11401 tacgccaccg tccaatccta cgacctcgtc aagctcctct gccaatgccc aaagctccaa
    11461 cgtctctggg tcctcgacta catcgaggac gccggactcg aggtcctcgc ctccacctgc
    11521 aaggacctcc gtgagctccg tgtcttccca tccgagccat tcgtcatgga gccaaacgtc
    11581 gccctcaccg agcaaggact cgtctccgtc tccatgggat gcccaaagct cgagtccgtc
    11641 ctctacttct gccgtcaaat gaccaacgcc gccctcatca ccatcgcccg taaccgtcca
    11701 aacatgaccc gtttccgtct ctgcatcatc gagccaaagg ccccagacta cctcaccctc
    11761 gagccactcg acatcggatt cggagccatc gtcgagcact gcaaggacct ccgtcgtctc
    11821 tccctctccg gactcctcac cgacaaggtc ttcgagtaca tcggaaccta cgccaagaag
    11881 atggagatgc tctccgtcgc cttcgccgga gactccgacc tcggactcca ccacgtcctc
    11941 tccggatgcg actccctccg taagctcgag atccgtgact gcccattcgg agacaaggcc
    12001 ctcctcgcca acgcctccaa gctcgagacc atgcgttccc tctggatgtc ctcctgctcc
    12061 gtctccttcg gagcctgcaa gctcctcgga caaaagatgc caaagctcaa cgtcgaggtc
    12121 atcgacgagc gtggagcccc agactcccgt ccagagtcct gcccagtcga gcgtgtcttc
    12181 atctaccgta ccgtcgccgg accacgtttc gacatgccag gattcgtctg gaacatggac
    12241 caagactcca ccatgcgttt ctcccgtcaa atcatcacca ccaacggact cggaggagga
    12301 ggatccccag tcaagcaaac cctcaacttc gacctcctca agctcgccgg agacgtcgag
    12361 tccaacccag gaccaatggt ttcggaattg attaaggaaa atatgcacat gaagctctac
    12421 atggagggaa ccgtcgacaa ccaccacttc aagtgcacct ccgagggaga gggaaagcca
    12481 tacgagggaa cccaaaccat gcgtatcaag gtcgtcgagg gaggaccact cccattcgcc
    12541 ttcgacatcc tcgccacctc cttcctctac ggatccaagg taagtttaaa catatatata
    12601 ctaactaacc ctgattattt aaattttcag accttcatca accacaccca aggaatccca
    12661 gacttcttca agcaatcctt cccagaggga ttcacctggg agcgtgtcac cacctacgag
    12721 gacggaggag tcctcaccgc cacccaagac acctccctcc aagacggatg cctcatctac
    12781 aacgtcaagg taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag
    12841 atccgtggag tcaacttcac ctccaacgga ccagtcatgc aaaagaagac cctcggatgg
    12901 gaggccttca ccgagaccct ctacccagcc gacggaggac tcgagggacg taacgacatg
    12961 gccctcaagc tcgtcggagg atcccacctc atcgccaacg ccaaggtaag tttaaacatg
    13021 attttactaa ctaactaatc tgatttaaat tttcagacca cctaccgttc caagaagcca
    13081 gccaagaacc tcaagatgcc aggagtctac tacgtcgact accgtctcga gcgtatcaag
    13141 gaggccaaca acgagaccta cgtcgagcaa cacgaggtcg ccgtcgcccg ttactgcgac
    13201 ctcccatcca agctcggaca caagctcaac cctaaagatc cagccaaacc tccggccaag
    13261 gcacaagttg tgggatggcc accggtgaga tcataccgga agaacgtgat ggtttcctgc
    13321 caaaaatcaa gcggtggccc ggaggcggcg gcgttcgtga aaccagccgc caagcgtgtc
    13381 aagctcgact aagataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt
    13441 ctttcttttt gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt
    13501 atgacttttt atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt
    13561 gattctgtct gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc
    13621 cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca
    13681 acaaaaagag gaaactttat tgtgccgcca agaaaaaagt ctcacgtacg gtgcgcgcga
    13741 tgccctaggc atggtcatag ctgtttcctg cgcaagtcgg cgatttcttt gaagttttgt
    13801 caaaagaaga gacaaggact tgatagatac gctcatcaga gtgagattca ttgcaaatag
    13861 tgtgtaaatc gtggagagac gagagaaaag gtgtgagaag cttagaagat aaagaatagg
    13921 aagaagaaca tatctcattt cctattttct ctaatttttc gttcattttt ctagacatta
    13981 cccatttgat ggtttttcaa tatggttatc agggtactca ttttggtagt tttcaaagca
    14041 atttggagtt tttttttaaa ttgtcaacta caaaaacgca gcttaaaggt ccaaatatca
    14101 tagtataaaa attctttaaa aaattccaaa ttttatatga ttttttgaaa attggaagaa
    14161 tgttagtttt tgccattttt ttggaagttt ttctccggat tttatggtac ttatatttca
    14221 aatataaatt tttgtaggaa ttcgagtatg cgagagagct atagtttact aaaaaaaagt
    14281 ggcaaaaact cagtaattgc cacttttttc aaaaatgttt tgaaaaattt tactatgata
    14341 tttggccatt ttggcaccat atgtacgtta ctttttacga aacactctaa tttttgaaat
    14401 ttgagtttca tgtttaatct tttgtataaa atttttatgg tccttttgtg acaggataaa
    14461 aaattgcaaa aaaatagaaa actttgtttc gtgaaaacac ctagtttaaa aattgtgggc
    14521 cacttagcga ttctagtcaa aaatcagaca aatgtcagct gccgtaactt tttccggatc
    14581 agggttatca aattttaatt ttaattgtaa atgtagaaaa atgatcggca acattttgct
    14641 aattgaaaat attttgaaaa ttgctatagt acatattaat ctagtattaa tatagtatta
    14701 atgttataat atcagttatg tgtttgaata actcaagcgt aagtttccgt aaaatatctt
    14761 taaattttgg taatttttta cggtttggta taaatttttg cattttttga ttttctacac
    14821 tttttttcga tttcgcattt tccgaatttc tctgaaataa atcgaacaat cggaaaattt
    14881 tcgagggcac ccttagccat gcagctcagg aatttgactt tatgttccca ttcaaatttt
    14941 tttaaaatta aataaaatgc taattgtaag ataaattaca aaaattatta aatctcatca
    15001 tccttatgat gatagtgcag tgcatgaata tatttttcca aataggatat gattcgtcga
    15061 gtagcttcac gtaatccgtt ttcttcaatc accatttcga actgcaattt tttttttgga
    15121 tttttgtaac aatattgtca taaattgtga ttttgatatt ttgcgtagag catcagaacc
    15181 tttctgcttt gaattttaat gggattaaaa attataaaaa acttgcaccc taattaaagc
    15241 gccaacttta ttatacatag ttgataattc gatggtatca gctcactcaa aggcggtaat
    15301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    15361 aaagaccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    15421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    15481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    15541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    15601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    15661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    15721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    15781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    15841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    15901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    15961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    16021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    16081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    16141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    16201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    16261 gggcttacca tctgacccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    16321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    16381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    16441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    16501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    16561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    16621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    16681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    16741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    16801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    16861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    16921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    16981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    17041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    17101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    17161 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    17221 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    17281 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    17341 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgcac tgagagtgca
    17401 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    17461 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    17521 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggat
    17581 ccgccaagct atcaactttg tatagaaaag ttg
//
