LOCUS       sequence-343550-       14923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi5605 site on chromosome II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14923)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 14923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD80
FEATURES             Location/Qualifiers
     source          1..14923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1338..1371)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1371..1382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2804..3127)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5045..5069
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5072..6097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6475..6508)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6877..6894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6878..6894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10202..10267
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             11123..11254
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             11255..11281
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      11290..11613
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     12903..12925
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12963..12981)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13049..13153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13154..13222,13223..14014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13372..13391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14185..14773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14674..14693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat gcaaatgttg
       61 agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt tctggaacaa
      121 ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa atgttaccac
      181 agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat tattattgta
      241 tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc aacgttttta
      301 agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt tattagataa
      361 tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt tttaaacgtt
      421 caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg caagcttctc
      481 cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt cgcaaaaata
      541 cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat ctacggttat
      601 acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa atttcagaac
      661 tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa aacatcgtag
      721 tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca gtacatttac
      781 agtattacct ctatattccg ttattggtag ttatgctttc cttattctat attcaattcc
      841 ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag gacgagagat
      901 tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc
      961 gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa agtgagagat
     1021 tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc
     1081 cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt ttgcaaaaac
     1141 tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata tcaacaagag
     1201 attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg agacccttgt
     1261 taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat tccatgatgg
     1321 tagcaaactc acttcgtata acttcgtata gtatacctta tacgaagtta tctgccctcg
     1381 attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt
     1441 tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac
     1501 ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc
     1561 acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga
     1621 tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac
     1681 taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga
     1741 gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt
     1801 atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga
     1861 taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta
     1921 attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc
     1981 aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga
     2041 tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag
     2101 actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc
     2161 ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca
     2221 ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc
     2281 ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga
     2341 ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca
     2401 ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca
     2461 ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca
     2521 agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga
     2581 gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac
     2641 ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag
     2701 tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc
     2761 taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg
     2821 cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc
     2881 gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat
     2941 tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac
     3001 ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga
     3061 gagaattttt tgacaaaaag aaagaatagt gatagagaag aagggaatgc ttgaaaggat
     3121 tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc
     3181 caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat
     3241 ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg
     3301 tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg
     3361 ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt
     3421 aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc
     3481 gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga
     3541 aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt
     3601 cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact
     3661 aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat
     3721 tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg
     3781 gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat
     3841 ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc
     3901 cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc
     3961 agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa
     4021 tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca
     4081 cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt
     4141 ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac
     4201 ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt
     4261 tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
     4321 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
     4381 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
     4441 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
     4501 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
     4561 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca
     4621 ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt
     4681 cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg
     4741 actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa
     4801 tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt
     4861 ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa
     4921 ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt
     4981 tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg
     5041 tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc
     5101 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5161 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5221 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5281 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5341 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5401 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5461 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5521 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5581 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5641 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5701 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5761 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5821 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5881 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5941 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6001 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6061 ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg
     6121 ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     6181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     6241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     6301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     6361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     6421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact
     6481 tcgtatagta taccttatac gaagttatac tagtatatat ttttatgttt tcttttaaac
     6541 cttcctccat atttctgaat tgaatgttgt tgcatattgt gtgtccttat ttcatttcca
     6601 tcatcctttt gtgtgtttcc cccttccacc aattcccccc acgtcctaat gaaatgaaag
     6661 cgagaagata aataaacttg cacattgtgt tcagatgaga ggagcggaat acgaattggg
     6721 agacggaaag agaaaaaaaa atgtttagat ttggattgaa ttaatttttt agaaagcaag
     6781 aaaaaaaaca caaagagaat aaataaataa taggaaaaaa aacaaatatt cagtatttaa
     6841 aatttgaaaa ttcaacgacg ttggcgtcga tcatcctgta aaacgacggc cagtgcatga
     6901 actcgacttt aatattcttt tctggtttca ccagaaaaat gctggactga aaaattgaat
     6961 ttcccgcgat ttgggaaaaa ccgcagttta agcttttggc atgaatacag tgaacacagg
     7021 aacaaaaaat tgacttgtta ttttgagttt atattaaatg ttaattttgc atctcatatt
     7081 ttcacaaaac ttgtttgctt gaaaaatgtg caaataacaa gtgaacatac atcaaggttt
     7141 atgcagatca gcactgatgc aactgatagc aaaactgata agggtcaaaa tttcagtggc
     7201 cagcacaaac acatacacat tcgtagttcg ttttgtgttt cgtgtattta gcatttattt
     7261 ttcaattccg gcaatttgcc gatttgccgg aaattttgat ttttggcaaa ttgccagttt
     7321 gccgtttgcc gaacatcaat ttgccgaaat tttttagagg gatctttata agacggaaac
     7381 acttaaaatt gtgctttttt ggaatttttt tcaatgtttc aatagaatta gcttacattt
     7441 aaaattagat gtaggaacgt ttataggacg cttacaattt tgccgattaa aatcgaagtt
     7501 ctgaactttt caaaaaatgt gcaaaatttt ttttgacaaa tttgacaaat cggccggttt
     7561 gtcgattgtc cggaattttt cagtttcggc gatttaccgg tttgccggaa aacatcgttt
     7621 gccacccaca cacacataaa gagacgaaac aacacaccat cttcaaaaaa tttgtgaatc
     7681 ttcatttcca cgtggtttgc agggtttccc atccacccca ctctatctct ccaagttgaa
     7741 ttttggcaaa attgaccaca caccgatcaa aaactattgt gtgtgtgtgt atttgtgtct
     7801 tcaataattc ctgtctgtgt ctcgcactat ctattgacta caagaacgtg atcgcagctc
     7861 tacgaaacct ttactttatt ttagaaatat tttttcatgc tattagcaaa aaaaaagttt
     7921 ttttttgtct aagtacactt aaaattttcc aaaattttta agatttttca aaatgtgcga
     7981 tcaaagatct gacaaaatgc caaaattttg aagaggaaat ttagtcggct tccaaaatta
     8041 taagtggcag aaattgagca attgtcactt tttgacagta aatagaaaat tttttgaaaa
     8101 attttgaaac gctttatttt gttacttggt cattttggca ccataggagt agtttttaac
     8161 attttcccca ctggcgctac ttcattttaa acttttccaa agattttaat ttttaatttt
     8221 aaaaaatttc atataattcg tctgcgtctt acagtttcag gcttcaaaaa tctattacat
     8281 atcttatctt tgaattcaga tgcaaaagag aatcgccttg tcgtttccag aagaagtact
     8341 cgagcacgtc ttctccttca tccaactcga caaggaccgt aactccgtct ccctcgtctg
     8401 caagtcctgg tacgagatcg agcgttggtg ccgtcgtaag gtcttcatcg gaaactgcta
     8461 cgccgtctcc ccagccaccg tcatccgtcg tttcccaaag gtccgttccg tcgagctcaa
     8521 gggaaagcca cacttcgccg acttcaacct cgtcccagac ggatggggag gatacgtcta
     8581 cccatggatc gaggccatgt cctcctccta cacctggctc gaggagatcc gtctcaagcg
     8641 tatggtcgtc accgacgact gcctcgagct catcgccaag tccttcaaga acttcaaggt
     8701 cctcgtcctc tcctcctgcg agggattctc caccgacgga ctcgccgcca tcgccgccac
     8761 ctgccgtaac ctcaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa
     8821 ttttcaggag ctcgacctcc gtgagtccga cgtcgacgag gtctccggac actggctctc
     8881 ccacttccca gacacctaca cctccctcgt ctccctcaac atctcctgcc tcgcctccga
     8941 ggtctccttc tccgccctcg agcgtctcgt cacccgttgc ccaaacctca agtccctcaa
     9001 gctcaaccgt gccgtcccac tcgagaagct cgccaccctc ctccaacgtg ccccacaact
     9061 cgaggagctc ggaaccggag gatacaccgc cgaggtccgt ccagacgtct actccggact
     9121 ctccgtcgcc ctctccggat gcaaggtaag tttaaacagt tcggtactaa ctaaccatac
     9181 atatttaaat tttcaggagc tccgttgcct ctccggattc tgggacgccg tcccagccta
     9241 cctcccagcc gtctactccg tctgctcccg tctcaccacc ctcaacctct cctacgccac
     9301 cgtccaatcc tacgacctcg tcaagctcct ctgccaatgc ccaaagctcc aacgtctctg
     9361 ggtcctcgac tacatcgagg acgccggact cgaggtcctc gcctccacct gcaaggacct
     9421 ccgtgagctc cgtgtcttcc catccgagcc attcgtcatg gagccaaacg tcgccctcac
     9481 cgagcaagga ctcgtctccg tctccatggg atgcccaaag ctcgagtccg tcctctactt
     9541 ctgccgtcaa atgaccaacg ccgccctcat caccatcgcc cgtaaccgtc caaacatgac
     9601 ccgtttccgt ctctgcatca tcgagccaaa ggccccagac tacctcaccc tcgagccact
     9661 cgacatcgga ttcggagcca tcgtcgagca ctgcaaggac ctccgtcgtc tctccctctc
     9721 cggactcctc accgacaagg tcttcgagta catcggaacc tacgccaaga agatggagat
     9781 gctctccgtc gccttcgccg gagactccga cctcggactc caccacgtcc tctccggatg
     9841 cgactccctc cgtaagctcg agatccgtga ctgcccattc ggagacaagg ccctcctcgc
     9901 caacgcctcc aagctcgaga ccatgcgttc cctctggatg tcctcctgct ccgtctcctt
     9961 cggagcctgc aagctcctcg gacaaaagat gccaaagctc aacgtcgagg tcatcgacga
    10021 gcgtggagcc ccagactccc gtccagagtc ctgcccagtc gagcgtgtct tcatctaccg
    10081 taccgtcgcc ggaccacgtt tcgacatgcc aggattcgtc tggaacatgg accaagactc
    10141 caccatgcgt ttctcccgtc aaatcatcac caccaacgga ctcggaggag gaggatcccc
    10201 agtcaagcaa accctcaact tcgacctcct caagctcgcc ggagacgtcg agtccaaccc
    10261 aggaccaatg gtttcggaat tgattaagga aaatatgcac atgaagctct acatggaggg
    10321 aaccgtcgac aaccaccact tcaagtgcac ctccgaggga gagggaaagc catacgaggg
    10381 aacccaaacc atgcgtatca aggtcgtcga gggaggacca ctcccattcg ccttcgacat
    10441 cctcgccacc tccttcctct acggatccaa ggtaagttta aacatatata tactaactaa
    10501 ccctgattat ttaaattttc agaccttcat caaccacacc caaggaatcc cagacttctt
    10561 caagcaatcc ttcccagagg gattcacctg ggagcgtgtc accacctacg aggacggagg
    10621 agtcctcacc gccacccaag acacctccct ccaagacgga tgcctcatct acaacgtcaa
    10681 ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agatccgtgg
    10741 agtcaacttc acctccaacg gaccagtcat gcaaaagaag accctcggat gggaggcctt
    10801 caccgagacc ctctacccag ccgacggagg actcgaggga cgtaacgaca tggccctcaa
    10861 gctcgtcgga ggatcccacc tcatcgccaa cgccaaggta agtttaaaca tgattttact
    10921 aactaactaa tctgatttaa attttcagac cacctaccgt tccaagaagc cagccaagaa
    10981 cctcaagatg ccaggagtct actacgtcga ctaccgtctc gagcgtatca aggaggccaa
    11041 caacgagacc tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg acctcccatc
    11101 caagctcgga cacaagctca accctaaaga tccagccaaa cctccggcca aggcacaagt
    11161 tgtgggatgg ccaccggtga gatcataccg gaagaacgtg atggtttcct gccaaaaatc
    11221 aagcggtggc ccggaggcgg cggcgttcgt gaaaccagcc gccaagcgtg tcaagctcga
    11281 ctaagataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt
    11341 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt
    11401 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt
    11461 ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt
    11521 tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag
    11581 aggaaacttt attgtgccgc caagaaaaaa gtctcacgta cggtgcgcgc gatgccgtat
    11641 cacaactgag aaaaaagaca aaacttattg aacagtgttc ctttcaaaaa attattttgt
    11701 aaaaaatcga gtttttctcc aaaataacgc acgcaatcaa aaacaagaaa actaacgtgt
    11761 tctcctgggc gtggaactcc ttatcaagat taccattatt gtctgtcaga attgatattt
    11821 caattatttc atatctagac aaacccgggt gtcttgcatt tattagtact gtgagtttac
    11881 atacaaacga ggcttactca gataacatag agtcacctct aattagcaaa aacccatttc
    11941 taattagcta tttcgaccag ttctaaagtc accgtaatta gaaatggttt ttgtagcttt
    12001 tctaattaga aatgaccccg agataagtga aatatggcat tttctgggtt ttctagtaag
    12061 aaatgatccc gcacgtagtg attccgcgtt acaagccaaa ttcaaattaa aaaagtatat
    12121 tacacaattc aagttacatt attttcagga aacaatggcg aagagcaaag cagagattca
    12181 aaaggaggaa agagacaaca gatatgaacg aagacttctt gaaaagagaa agagacatga
    12241 ggtgagtaac catgataata aataatcaag taagggctat agatttataa attctggagt
    12301 taacagtttc gaaaaaaagt agttttcata tgtgcgaagg aatatcaaaa ataggggaat
    12361 agcctaaacg agtgagaaaa ttaaatgttt gttatttttt caaaaatgtc tgcagggccg
    12421 ttatcctttc aaatgcactc tatcaaacat agatcatatc catcatatct tatccagaat
    12481 ttatatcata tccaagattg agagcgtttc aaggaaaatc tcaaacaacc atacaatttt
    12541 gtagatcgac aacaaggcct ccacttcttc cacgtgacgt cgtgactggg aaaaccctgg
    12601 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    12661 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
    12721 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
    12781 cagtgcaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
    12841 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    12901 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
    12961 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
    13021 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    13081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    13141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    13201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    13261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    13321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    13381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    13441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    13501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    13561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    13621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    13681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    13741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    13801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    13861 tgagcgtggg tctcgcggta tcattgcagc actggggtca gatggtaagc cctcccgtat
    13921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    13981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    14041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    14101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    14161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    14221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    14281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    14341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    14401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    14461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    14521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    14581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    14641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    14701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    14761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggtct tttgctggcc
    14821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    14881 ctttgagtga gctgatacca gcggataaca atttcacagg gcc
//
