LOCUS       sequence-343552-       14670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi5605 site on chromosome II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14670)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 14670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD81
FEATURES             Location/Qualifiers
     source          1..14670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1338..1371)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1371..1382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2804..3127)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5045..5069
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5072..6097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6475..6508)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6877..6894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6878..6894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9949..10014
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             10870..11001
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             11002..11028
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      11037..11360
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     12650..12672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12710..12728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12796..12900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12901..12969,12970..13761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13119..13138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13932..14520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14421..14440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat gcaaatgttg
       61 agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt tctggaacaa
      121 ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa atgttaccac
      181 agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat tattattgta
      241 tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc aacgttttta
      301 agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt tattagataa
      361 tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt tttaaacgtt
      421 caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg caagcttctc
      481 cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt cgcaaaaata
      541 cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat ctacggttat
      601 acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa atttcagaac
      661 tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa aacatcgtag
      721 tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca gtacatttac
      781 agtattacct ctatattccg ttattggtag ttatgctttc cttattctat attcaattcc
      841 ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag gacgagagat
      901 tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc
      961 gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa agtgagagat
     1021 tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc
     1081 cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt ttgcaaaaac
     1141 tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata tcaacaagag
     1201 attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg agacccttgt
     1261 taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat tccatgatgg
     1321 tagcaaactc acttcgtata acttcgtata gtatacctta tacgaagtta tctgccctcg
     1381 attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt
     1441 tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac
     1501 ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc
     1561 acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga
     1621 tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac
     1681 taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga
     1741 gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt
     1801 atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga
     1861 taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta
     1921 attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc
     1981 aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga
     2041 tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag
     2101 actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc
     2161 ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca
     2221 ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc
     2281 ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga
     2341 ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca
     2401 ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca
     2461 ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca
     2521 agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga
     2581 gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac
     2641 ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag
     2701 tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc
     2761 taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg
     2821 cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc
     2881 gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat
     2941 tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac
     3001 ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga
     3061 gagaattttt tgacaaaaag aaagaatagt gatagagaag aagggaatgc ttgaaaggat
     3121 tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc
     3181 caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat
     3241 ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg
     3301 tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg
     3361 ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt
     3421 aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc
     3481 gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga
     3541 aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt
     3601 cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact
     3661 aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat
     3721 tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg
     3781 gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat
     3841 ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc
     3901 cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc
     3961 agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa
     4021 tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca
     4081 cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt
     4141 ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac
     4201 ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt
     4261 tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
     4321 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
     4381 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
     4441 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
     4501 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
     4561 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca
     4621 ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt
     4681 cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg
     4741 actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa
     4801 tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt
     4861 ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa
     4921 ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt
     4981 tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg
     5041 tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc
     5101 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5161 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5221 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5281 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5341 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5401 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5461 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5521 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5581 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5641 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5701 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5761 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5821 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5881 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5941 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6001 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6061 ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg
     6121 ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     6181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     6241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     6301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     6361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     6421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact
     6481 tcgtatagta taccttatac gaagttatac tagtatatat ttttatgttt tcttttaaac
     6541 cttcctccat atttctgaat tgaatgttgt tgcatattgt gtgtccttat ttcatttcca
     6601 tcatcctttt gtgtgtttcc cccttccacc aattcccccc acgtcctaat gaaatgaaag
     6661 cgagaagata aataaacttg cacattgtgt tcagatgaga ggagcggaat acgaattggg
     6721 agacggaaag agaaaaaaaa atgtttagat ttggattgaa ttaatttttt agaaagcaag
     6781 aaaaaaaaca caaagagaat aaataaataa taggaaaaaa aacaaatatt cagtatttaa
     6841 aatttgaaaa ttcaacgacg ttggcgtcga tcatcctgta aaacgacggc cagtgcatgg
     6901 atcttcatcc cttcaacatt tggcagaaaa gtgcaattcg tcttttacag gaattcgatt
     6961 tccgatctct tgcacaactc gaggcgtcac tccagatgga ttaggaactg tttccctttg
     7021 tagtacatgt tgggtgtggc gacgtcttcc gagtacctac tatcctgcat atcttaacga
     7081 agtcgtgtgc gattatgcgg acacatcgtg tctgagtggt agtgaaaaga cagagaaagt
     7141 gggggaaaaa gaaaatacca ttttttcagg atacgcgtca tgccaaactg gaacccaaca
     7201 gttgaatgtg ctacgaaatg acagcgggaa attgattcca atatcggtat cagctggaat
     7261 caattgtgaa tgtagattag cagttggatc aacacttgaa tcactcgtac ttggacaagg
     7321 aatttccaaa gcaatgccgc caattgacac aactagtaca aaaccaccaa atttggcaac
     7381 aagcacaaca agccattcct aatttatttg ggtattttac tcttgataat tgtattaatg
     7441 atcatttaat gttgttgaat atcacacatt taacatattc aatcgaagaa atataacgac
     7501 gttccacaac atccagtata cgtcttcctc tgtattgagt tttattcttt gacattgtgt
     7561 catgtttcag aacatagaaa tataccaaag tgttgagaag cattgtataa ttcaattgaa
     7621 tctttttcag tttacctctt ctcctttttc accgtttccg tcatcaaaac gccgttattc
     7681 aaggtgagat tatggcgata aatgaatgcc attcacaaaa ttgcaagaca ctattaaaga
     7741 agaagaggtt tagagatttg taccgatcat agcttcttct gagaaagcga ccttggcggc
     7801 cgtacttgaa gagaagcaat ttttatcgcc gtagaagatg ttgcctgcca cccaccctgt
     7861 acgttgtttt tttgcttatc aacatctgaa ttttgttgtc ttttgcaccg cctactttta
     7921 ctatggaatt tgaggaaata taatatgaat tgaagagaat acgctacctg tcaaagaaac
     7981 gtcacggtta gggtagtata aaagggaagg gtaacactga ataaggtacc tggcttactt
     8041 tcagtcatgc aaaagagaat cgccttgtcg tttccagaag aagtactcga gcacgtcttc
     8101 tccttcatcc aactcgacaa ggaccgtaac tccgtctccc tcgtctgcaa gtcctggtac
     8161 gagatcgagc gttggtgccg tcgtaaggtc ttcatcggaa actgctacgc cgtctcccca
     8221 gccaccgtca tccgtcgttt cccaaaggtc cgttccgtcg agctcaaggg aaagccacac
     8281 ttcgccgact tcaacctcgt cccagacgga tggggaggat acgtctaccc atggatcgag
     8341 gccatgtcct cctcctacac ctggctcgag gagatccgtc tcaagcgtat ggtcgtcacc
     8401 gacgactgcc tcgagctcat cgccaagtcc ttcaagaact tcaaggtcct cgtcctctcc
     8461 tcctgcgagg gattctccac cgacggactc gccgccatcg ccgccacctg ccgtaacctc
     8521 aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcaggagctc
     8581 gacctccgtg agtccgacgt cgacgaggtc tccggacact ggctctccca cttcccagac
     8641 acctacacct ccctcgtctc cctcaacatc tcctgcctcg cctccgaggt ctccttctcc
     8701 gccctcgagc gtctcgtcac ccgttgccca aacctcaagt ccctcaagct caaccgtgcc
     8761 gtcccactcg agaagctcgc caccctcctc caacgtgccc cacaactcga ggagctcgga
     8821 accggaggat acaccgccga ggtccgtcca gacgtctact ccggactctc cgtcgccctc
     8881 tccggatgca aggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
     8941 caggagctcc gttgcctctc cggattctgg gacgccgtcc cagcctacct cccagccgtc
     9001 tactccgtct gctcccgtct caccaccctc aacctctcct acgccaccgt ccaatcctac
     9061 gacctcgtca agctcctctg ccaatgccca aagctccaac gtctctgggt cctcgactac
     9121 atcgaggacg ccggactcga ggtcctcgcc tccacctgca aggacctccg tgagctccgt
     9181 gtcttcccat ccgagccatt cgtcatggag ccaaacgtcg ccctcaccga gcaaggactc
     9241 gtctccgtct ccatgggatg cccaaagctc gagtccgtcc tctacttctg ccgtcaaatg
     9301 accaacgccg ccctcatcac catcgcccgt aaccgtccaa acatgacccg tttccgtctc
     9361 tgcatcatcg agccaaaggc cccagactac ctcaccctcg agccactcga catcggattc
     9421 ggagccatcg tcgagcactg caaggacctc cgtcgtctct ccctctccgg actcctcacc
     9481 gacaaggtct tcgagtacat cggaacctac gccaagaaga tggagatgct ctccgtcgcc
     9541 ttcgccggag actccgacct cggactccac cacgtcctct ccggatgcga ctccctccgt
     9601 aagctcgaga tccgtgactg cccattcgga gacaaggccc tcctcgccaa cgcctccaag
     9661 ctcgagacca tgcgttccct ctggatgtcc tcctgctccg tctccttcgg agcctgcaag
     9721 ctcctcggac aaaagatgcc aaagctcaac gtcgaggtca tcgacgagcg tggagcccca
     9781 gactcccgtc cagagtcctg cccagtcgag cgtgtcttca tctaccgtac cgtcgccgga
     9841 ccacgtttcg acatgccagg attcgtctgg aacatggacc aagactccac catgcgtttc
     9901 tcccgtcaaa tcatcaccac caacggactc ggaggaggag gatccccagt caagcaaacc
     9961 ctcaacttcg acctcctcaa gctcgccgga gacgtcgagt ccaacccagg accaatggtt
    10021 tcggaattga ttaaggaaaa tatgcacatg aagctctaca tggagggaac cgtcgacaac
    10081 caccacttca agtgcacctc cgagggagag ggaaagccat acgagggaac ccaaaccatg
    10141 cgtatcaagg tcgtcgaggg aggaccactc ccattcgcct tcgacatcct cgccacctcc
    10201 ttcctctacg gatccaaggt aagtttaaac atatatatac taactaaccc tgattattta
    10261 aattttcaga ccttcatcaa ccacacccaa ggaatcccag acttcttcaa gcaatccttc
    10321 ccagagggat tcacctggga gcgtgtcacc acctacgagg acggaggagt cctcaccgcc
    10381 acccaagaca cctccctcca agacggatgc ctcatctaca acgtcaaggt aagtttaaac
    10441 agttcggtac taactaacca tacatattta aattttcaga tccgtggagt caacttcacc
    10501 tccaacggac cagtcatgca aaagaagacc ctcggatggg aggccttcac cgagaccctc
    10561 tacccagccg acggaggact cgagggacgt aacgacatgg ccctcaagct cgtcggagga
    10621 tcccacctca tcgccaacgc caaggtaagt ttaaacatga ttttactaac taactaatct
    10681 gatttaaatt ttcagaccac ctaccgttcc aagaagccag ccaagaacct caagatgcca
    10741 ggagtctact acgtcgacta ccgtctcgag cgtatcaagg aggccaacaa cgagacctac
    10801 gtcgagcaac acgaggtcgc cgtcgcccgt tactgcgacc tcccatccaa gctcggacac
    10861 aagctcaacc ctaaagatcc agccaaacct ccggccaagg cacaagttgt gggatggcca
    10921 ccggtgagat cataccggaa gaacgtgatg gtttcctgcc aaaaatcaag cggtggcccg
    10981 gaggcggcgg cgttcgtgaa accagccgcc aagcgtgtca agctcgacta agataaatgc
    11041 aaaatccttt caagcattcc cttcttctct atcactcttc tttctttttg tcaaaaaatt
    11101 ctctcgctaa tttatttgct tttttaatgt tattatttta tgacttttta tagtcactga
    11161 aaagtttgca tctgagtgaa gtgaatgcta tcaaaatgtg attctgtctg atgtactttc
    11221 acaatctctc ttcaattcca ttttgaagtg ctttaaaccc gaaaggttga gaaaaatgcg
    11281 agcgctcaaa tatttgtatt gtgttcgttg agtgacccaa caaaaagagg aaactttatt
    11341 gtgccgccaa gaaaaaagtc tcacgtacgg tgcgcgcgat gccgtatcac aactgagaaa
    11401 aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt attttgtaaa aaatcgagtt
    11461 tttctccaaa ataacgcacg caatcaaaaa caagaaaact aacgtgttct cctgggcgtg
    11521 gaactcctta tcaagattac cattattgtc tgtcagaatt gatatttcaa ttatttcata
    11581 tctagacaaa cccgggtgtc ttgcatttat tagtactgtg agtttacata caaacgaggc
    11641 ttactcagat aacatagagt cacctctaat tagcaaaaac ccatttctaa ttagctattt
    11701 cgaccagttc taaagtcacc gtaattagaa atggtttttg tagcttttct aattagaaat
    11761 gaccccgaga taagtgaaat atggcatttt ctgggttttc tagtaagaaa tgatcccgca
    11821 cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa agtatattac acaattcaag
    11881 ttacattatt ttcaggaaac aatggcgaag agcaaagcag agattcaaaa ggaggaaaga
    11941 gacaacagat atgaacgaag acttcttgaa aagagaaaga gacatgaggt gagtaaccat
    12001 gataataaat aatcaagtaa gggctataga tttataaatt ctggagttaa cagtttcgaa
    12061 aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata ggggaatagc ctaaacgagt
    12121 gagaaaatta aatgtttgtt attttttcaa aaatgtctgc agggccgtta tcctttcaaa
    12181 tgcactctat caaacataga tcatatccat catatcttat ccagaattta tatcatatcc
    12241 aagattgaga gcgtttcaag gaaaatctca aacaaccata caattttgta gatcgacaac
    12301 aaggcctcca cttcttccac gtgacgtcgt gactgggaaa accctggcgt tacccaactt
    12361 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    12421 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
    12481 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tgcaatctgc
    12541 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
    12601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    12661 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
    12721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
    12781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    12841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    12901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    12961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    13021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    13081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    13141 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    13201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    13261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    13321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    13381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    13441 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    13501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    13561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    13621 cgcggtatca ttgcagcact ggggtcagat ggtaagccct cccgtatcgt agttatctac
    13681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    13741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    13801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    13861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    13921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    13981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    14041 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    14101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    14161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    14221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    14281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    14341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    14401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    14461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    14521 aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt tgctcacatg
    14581 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    14641 gataccagcg gataacaatt tcacagggcc
//
