LOCUS       sequence-343553-       13896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms
            for insertion in ttTi5605 site on chromosome II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 13896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD82
FEATURES             Location/Qualifiers
     source          1..13896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     terminator      complement(3203..3526)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(3535..3561)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(3562..3693)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             complement(4549..4614)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(6895..6912)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6895..6911)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7281..7314
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    7667..7691
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7692..8717)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7692..8717)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8720..8744)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      10662..10985
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             12407..12418
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    12418..12451
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctgacc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg cactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtc acgtggaaga agtggaggcc ttgttgtcga tctacaaaat
     2281 tgtatggttg tttgagattt tccttgaaac gctctcaatc ttggatatga tataaattct
     2341 ggataagata tgatggatat gatctatgtt tgatagagtg catttgaaag gataacggcc
     2401 ctgcagacat ttttgaaaaa ataacaaaca tttaattttc tcactcgttt aggctattcc
     2461 cctatttttg atattccttc gcacatatga aaactacttt ttttcgaaac tgttaactcc
     2521 agaatttata aatctatagc ccttacttga ttatttatta tcatggttac tcacctcatg
     2581 tctctttctc ttttcaagaa gtcttcgttc atatctgttg tctctttcct ccttttgaat
     2641 ctctgctttg ctcttcgcca ttgtttcctg aaaataatgt aacttgaatt gtgtaatata
     2701 cttttttaat ttgaatttgg cttgtaacgc ggaatcacta cgtgcgggat catttcttac
     2761 tagaaaaccc agaaaatgcc atatttcact tatctcgggg tcatttctaa ttagaaaagc
     2821 tacaaaaacc atttctaatt acggtgactt tagaactggt cgaaatagct aattagaaat
     2881 gggtttttgc taattagagg tgactctatg ttatctgagt aagcctcgtt tgtatgtaaa
     2941 ctcacagtac taataaatgc aagacacccg ggtttgtcta gatatgaaat aattgaaata
     3001 tcaattctga cagacaataa tggtaatctt gataaggagt tccacgccca ggagaacacg
     3061 ttagttttct tgtttttgat tgcgtgcgtt attttggaga aaaactcgat tttttacaaa
     3121 ataatttttt gaaaggaaca ctgttcaata agttttgtct tttttctcag ttgtgatacg
     3181 gcatcgcgcg caccgtacgt gagacttttt tcttggcggc acaataaagt ttcctctttt
     3241 tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt
     3301 tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga
     3361 atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt
     3421 cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga
     3481 aagaagagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat cttagtcgag
     3541 cttgacacgc ttggcggctg gtttcacgaa cgccgccgcc tccgggccac cgcttgattt
     3601 ttggcaggaa accatcacgt tcttccggta tgatctcacc ggtggccatc ccacaacttg
     3661 tgccttggcc ggaggtttgg ctggatcttt agggttgagc ttgtgtccga gcttggatgg
     3721 gaggtcgcag taacgggcga cggcgacctc gtgttgctcg acgtaggtct cgttgttggc
     3781 ctccttgata cgctcgagac ggtagtcgac gtagtagact cctggcatct tgaggttctt
     3841 ggctggcttc ttggaacggt aggtggtctg aaaatttaaa tcagattagt tagttagtaa
     3901 aatcatgttt aaacttacct tggcgttggc gatgaggtgg gatcctccga cgagcttgag
     3961 ggccatgtcg ttacgtccct cgagtcctcc gtcggctggg tagagggtct cggtgaaggc
     4021 ctcccatccg agggtcttct tttgcatgac tggtccgttg gaggtgaagt tgactccacg
     4081 gatctgaaaa tttaaatatg tatggttagt tagtaccgaa ctgtttaaac ttaccttgac
     4141 gttgtagatg aggcatccgt cttggaggga ggtgtcttgg gtggcggtga ggactcctcc
     4201 gtcctcgtag gtggtgacac gctcccaggt gaatccctct gggaaggatt gcttgaagaa
     4261 gtctgggatt ccttgggtgt ggttgatgaa ggtctgaaaa tttaaataat cagggttagt
     4321 tagtatatat atgtttaaac ttaccttgga tccgtagagg aaggaggtgg cgaggatgtc
     4381 gaaggcgaat gggagtggtc ctccctcgac gaccttgata cgcatggttt gggttccctc
     4441 gtatggcttt ccctctccct cggaggtgca cttgaagtgg tggttgtcga cggttccctc
     4501 catgtagagc ttcatgtgca tattttcctt aatcaattcc gaaaccattg gtcctgggtt
     4561 ggactcgacg tctccggcga gcttgaggag gtcgaagttg agggtttgct tgactgggga
     4621 tcctcctcct ccgagtccgt tggtggtgat gatttgacgg gagaaacgca tggtggagtc
     4681 ttggtccatg ttccagacga atcctggcat gtcgaaacgt ggtccggcga cggtacggta
     4741 gatgaagaca cgctcgactg ggcaggactc tggacgggag tctggggctc cacgctcgtc
     4801 gatgacctcg acgttgagct ttggcatctt ttgtccgagg agcttgcagg ctccgaagga
     4861 gacggagcag gaggacatcc agagggaacg catggtctcg agcttggagg cgttggcgag
     4921 gagggccttg tctccgaatg ggcagtcacg gatctcgagc ttacggaggg agtcgcatcc
     4981 ggagaggacg tggtggagtc cgaggtcgga gtctccggcg aaggcgacgg agagcatctc
     5041 catcttcttg gcgtaggttc cgatgtactc gaagaccttg tcggtgagga gtccggagag
     5101 ggagagacga cggaggtcct tgcagtgctc gacgatggct ccgaatccga tgtcgagtgg
     5161 ctcgagggtg aggtagtctg gggcctttgg ctcgatgatg cagagacgga aacgggtcat
     5221 gtttggacgg ttacgggcga tggtgatgag ggcggcgttg gtcatttgac ggcagaagta
     5281 gaggacggac tcgagctttg ggcatcccat ggagacggag acgagtcctt gctcggtgag
     5341 ggcgacgttt ggctccatga cgaatggctc ggatgggaag acacggagct cacggaggtc
     5401 cttgcaggtg gaggcgagga cctcgagtcc ggcgtcctcg atgtagtcga ggacccagag
     5461 acgttggagc tttgggcatt ggcagaggag cttgacgagg tcgtaggatt ggacggtggc
     5521 gtaggagagg ttgagggtgg tgagacggga gcagacggag tagacggctg ggaggtaggc
     5581 tgggacggcg tcccagaatc cggagaggca acggagctcc tgaaaattta aatatgtatg
     5641 gttagttagt accgaactgt ttaaacttac cttgcatccg gagagggcga cggagagtcc
     5701 ggagtagacg tctggacgga cctcggcggt gtatcctccg gttccgagct cctcgagttg
     5761 tggggcacgt tggaggaggg tggcgagctt ctcgagtggg acggcacggt tgagcttgag
     5821 ggacttgagg tttgggcaac gggtgacgag acgctcgagg gcggagaagg agacctcgga
     5881 ggcgaggcag gagatgttga gggagacgag ggaggtgtag gtgtctggga agtgggagag
     5941 ccagtgtccg gagacctcgt cgacgtcgga ctcacggagg tcgagctcct gaaaatttaa
     6001 ataatcaggg ttagttagta tatatatgtt taaacttacc ttgaggttac ggcaggtggc
     6061 ggcgatggcg gcgagtccgt cggtggagaa tccctcgcag gaggagagga cgaggacctt
     6121 gaagttcttg aaggacttgg cgatgagctc gaggcagtcg tcggtgacga ccatacgctt
     6181 gagacggatc tcctcgagcc aggtgtagga ggaggacatg gcctcgatcc atgggtagac
     6241 gtatcctccc catccgtctg ggacgaggtt gaagtcggcg aagtgtggct ttcccttgag
     6301 ctcgacggaa cggacctttg ggaaacgacg gatgacggtg gctggggaga cggcgtagca
     6361 gtttccgatg aagaccttac gacggcacca acgctcgatc tcgtaccagg acttgcagac
     6421 gagggagacg gagttacggt ccttgtcgag ttggatgaag gagaagacgt gctcgagtac
     6481 ttcttctgga aacgacaagg cgattctctt ttgcatgatt tctcgcttct ttcaaatggt
     6541 ttctctacct caaaactaat gtggtgctcc cttagtcgag gttgatgatg cgagtgatga
     6601 gtgcctgctt cttcctcggc aggaggatgt tggcacccgc aagccacgcc cctctttggg
     6661 gattctacga ggtgtaaagc tgtgtgcggc agcggcagcg gcgggagaga cgggaacccg
     6721 gcttcccggg aatacacgaa gcgagagacg cctacgcgta gtggagggtg ccagagaaga
     6781 tagtggaagt ctcaagaatt ccattggagc acgaagaaaa aaccagggat agagatagat
     6841 cgggagttgg taagatcggg aaaaatgaat tcagagagta cggtcggtcc atgcactggc
     6901 cgtcgtttta caggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa
     6961 tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa
     7021 aaaattaatt caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat
     7081 tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat
     7141 taggacgtgg ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat
     7201 aaggacacac aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa
     7261 acataaaaat atatactagt ataacttcgt ataaggtata ctatacgaag ttattaaaaa
     7321 ttgcggtcat aaactgaaac gtaacatatg ataaggtatt ttgtgtgcgg ttttttctat
     7381 gatgtttcaa aaaaatttat cagaagtaaa aaacataaga agtgtacaca gaacattgtg
     7441 tagattttgg gatagaatta ttattaatgg ggggagggag cacaattttt ttcgactttt
     7501 tattacgaat taattctatt tttgaatcta cacaatttca ttgttagagg tgacttaaaa
     7561 gaagctaaaa aacaaagaaa ttaagagaag ttttttgata ataacaaaaa tagggggtgg
     7621 gagcacaggg agaaagagca tgtagggatg ttgaagagta attggaccca ctttgtacaa
     7681 gaaagctggg tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     7741 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     7801 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     7861 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     7921 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     7981 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     8041 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     8101 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     8161 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     8221 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     8281 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     8341 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     8401 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     8461 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     8521 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     8581 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     8641 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     8701 agttcaggct ttttcatgga gcctgctttt ttgtacaaac ttgcggcacc gcctgacata
     8761 ttaccttaaa attcaaaaat taatttcaga tcagtcataa ccaagaaaac aaaataaacg
     8821 ttaggacaga aaattcaaat taattaaaaa ttatgctgaa taagaaaatt acatgcaaag
     8881 ccttattttc ctcatagaaa cagttgaata acatattttt taattgaaaa catagcaaaa
     8941 ttcgtggtaa aatttgtcaa aaccttggaa aaaatcgaaa aaagaacatt tcccgatgca
     9001 ttttcctatt tctcgttgcc gacaacgtat aaacgttgaa tactcagtca acgaatttgg
     9061 ctgctggctt ggactctcgc acggaagaga cgcgtgtgta gatttacgac gaaagcaaaa
     9121 atgatcacca aaaacggaac gttgagctgg acggaaatag tggtaaagtg acatgattat
     9181 agtttgaaga tttctaattt cacaattaga gcaaatgttg ttcggtattt attttcaacg
     9241 gtatttatac tattttccac ctttttctag aacattcgag ctgcttgttg caaaaggagg
     9301 gcgactcaca ttcggtacat ggaaaagtag tgtacacaat aaagagaccc agatacattt
     9361 tccgtctgcg tctctttgca cccaccggga gtattttcaa acgaatgcat ctaggacctt
     9421 ctagaacatt ctgtaaggct gcagaatgcg ggtatataag gaaagcgggc tcagaggaag
     9481 ccaacacgct ttgttctagt gcatctaaaa aacttcgaaa atatgtcaaa tcttctcact
     9541 gttcatcaaa atcttccagc acttccagtt gacgcgacaa gtgatgaagt tcgcaagaac
     9601 ctcatggata tgttccgtga cagacaagcc ttctccgagc acacctggaa gatgcttctc
     9661 tcagtgtgcc gttcttgggc tgcctggtgc aagcttaaca accgcaagtg gttcccagcc
     9721 gagccagaag acgttcgtga ctacttgctc tacctccaag ctcgtggatt ggcggtgaag
     9781 acgatccaac aacatctcgg acagctcaat atgctccatc gccgatctgg acttccacgc
     9841 ccatcagatt ctaatgccgt atctctcgta atgagacgta tccgcaagga aaatgttgat
     9901 gctggagaga gagccaagca agccctcgcc ttcgaaaggt aagtttaaat attaatgtat
     9961 tcaatcctaa gacttgacga aattttcaga accgacttcg atcaggtccg ttctcttatg
    10021 gaaaattccg atcgttgcca ggacatccga aacctcgcct tcctcggaat tgcttacaac
    10081 acacttctcc gtatcgccga aattgcccgc attcgcgtca aggatattag tcgcacagac
    10141 ggaggaagaa tgctcatcca catcggacgt acaaaaacgc tggtctcgac tgccggagtc
    10201 gaaaaagcgc tctcccttgg agttaccaag ctcgttgagc gctggatttc ggtttccgga
    10261 gtcgccgatg atccaaacaa ctacctgttc tgtcgtgttc gcaagaacgg agttgctgcc
    10321 ccgagtgcaa catcacaact ctcgactcga gctcttgaag gtaagtttaa acaacatatt
    10381 agctaagaat aagctgaaaa taaattttca ggaatcttcg aagctaccca ccgtctcatc
    10441 tacggagcca aggatgattc cggacaacgt tacttggctt ggtctggaca ttccgcccgt
    10501 gtcggagctg cccgtgacat ggctcgcgct ggagtttcca tcccagagat tatgcaagcc
    10561 ggaggttgga ccaacgtcaa cattgtgatg aactatatta gaaacttgga ttctgaaact
    10621 ggcgccatgg ttcgtcttct cgaagacggt gattaagata aatgcaaaat cctttcaagc
    10681 attcccttct tctctatcac tattctttct ttttgtcaaa aaattctctc gctaatttat
    10741 ttgctttttt aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga
    10801 gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa
    10861 ttccattttg aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt
    10921 gtattgtgtt cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa
    10981 aagtctcact gaatctcaaa tattttatta gaaaacacca acaatttaga tatttctgta
    11041 tccacggttg gcgttgacag cctctgatgg tggagacttg tctgggcact ggcagtactc
    11101 ggcatccttt cctggtcttc cgtctggtcc agtctctccc tgggttccgt ctggtccttg
    11161 acgtccttgc tttccagatg gtccttctgg tccaacggtt cctggctctc ctggtggtcc
    11221 gtgaagaccg ttctgtccag ctggtcctgt tggtccttga tctcctcttg gtccctttgg
    11281 tcctggtctt ccgattggat gctcggcgtc gcgtcccttc tctcctggtg gtcctggctc
    11341 tccgatcttt ccgatttgac cggatgatcc ttgctctcct ggatgtcctg gttgtccatc
    11401 tcttcctggg tttccatttt gtcctcttgg tcctggaagt ccacgtggtc ctggtcttcc
    11461 aagagctcct ggtggtccaa ctggtccttg tgggcaagtg aagcatccga cagactcgcg
    11521 ttgtggagcg atatcatcag ctccaactcc tggaacaccg tcaagtcctg gaactccgtc
    11581 gagtcctgga actcctggaa ctccctttgg tccggatggt ccagctgggc acttgttatc
    11641 agcattgcag ttgcaggttg gtccatttcc attggctcca tttggagtct ttggctggtt
    11701 gaagactggt ggaactgctg gtggttgacc tggtggagca gatggggatc catcagcatg
    11761 tggagttggg gtagcgttgg tctcttcata ttgacaacgg acacgcttgc tagatcttcc
    11821 gatgacaact atgtccttcc acaatccatt ggtagattgc tagaaaatta aaaattattt
    11881 aaaattcaaa atataattta actcactctg aacacattca tctcggtatc aagcttctct
    11941 ctgaatccat caacctccga gtagatggtt gacatggtca tgaagcatac cataagagtg
    12001 gcgacagtga ccgaagccgt cacataacac gcaagtttta cagacatctc tgaaattatc
    12061 aaatttagga tttcaaaata attcggccaa agaaatgtaa ctcacctagt cttcgagaag
    12121 cagagacttg tcgatttcct accggattcg tctcctttta ttaggaaatc tttccgcaag
    12181 ttttcaagtt ttttttgttt tttttttaca ttaccgacga gacagtgtgc gagacgcaga
    12241 gacgataaaa gactggataa gaacgggaga taaggagtac ggatgtaggt gaggaatgtg
    12301 gccgaaagga cagatgggct ttattcggga agattggttg ggaaatgaac cagagaagaa
    12361 tgactgaata aagttgttga aacttggtga gaatggaaga tagacaatcg agggcagata
    12421 acttcgtata aggtatacta tacgaagtta tacgaagtga gtttgctacc atcatggaat
    12481 caggaggaca tttcgacaat gtcatataat gtttgaaaaa tgattttaac aagggtctca
    12541 ttcgagataa gcaatgctct gagcaagtgg ctcgtcgtct tcaagaatct cttgttgata
    12601 ttcatttgga tcctgttttg cacgaagcat gtgccatgga tattcagagt ttttgcaaaa
    12661 aagttccgcc aggtcaaaca atctttttaa tttttatatg atacttcggg tgtatttttc
    12721 ttctttttcc tgttcatttt cgcttttttc tcactttttt gtaacaaaat ctctcacttt
    12781 tctgtaagtc ttaacaaaag ttcaattggc tcgatcaagt aaaatttcgg ccgtatgaac
    12841 gttttcaaag gttttcattt ttttttaagt tcattcacaa tatcatgaat ctctcgtcct
    12901 ttggtttccg gtaagtagcg aaataatata agcatactta taaatgaagg aattgaatat
    12961 agaataagga aagcataact accaataacg gaatatagag gtaatactgt aaatgtactg
    13021 ataaccacca ttacagtatt cattgaatat gccacagatt gagcaatact acgatgtttt
    13081 tgaggtacca gttcagagga tataaaccac gaaattggac caactccagt tctgaaattt
    13141 cattattcga ttaaatcaaa atatatttga aaaacctcac ccataagtat aaccgtagat
    13201 gatcaatgca actaggcatc cgtatttcat cgggtcaatc agaatacgta tttttgcgaa
    13261 gaatacgaat gcaattagag ctaaattatt gagaaatgtg aagagaagga gaagcttgcg
    13321 tcgaccaact ctgtaaactt attcatacaa ttttgtcttc aaattttgaa cgtttaaaac
    13381 tatcttattc aatgagttag cgggttttta tagtgagtta gttcacgatt atctaataaa
    13441 actatgtcca aacatcctaa ctgcgaccgt attctcacta actcgtctta aaaacgttga
    13501 gattatttta aacccttctt cagtacaact acagtactga tgcacaaata caataataat
    13561 aatacattca gacatactct gaacaatgta acttgttttt gttatactgt ggtaacattt
    13621 attgaaatga aatataacct tacttttcaa tcacaattcc accagtaatt gttccagaaa
    13681 cataagctaa acccattgca gttgtactcc attcagcaat ttctgactca acatttgcat
    13741 tttctagaaa gaaagtagag gataaaagaa gagcccataa tccgacaggg ccctgtgaaa
    13801 ttgttatccg ctggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    13861 ggataacgca ggaaagaaca tgtgagcaaa aggcca
//
