LOCUS       sequence-34356-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-Tet SMR5970
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..30
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        48..66
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     48..65
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        109..393
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     316..335
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cggaacattt ccacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac
       61 tatagaatac tcaagcttgc atgacaggag ctctcccgcc attcagagaa gaaaccaatt
      121 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
      181 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
      241 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
      301 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
      361 gctttttatc gcaactctct actgtttctc catacccgtt ttaggaggta ctgagcatgc
      421 gtctagagct cgaggtagat ctgaaagctt gaatcgatgt acgtactata ccggtgcgaa
      481 cgggaaggta aaaagacaaa aagttgtttt taataccttt aagtgatacc agatggcatt
      541 gcgccatctg gcagagtgat taactaaaca tcgcagtaat cgaggcgctt gccagagagt
      601 ggaaatgaac gttaaacccg accatcgcgc cgctggcacc ttcatcgaca tcaatacgtt
      661 ctatatccag cgcgtgaacg gtaaaaatgt agcgatgagt ttcgcctttc ggcggtgctg
      721 cgccatcgta cccggtttta ccaaagtcgg tacgcgtctg caaaacgccg tctggcattg
      781 ct
//
