LOCUS       sequence-343576-        8516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: eSpCas9-EF
FEATURES             Location/Qualifiers
     source          1..8516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             781..828
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(856..873)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    862..1069
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1078..1218
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1078..1207
                     /label=AAV2 ITR
     rep_origin      1293..1748
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1380..1399)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1590..1611
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1765..1784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1884..1906
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1944..1962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2030..2134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2135..2203,2204..2995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2353..2372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3166..3754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3655..3674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3816..4064
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3816..3836
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3987..4006
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        4265..4550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        4552..4829
                     /label=chicken beta-actin promoter
     intron          4830..5058
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      5070..5079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5079..5144
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5151..5171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5196..7733,7734..7736,7737..8198,8199..8201,8202..8369,
                     8370..8372,8373..8516,1..780)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
       61 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
      121 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
      181 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
      241 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
      301 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
      361 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
      421 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
      481 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
      541 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
      601 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
      661 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
      721 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
      781 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagta agaattccta
      841 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      901 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      961 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     1021 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag cggccgcagg
     1081 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     1141 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     1201 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     1261 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     1321 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1381 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1441 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1501 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     1561 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     1621 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     1681 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     1741 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     1801 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1861 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1921 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1981 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2041 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2101 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2161 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2221 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2281 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2341 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2401 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2461 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2521 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2581 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2641 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2701 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2761 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2821 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc
     2881 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2941 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3001 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3301 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3781 gttcctggcc ttttgctggc cttttgctca catgtgaggg cctatttccc atgattcctt
     3841 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     3901 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     3961 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     4021 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct tcgagaagac
     4081 ctgtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     4141 acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta gagctagaaa tagcaagtta
     4201 aaataaggct agtccgtttt tagcgcgtgc gccaattctg cagacaaatg gctctagagg
     4261 tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     4321 cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4381 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     4441 ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg
     4501 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     4561 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     4621 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc
     4681 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     4741 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     4801 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
     4861 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     4921 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa
     4981 gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct
     5041 gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag
     5101 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
     5161 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
     5221 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
     5281 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
     5341 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
     5401 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
     5461 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
     5521 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
     5581 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
     5641 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
     5701 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
     5761 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     5821 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     5881 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     5941 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     6001 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     6061 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     6121 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     6181 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     6241 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     6301 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     6361 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     6421 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     6481 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     6541 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     6601 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     6661 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     6721 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     6781 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     6841 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     6901 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     6961 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     7021 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     7081 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     7141 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     7201 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     7261 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     7321 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     7381 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     7441 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     7501 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     7561 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     7621 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     7681 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctggccgacg
     7741 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     7801 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     7861 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     7921 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     7981 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     8041 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     8101 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     8161 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctgc gctggaaagc gagttcgtgt
     8221 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     8281 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     8341 ttaccctggc caacggcgag atccggaagg cgcctctgat cgagacaaac ggcgaaaccg
     8401 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     8461 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagag
//
