LOCUS       sequence-343590-        9773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For introducing multiple copies of OsTIR1 with the piggyBac system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Xiang K, Bartel DP
  TITLE     The molecular basis of coupling between poly(A)-tail length and 
            translational efficiency.
  JOURNAL   Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493.
  PUBMED    34213414
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-07-2"; volume: "10. pii: 66493. doi"; pages: " 
            10.7554/eLife.66493"
COMMENT     Sequence Label: C278
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             24..1748
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             1776..1805
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1812..1841
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1848..1877
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1896..1925
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1980..1999)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2024..2072
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2303..2514
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2527..2795
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2829..3572
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             3573..3626
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3627..3646)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             3675..4478
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3738..3757)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4348..4367
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4571..4692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4608..4627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4662..4681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5455..5489
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5980..5996)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5980..5996)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5993..6015)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6004..6020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6028..6034,6035..6052,6053..6058))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6073..6094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6211..6228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6382..6970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6462..6481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7141..7932,7933..8001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7764..7783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8002..8106)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8132..8587)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8269..8290)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8481..8500
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8714..8736
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8728..8745
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8729..8745
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8875..8937
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        9386..9764
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    9394..9412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9430..9448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9466..9484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9502..9520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9538..9556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9574..9592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9610..9628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9673..9697
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctagcgaatt cgaatttgcc accatgacgt acttcccgga ggaggtggtg gagcacatct
       61 tcagcttcct gccggcgcag cgcgaccgca acacggtctc gctcgtctgc aaggtgtggt
      121 acgagatcga gaggctgagc cgccgcggcg tcttcgtggg caactgctac gccgtgcgcg
      181 ccggccgcgt cgccgcgcgg ttccccaacg tgcgggcgct cacggtgaag gggaagcccc
      241 acttcgccga cttcaacctc gtgccccccg actggggcgg ctacgcgggg ccgtggatcg
      301 aggcggccgc gaggggatgc cacggcctgg aggagctcag gatgaagcgg atggtggtgt
      361 ccgacgagag cctcgagctg ctggctcgct cgttcccgcg gttcagggct cttgttctta
      421 tcagctgcga ggggttcagc actgacgggc tagccgccgt cgcgagccat tgcaagcttc
      481 tgagggagtt ggatttgcag gaaaatgaag tggaggatcg agggcctagg tggctttcct
      541 gcttccctga ttcctgcaca tcacttgtct cattgaattt tgcctgcatc aaaggggagg
      601 ttaatgctgg ttcactggag agacttgtta gcaggtcccc aaacctgcgg agtttgaggc
      661 tgaatcgatc tgtatcggta gatacacttg caaagatact actgcgtacc cctaacttgg
      721 aggatttggg gacagggaat ttgacagatg acttccaaac tgagtcctac tttaagctta
      781 ccagtgctct ggagaaatgc aagatgttga ggagtttgtc tggattctgg gatgcttctc
      841 ctgtttgcct gtcatttatc taccccctgt gtgctcaact gacaggattg aacttgagct
      901 atgcacccac acttgatgct tctgacctta caaaaatgat tagccgctgt gtgaagctcc
      961 aacgcctttg ggtactggat tgtatctcgg acaaaggctt gcaagtggtg gcctccagtt
     1021 gcaaagactt gcaagaactc agggtatttc catcagattt ctacgtagct ggttattctg
     1081 cagtgacaga ggagggactt gttgcagtat ccttgggctg tccaaaactg aactcactac
     1141 tgtacttctg tcaccaaatg actaatgctg cactagttac tgtcgccaag aactgtccaa
     1201 atttcacacg attcagactt tgtattcttg agccagggaa gcctgatgtt gtgacaagcc
     1261 aaccattaga tgaaggcttt ggagctattg ttcgtgagtg caagggatta caacgtttgt
     1321 caatatctgg tcttctcaca gacaaagttt tcatgtatat tgggaaatat gcaaaacaac
     1381 ttgagatgct ttctatagca tttgctggtg acagtgataa gggtatgatg catgttatga
     1441 atggatgcaa gaatttaagg aaactggaga taagagatag cccgtttggt gatgctgcac
     1501 tcttggggaa ttttgctagg tacgagacaa tgcgatccct ttggatgtca tcttgcaatg
     1561 tcacgttaaa ggggtgccaa gtccttgcgt caaagatgcc gatgctcaat gttgaggtca
     1621 taaatgagcg ggatggtagc aatgaaatgg aggaaaacca tggagatctg cctaaagtgg
     1681 agaaattata tgtgtaccgc acaactgctg gggcgaggga tgatgcacca aattttgtta
     1741 aaatcctagt cgactccggt tctgctgcta gtggtgaaca aaagttgatt tctgaagaag
     1801 atttgaacgg tgaacaaaag ctaatctccg aggaagactt gaacggtgaa caaaaattaa
     1861 tctcagaaga agacttgaac ggatcctcta gaggtgaaca aaagttgatt tctgaagaag
     1921 atttgtgaaa gggttcgatc cctaccggtt agtaatgagt ttaaacgggg gaggctaact
     1981 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     2041 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     2101 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     2161 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     2221 ggcaggccct gccatagcag atctgcgaaa tcggatccag ctgaatctaa gtggatctgc
     2281 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     2341 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     2401 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     2461 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     2521 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     2581 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     2641 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     2701 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     2761 tttgtttcgt tttctgttct gcgccgttac agatctaagc tgtgaccggc gcctactcta
     2821 gagccgccat gtctagactg gacaagagca aagtcataaa ctctgctctg gaattactca
     2881 atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     2941 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     3001 agatgctgga caggcatcat acccactcct gccccctgga aggcgagtca tggcaagact
     3061 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     3121 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     3181 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     3241 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     3301 gagagacacc taccaccgat tctatgcccc cacttctgaa acaagcaatt gagctgttcg
     3361 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     3421 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     3481 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     3541 ttgacgattt tgaccttgac atgctccccg gggagggcag aggaagtctt ctaacatgcg
     3601 gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca
     3661 cccgcgacga cgtcatggga tcggccattg aacaagatgg attgcacgca ggttctccgg
     3721 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     3781 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     3841 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     3901 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3961 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4021 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4081 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4141 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4201 ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct
     4261 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4321 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4381 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4441 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaga cgtcgaggtg cccgaaggac
     4501 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa
     4561 tttgtgggta aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4621 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4681 caatgtatct tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct
     4741 catctggtct cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc
     4801 ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc
     4861 acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga
     4921 ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg
     4981 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     5041 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     5101 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     5161 tttggggggg ggctgtccct gatatctata acaagaaaat atatatataa taagttatca
     5221 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     5281 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     5341 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     5401 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     5461 tcaattttac gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg
     5521 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
     5581 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact
     5641 gctgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
     5701 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc
     5761 ggcttttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
     5821 ccggcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
     5881 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac
     5941 cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6001 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6061 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6121 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6181 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7441 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7741 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8101 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     8161 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     8221 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     8281 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     8341 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8401 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8461 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8521 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8581 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8641 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8701 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc
     8761 attcacgttt ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc
     8821 gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag
     8881 aaagataatc atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt
     8941 tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat
     9001 atttacactt acatactaat aataaattca acaaacaatt tatttatgtt tatttattta
     9061 ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca
     9121 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     9181 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     9241 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     9301 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact
     9361 agtctttcgt cttcaagaat tcctcgagtt tactccctat cagtgataga gaacgtatga
     9421 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     9481 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     9541 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     9601 ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc
     9661 ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact
     9721 tttgtcttat accaactttc cgtaccactt cctaccctcg taaaggtcta gag
//
