LOCUS       sequence-343606-        3766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry red fluorescent protein for N-terminal tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pmCherry TL1
FEATURES             Location/Qualifiers
     source          1..3766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      373..382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(412..431)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(413..431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(438..455)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(438..454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..469)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      595..1050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(682..701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     892..913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1128..1232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1233..1301,1302..2093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1451..1470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2264..2852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2753..2772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3006..3023
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3140..3161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3176..3181,3182..3199,3200..3206)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3214..3230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3219..3241
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3238..3254
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3238..3254
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3272..3290
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3272..3289
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cggtcaccac tccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccttcgggga
       61 agctcagctt caggtagtcg ggaatgtcgg ccgggtgctt cacgtaggcc ttggagccgt
      121 acatgaactg ggggctcaag atgtcccagg caaagggcaa cgggcctccc ttggtcacct
      181 tcagtttggc ggtctgggtg ccttcgtagg gacgtccctc gccttcgccc tcgatctcga
      241 actcgtggcc attcacgctg ccctccatgt ggaccttgaa gcgcatgaac tccttgatga
      301 tggccatgtt atcctcttct cccttggaca ccattcgaga ccactcatcg cccaatcgaa
      361 ttcccgcggc cgccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt
      421 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      481 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      541 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
      601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccagcgc
      841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1021 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     1081 gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1201 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1261 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1321 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1381 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1441 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1501 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1561 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1621 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1681 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1741 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1801 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1861 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1921 ctgataaatc tggagccggt gagcgtgggt cgcgcggtat cattgcagca ctggggccag
     1981 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2041 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2881 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2941 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3001 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3061 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3121 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     3181 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     3241 gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc
     3301 tatgcatcca acgcgttggg agctctccca tatggtcgac ctgcaggcgg ccgcgaattc
     3361 actagtgatt gggcgatgag tggtctcgca ctcttgtaca gctcatccat gccgcccgtg
     3421 ctatgacgtc cttcggcacg ctcgtactgc tccacgatgg tgtagtcctc gttgtggctg
     3481 gtgatgtcca gcttgatgtt cacgttgtag gcgccgggca attgcacggg cttcttggcc
     3541 ttgtaggtgg tcttcacctc ggcgtcgtag tggcctccat ccttcagctt caggcgctgc
     3601 ttgatctcgc ccttcagagc gccgtcctcg ggatacatgc gctcgctgga agcctcccag
     3661 cccatggtct tcttctgcat caccgggcca tcggagggaa agttcgtgcc gcgcagcttg
     3721 accttgtaga tgaactcgcc gtcctgcaag ctggagtcct gggtca
//
