LOCUS       sequence-343610-       15339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15339)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 15339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-jGCaMP8s
FEATURES             Location/Qualifiers
     source          1..15339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3314..3331
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3613..3634
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3740..3761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4001..4020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             6754..6765
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8557..8568)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11764..11782)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11778..11825
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11853..11869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11853..11869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11866..11888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11877..11893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11901..11907,11908..11925,11926..11931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11946..11967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12084..12101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12255..12843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12335..12354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13014..13805,13806..13874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13637..13656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13875..13979)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14006..14462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14093..14112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14303..14324
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14588..14610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14602..14619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14603..14619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14689..14992
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14993..15196
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15147..15167
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15241..15260
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15241..15259
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaccagagaa gaaaaagaca gcgtcaattg caaagcaaaa atgtaacacc cctacaatgg
       61 atgccgacaa gattgtgttc aaagtcaata atcaggtggt ctctttgaag cctgagatta
      121 tcgtggatca atatgagtac aagtaccctg ccatcaagga tttgaaaaag ccttgtatca
      181 ccctagggaa agcccccgac ttgaacaaag catacaaatc agttttatca ggcatgaatg
      241 ccgccaaact tgatccggat gatgtatgct cctacttggc agcagcaatg cagttctttg
      301 aggggacatg tccggaagac tggaccagct atggaatcct gattgcacga aaaggagata
      361 ggatcacccc aaactctcta gtggagataa agcgtactga tgtagaaggg aattgggctc
      421 tgacaggagg catggaattg acaagggacc ccactgtctc tgaacatgca tctttagtcg
      481 gtcttctcct gagtctgtac aggttgagca aaatatcagg acagaacact ggtaactata
      541 agacaaacat tgcagatagg atagagcaga ttttcgagac agcacctttt gttaagatcg
      601 tggaacacca taccctaatg acaactcaca agatgtgtgc taattggagt actataccga
      661 acttcagatt tttggccgga acctacgaca tgtttttctc acggattgag catctatatt
      721 cggcaatcag agtgggcaca gtcgtcaccg cttatgaaga ctgctcagga ctggtatcgt
      781 ttacagggtt cataaagcag atcaatctca ccgcaaggga agcaatacta tatttcttcc
      841 acaagaactt tgaggaagag ataagaagaa tgttcgagcc agggcaagag acagctgttc
      901 ctcactctta tttcatccac ttccgttcac taggcttgag tgggaagtct ccttattcat
      961 cgaatgctgt cggtcatgtg ttcaatctca ttcactttgt tggatgctac atgggtcaag
     1021 tcagatctct aaatgcgacg gttattgctg catgtgcccc tcatgagatg tctgttctag
     1081 ggggctattt gggagaggaa ttcttcggaa aagggacatt tgaaagaagg ttcttcagag
     1141 acgagaaaga acttcaagaa tatgaggcgg ctgaactaac aaagtccgac gtggcactgg
     1201 cagatgacgg aaccgtcaac tctgatgacg aggactattt ctctggtgaa accagaagtc
     1261 cagaagctgt ctatactcga atcatgatga atggaggtcg actgaagaga tctcatatac
     1321 ggagatatgt ctcagtcagt tccaatcatc aagcccgtcc aaactcattc gccgaatttt
     1381 taaacaagac gtattcgaat gactcataag gagttgattg acagggtgcc agaaatctat
     1441 agattgtata tatccatcat gaaaaaaact aacactcctc ctttcaaacc atcccaaata
     1501 tgagcaagat ctttgttaat ccgagtgcaa tcagagccgg tctggccgat cttgagatgg
     1561 ccgaagagac tgttgatctg atcaacagaa acatagaaga caatcaggct catctccagg
     1621 gagaacccat agaagtggac aacctccctg aggacatgaa gcgacttcac ctggacgatg
     1681 aaaaatcgtc caaccttggt gagatggtta gggtgggaga aggcaagtat cgagaggact
     1741 ttcagatgga tgagggagag gaccccaacc tcctgttcca atcgtacctg gataatgttg
     1801 gagtccaaat agtcagacaa atgaggtcag gagagagatt cctcaagata tggtcacaga
     1861 ccgtagagga aattgtatcc tatgtcacgg tcaactttcc taaccctcca agaaggtctt
     1921 cggaggataa atcaacccag actactggca gagagctcaa gaaggagaca acgtctgctt
     1981 tctctcagag agaaagccaa ccttcgaaag ctaggatggt ggctcaagtt gcccctggtc
     2041 ctccagccct tgaatggtca gccaccaatg aagaagatga tctatcagta gaggctgaga
     2101 tcgctcatca gattgctgaa agcttttcca agaagtacaa gtttccctcc cgatcttcag
     2161 gaatattctt gtataatttt gagcaactga agatgaacct tgatgacata gttaaagagg
     2221 caaaaaatgt accgggcgtg acccgtctgg cccatgatgg atccaaaatc cccctgagat
     2281 gcgtactggg atgggtcgct ttggctaatt ccaaaaaatt ccaattacta gtcgaggctg
     2341 acaagctaag caaaatcatg caagatgatt tgaatcgcta tacatcctgc taaccgagtt
     2401 ttcgaactca gtccctccag ataatgaaaa ctgagatgtt atggagtaga catgaaaaaa
     2461 acaggcaaca ccactgataa aatgaacgtt ctacgcaaga tagtgaaaaa atgtagggat
     2521 gaggacactc aaaagccctc tcctgtgtca gcccctccgt atgacgatga cctgtggctt
     2581 ccacctcctg aatatgtccc gctgaaagaa ctcacaagca agaagaacat gaggaacttt
     2641 tgtgtcaacg gggaggttaa agcgtgtagc ccaaatggtt actcattcag gattttgcgg
     2701 cacattctga gatcattcaa cgagatatac tctgggaatc ataggatgat tgggttagtc
     2761 aaaattgttg ttggactagc tttatcagga gctccagtac ctgagggcat gaactgggta
     2821 tacaaattga ggagaaccct tatattccag tgggctgatt ccaggggccc tcttgaaggg
     2881 gaggagttag attactctca agagatcact tgggatgatg atactgaatt cgtcggattg
     2941 caaataagag tgagcgcaag acaatgtcat attcaaggca ggatctggtg tatcaacacg
     3001 aactcgaggg catgtcaact atggtctgac atgtctcttc agacacaaag gtctgaagag
     3061 gacaaagact cttctctgct tctagaataa tcagattata tcctgcaagt gtatcacttg
     3121 tttacctctg gaggagagag catacaggct tgactccgat ccttgggagc aatagaacaa
     3181 aaaaacacac gttatggtgc cgttaaatcg ctgcatttta tcaaagtcaa gttgataacc
     3241 tttacatttt gagcctcttg gatgtgaaaa aaactattaa catccctcaa aagaccccgg
     3301 gaaagccacc atgcatcatc accatcatca cacgcgtcgc aagaagacct tcaaggaggt
     3361 ggccaccgcc gtgaagatca tcgccatgct gatgggcctg aagatcaacg tctatatcaa
     3421 ggccgacaag cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga
     3481 cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt
     3541 gctgctgccc gacaaccact acctgagcgt ggagtccaaa ctttcgaaag accccaacga
     3601 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3661 ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg aggagctgtt
     3721 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3781 cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3841 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3901 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3961 gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4021 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4081 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc tgccggacca
     4141 actgactgaa gagcagatcg cagaatacaa agaggctttc tccctatttg acaaggacgg
     4201 ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctcg gacacaaccc
     4261 cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacaat
     4321 cgacttccct gagttcctga caatgatggc aagaaaaatg aaatacaggg acacggaaga
     4381 agaaattaga gaagcgttcg gtgtgtttga taaggatggc aatggctaca tcagtgcagc
     4441 agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag aggttgatga
     4501 aatgatcagg gaagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca
     4561 aatgatgaca gcgaagtaag ctagcggcgc ctagccggtc atccttttga cgattccagt
     4621 cccgaggata acctcctctc gggattgggg ggaatctttg gatccagtag tcctccttga
     4681 actccgtcca acagggcaga ttcaagagtc ataagacttt cattaatcat ttcagttgat
     4741 cagacatggt cgtgtagatt ctcttaatac gggagatctt ctagcagttt cagtgaccaa
     4801 cggtgctttc attctccagg aactgatacc aaaggttgtg gacaggccaa ggggtacttc
     4861 ggatgactct gtgcttgggc acagaaagag gtcgtagtgt gccccccgat agcggactcg
     4921 acatgaatca actaagaaag gcaatctgcc tcccatgatg gacataagca atagttcaca
     4981 accatcttgc atctcagtga agtgtacata actattgagg gctgggtcat ctaagcattt
     5041 cagtcgagaa aaaaactgta gaccaaaaga acaactagca acacttctca tccagagacc
     5101 catatcaaga tgctagatcc gggagaggtt tatgatgacc ctattgatcc aattgagtca
     5161 gaggctgaac ccagaggaac ccccactgtc cccaacatct tgaggaactc cgactacaat
     5221 ctcaattctc ctttgataga ggatcctgcc aaactaatgt tagaatggtt gaagacaggg
     5281 aacagacctt atcggatgac tttgacagac aattgctcca ggtcttacaa agttttgaaa
     5341 gattatttca agaaagtaga tttgggttct ctcaaagtgg gcggaactgc tgcacagtca
     5401 atggtttctc tctggttgtg tggtgcccac tctgaatcaa acaggagccg gagatgtata
     5461 accgacttgg cccatttcta ttccaagtca tcccccatag agaagctatt gaattgtacg
     5521 ctaggaaaca gaggcctgag aatcccacca gagggggtgt taaattgcct cgagagggtc
     5581 aattatgaca aggcatttgg gaggtatctg gccaacacgt attcctctta cttgtttttc
     5641 catgtaatca ccttatacat gaatgcctta gactgggaag aggaaaagac catcctggca
     5701 ttatggaaag atataacctc agtggatacc gagaaggact tggtcaaatt caaagatcaa
     5761 atatggggac tgttgattgt gacaaaggac tttgtttact ctcagagttc aaactgtctt
     5821 tttgacagaa actacacact gatgctaaag gatcttttct tgtctcgatt caactcctta
     5881 atgattctgc tttctccccc tgagccccga tactcagatg acttaatatc tcagctgtgc
     5941 cagctataca tcgctgggga tcaagtcttg tccatgtgtg ggaactccgg ctatgaagtc
     6001 atcaaaatat tggagccata tgtcgtgaac agtttggtcc agagggcaga gaagtttagg
     6061 cctctcatcc accccttggg agactttcct atgttcataa aagacaaggt gaatcaactt
     6121 gaagggactt ttggtcccag tgcaaagagg ttttttaggg ttttagatca attcgacaac
     6181 atacatgact tagtatttgt gtatggctgt tacagacatt gggggcaccc ctatatagat
     6241 tatcggaagg gtctgtcgaa actatatgat caagttcaca ttaagaaagt aatagataag
     6301 tcctaccagg agtgtttagc aagtgacttg gccagaagga tcctcagatg gggatttgac
     6361 aagtactcca aatggtatct agattcgaga ttccttgccc gagaccaccc cttgactcct
     6421 tatgtcaaga cccaaacatg gccacccaaa catatagtag acttggtggg ggacacatgg
     6481 cataagctcc cgatcacgca gatctttgaa attcctgaat caatggaccc gtcagagata
     6541 ctggatgaca aatcacattc tttcaccaga acaaggttag cttcttggct gtcagagaac
     6601 cgaggggggc ctgttcctag cgagaaggtc attatcacgg ccctgtctaa gccacctgtc
     6661 aatccccgag agtttttgaa atctatagac ctcggaggat tgccagatga ggatttgata
     6721 attggcctca aaccaaagga acgggagttg aagatcgagg gccgattctt tgctctaatg
     6781 tcatggaatc taagattata ttttgtcatc accgaaaagc tcttggccaa ctacattttg
     6841 ccactttttg acgcactgac tatgacagac aacctgaaca aggtgttcaa aaagttgatc
     6901 gacagggtca ccgggcaagg gcttttggac tattctaggg tcacatacgc atttcacctg
     6961 gactatgaga aatggaacaa tcatcaaaga ttggagtcaa cagaggatgt attctctgtc
     7021 ctagatcagg tgtttggatt gaagagggtg ttttctagaa cacacgagtt ttttcagaag
     7081 tcctggatct attattcaga cagatcagac ctcattgggt tatgggagga ccaaatatat
     7141 tgcttggata tgtctaacgg cccaacctgc tggaatggcc aagatggcgg gctagagggc
     7201 ttgcggcaga agggctggag tctagtcagt ttattaatga tagatagaga atctcaaacc
     7261 aggaacacaa gaaccaagat actagctcaa ggagacaacc aggttctgtg tcctacatac
     7321 atgttgtcac cgggattgtc tcaagagggg cttctctatg agttagagag catatcaagg
     7381 aatgcactct caatataccg agctatcgag gaaggagcat ctaagctggg gctgatcatc
     7441 aagaaggaag agaccatgtg tagttatgac tttctcatat atgggaagac ccccttattt
     7501 cgaggcaaca tattggtgcc tgaatccaaa agatgggccc gagtctcttg catctctaac
     7561 gaccaaatag tcaacctcgc caatataatg tcgacagtat ccaccaatgc gctgacagtg
     7621 gcacaacact ctcaatctct gatcaaacct atgagggatt ttctgctcat gtcagtacag
     7681 gcagttttcc actacctgtt gtttagccca atcctaaaag gcagagttta taagattctg
     7741 agtgctgaag gggagagctt tctcctagcc atgtcgcgga taatctacct agatccttct
     7801 ttgggagggg tgtctggaat gtctctcggg aggttccata tacgtcagtt ctcagaccct
     7861 gtctctgaag ggttgtcatt ctggagagag atctggttag gctctcatga gtcctggatt
     7921 cacgcgttgt gtcaggaggc cgggaacccc gatcttggag agagaacact cgagagcttc
     7981 actcgccttt tagaagatcc tactacctta aatatcaaag gaggggccag ccctaccatt
     8041 ctactcaagg atgctatcag aaaggctctg tacgacgagg tggacaaggt ggaaaattca
     8101 gagtttcgag aggcaatcct gctgtccaag acccatagag ataactttat actcttttta
     8161 aaatctgttg agcctctgtt ccctcgattt ctcagtgagc tcttcagttc gtctttcttg
     8221 ggaataccgg agtcaatcat tggactgata caaaactccc ggacaataag aaggcagttt
     8281 agaaagagtc tctcaagaac tttagaagag tccttctaca actcagagat ccacgggatt
     8341 aatcggataa cccagacacc tcaaagggtc ggaagggtgt ggccttgctc ttcagagagg
     8401 gcagatctac ttagggagat ctcttggggg aggaaagtgg taggcacgac agttcctcac
     8461 ccttccgaga tgttggagtt gtttcccaaa tcctccattt cctgcacttg tggagcaaca
     8521 gggggaggca atcctagagt ctctgtatca gtactcccgt ccttcgatca gtcatttttc
     8581 tcacggggcc ccctaaaggg atacttgggc tcgtccacct ccatgtcaac ccagctattc
     8641 catgcatggg aaaaagtcac taatgttcat gtggtgaaaa gggctctatc gttaaaagaa
     8701 tctataaact ggttcatcaa taggaattcc aatttggctc aaactctaat tggaaacatc
     8761 atgtctctga caggccctga tttccctcta gaagaggccc ctgttttcaa acggacaggg
     8821 tcagccttgc ataggttcaa gtctgccaga tacagcgaag gagggtattc ttctgtttgc
     8881 cctaaccttc tctcccatat ctctgttagt acagacacta tgtctgattt gacccaaaac
     8941 gggaagaact atgatttcat gtttcagcca ttgatgcttt atgcgcaaac atggacatcg
     9001 gaactggtac agagggatac aagacttaga gactccacgt ttcactggca ccttcggtgc
     9061 aacagatgtg tgaggcccat tgatgatata acactggaaa cttctcagat cttcgagttc
     9121 ccggatgtgt caaaaaggat atccaggatg gtttctggag ctgtccctca atttcagaag
     9181 cttcctgata tccgtctaag accaggtgat tttgaatccc taagtggtag agaaaagtct
     9241 cgccatatag ggtcagctca ggggctctta tactcaatct tagtagcaat tcacgactca
     9301 ggatacaatg atgggaccat cttccctgtc aacatatacg gcaaagtttc ccccagagac
     9361 tatttgagag ggcttgcaag agggatcttg atagggtcct cgatttgctt cttgacacga
     9421 atgacaaata ttaacattaa cagacctctt gaattgatct caggggtaat ttcctatatt
     9481 ctcctgaggc tggataatca tccctctctg tatataatgc ttagagaacc gtctcttaga
     9541 ggagaaatat tctctatccc tcagaaaatc cccgccgctt acccaaccac tatgagagaa
     9601 ggcaacagat cgattttgtg ttacctccaa cacgtgctac gctatgagcg agaggcaatc
     9661 acggcgtccc cggagaatga ctggctgtgg atcttctcag acttcagaag tgtgaaaatg
     9721 acgtacttga ccctcattac ctaccagtct cacctcctac tccagagggt tgagagaaac
     9781 ttgtctaaga gtatgagagc tactctgcga caaatgagtt ccttaatgag gcaagtgctg
     9841 ggtgggcacg gagaagatac cttggagtca gacgatgaca ttcaacgatt actaaaagac
     9901 tctttgcgaa agacaaggtg ggtggatcaa gaggtgcgcc atgcagctag aaccatgaat
     9961 ggagattaca gccccgacaa gaaagtatcc cacaaggcag gatgttcaga atgggtctgc
    10021 tctgctcaac agattgccgt ctccacctca gccaacccgg cccctgtctc agagcttgac
    10081 attagggccc tctctaagag gtttcaaaac cccttgatct cgggcctgag agtggttcag
    10141 tgggcaaccg gtgcccatta taagcttaag cctattctag atgatctaaa tgttttccca
    10201 tctctctgtc ttgtaattgg agacgggtca gggggaatat caagggcagt tctcaacatg
    10261 tttccagatt ctaagcttgt gttcaacagc ctattggagg tgaatgatct gatggcttcc
    10321 ggaacacatc cactgcctcc ttcagcaatc atgagtggag gagatgacat catctccaga
    10381 gtgatagact ttgactcaat atgggagaaa ccgtccgacc tgaggaactc ggccacctgg
    10441 agatacttcc agtcggttca aaaacaggtc aacatgtcgt atgacctcat tatttgtgat
    10501 gcagaagtta ctgatattgc atctatcaac cggataactc tgttgatgtc tgatttcgca
    10561 ttgtctatag atggaccact ttatctggtc ttcaaaactt acgggactat gctagtaaac
    10621 ccggactata aagctattca acatctgtca agagcgttcc cttcggtcac agggtttgta
    10681 acccaagtaa cttcatcctt ttcttctgag ctatacctcc ggttctctaa acgaggaaag
    10741 tttttcaggg acgccgagta cttgacctct tccacccttc gagagatgag ccttgtgttg
    10801 ttcaattgca gcagccccaa aagtgagatg cagagagctc gttccttaaa ctatcaagac
    10861 ctggtaaggg gatttcctga agagatcata tcaaatcctt acaacgaaat gatcataact
    10921 ctgattgaca atgatgtaga gtccttccta gtccacaaga tggtggatga tcttgagcta
    10981 cagaggggaa ctctgtctaa agtggctatc attatatcca tcatgatcgt tttttccaat
    11041 agagtcttca acatttccaa acctttgact gaccccttgt tctacccccc atctgatcct
    11101 aaaatcctga ggcacttcaa catatgttgc agtactatga tgtatctatc taccgcttta
    11161 ggcgacgtcc ctaacttcgc aagacttcat gacctgtata acagacctat aacttgttac
    11221 ttcagaaaac aagttattcg agggaatatt tatctatctt ggagttggtc cgatgatacc
    11281 ccagtgttca agagagtagc ctgtaattct agcttgagtc tgtcatctca ctggatcagg
    11341 ttgatctaca agatagtgaa gactaccaga ctcattggca gcataaaaga cctatcagga
    11401 gaggtagaac gacatcttca tgggtataac agatggatca ccctcgagga tatccgatct
    11461 agatcatccc tactagatta cagttgtttg taagccggat attaccgaaa gcctgtgcat
    11521 gctaaaattc ttgtatgatg catcttgaaa aaaacaagat cttgaatccg gacctctggt
    11581 tgtttgattg ttttttccat ctttattgtt tttttgttaa gcgtgggtcg gcatggcatc
    11641 tccacctcct cgcggtccga cctgggcatc cgaaggagga cgcacgtcca ctcggatggc
    11701 taagggaggg cggggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
    11761 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
    11821 ttttgctgaa agtcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    11881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    11941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    12001 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    12061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    12121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    12181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    12241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    12301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    12361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    12421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    12481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    12541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    12601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    12661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    12721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    12781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    12841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    12901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    12961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    13021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    13081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    13141 gcaatgatac cgcgagaccc acccttggag gctccagatt tatcagcaat aaaccagcca
    13201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    13261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    13321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    13381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    13441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    13501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    13561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    13621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    13681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    13741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    13801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    13861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    13921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    13981 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
    14041 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    14101 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    14161 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    14221 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    14281 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    14341 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    14401 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    14461 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    14521 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcga
    14581 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgccta gttattaata
    14641 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
    14701 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    14761 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    14821 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    14881 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    14941 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    15001 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    15061 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    15121 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    15181 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
    15241 taatacgact cactataggg agacccaagc tggctagatt aagcgtctga tgagtccgtg
    15301 aggacgaaac ccggcgtacc gggtcacgct taacaacaa
//
