LOCUS       sequence-343626-       10257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen
            C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, 
            Liu DR
  TITLE     Enhanced prime editing systems by manipulating cellular determinants
            of editing outcomes.
  JOURNAL   Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 
            10.1016/j.cell.2021.09.018.
  PUBMED    34653350
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09"; journalName: "Cell"; date: "2021-10-28- 
            28"; volume: "184"; issue: "22"; pages: "5635-5652.e29"
COMMENT     Sequence Label: pCMV-PEmax-P2A-BSD
FEATURES             Location/Qualifiers
     source          1..10257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     637..656
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        637..655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             705..725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             726..4826
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4884..4904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7005..7025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7035..7061
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7071..7127
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7128..7526
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             7532..7549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7572..7589)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7578..7802
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7873..7889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7873..7889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7886..7908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7897..7913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7921..7927,7928..7945,7946..7951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7966..7987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8104..8121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8275..8863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8355..8374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9034..9825,9826..9894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9657..9676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9895..9999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10078..10097)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
      601 agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact atagggagag
      661 ccgccaccat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag aagaagcgga
      721 aagtcgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg
      781 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      841 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      901 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      961 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
     1021 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
     1081 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
     1141 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
     1201 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
     1261 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
     1321 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
     1381 gaaagctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa
     1441 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     1501 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
     1561 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     1621 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
     1681 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     1741 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     1801 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     1861 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaagagagag gacctgctgc
     1921 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
     1981 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     2041 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     2101 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
     2161 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     2221 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     2281 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     2341 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     2401 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     2461 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     2521 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     2581 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     2641 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     2701 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     2761 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     2821 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     2881 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     2941 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     3001 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     3061 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     3121 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     3181 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg
     3241 ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     3301 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     3361 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     3421 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     3481 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     3541 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     3601 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     3661 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     3721 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     3781 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     3841 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     3901 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     3961 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     4021 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     4081 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     4141 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     4201 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     4261 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     4321 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac
     4381 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     4441 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     4501 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     4561 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     4621 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     4681 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca
     4741 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     4801 tcgacctgtc tcagctggga ggtgactccg gcggaagctc tggtggcagc aagcggaccg
     4861 ccgacggctc tgaattcgag agccctaaga agaaaagaaa ggtgagcgga ggctctagcg
     4921 gcggaagcac cctgaacatt gaagacgagt atagactgca tgaaacaagc aaggaacccg
     4981 acgtgtccct gggctccacc tggctgtccg actttcccca ggcctgggcc gagacaggag
     5041 gaatgggcct ggccgtgcgg caggcacccc tgatcatccc tctgaaggcc acctctacac
     5101 ccgtgagcat caagcagtac cctatgtctc aggaggccag actgggcatc aagcctcaca
     5161 tccagaggct gctggaccag ggcatcctgg tgccatgcca gagcccctgg aacacaccac
     5221 tgctgcccgt gaagaagcca ggcaccaatg actatagacc cgtgcaggat ctgagagagg
     5281 tgaacaagag ggtggaggat atccacccca ccgtgcccaa cccttacaat ctgctgtccg
     5341 gcctgccccc ttctcaccag tggtatacag tgctggacct gaaggatgcc ttcttttgtc
     5401 tgagactgca ccctaccagc cagccactgt tcgcctttga gtggagggac cctgagatgg
     5461 gcatctctgg ccagctgacc tggacacgcc tgcctcaggg cttcaagaat agcccaacac
     5521 tgtttaacga ggccctgcac cgcgacctgg cagatttccg gatccagcac ccagatctga
     5581 tcctgctgca gtacgtggac gatctgctgc tggccgccac cagcgagctg gattgccagc
     5641 agggaacacg cgccctgctg cagaccctgg gaaacctggg atatagggca tccgccaaga
     5701 aggcccagat ctgtcagaag caggtgaagt acctgggcta tctgctgaag gagggccaga
     5761 gatggctgac agaggccagg aaggagacag tgatgggcca gccaacaccc aagaccccaa
     5821 gacagctgag ggagttcctg ggcaaagcag gattttgcag gctgttcatc ccaggattcg
     5881 cagagatggc agcacctctg tacccactga ccaagccggg caccctgttt aattggggcc
     5941 ctgaccagca gaaggcctat caggagatca agcaggccct gctgacagca ccagccctgg
     6001 gcctgccaga cctgaccaag cctttcgagc tgtttgtgga tgagaagcag ggctacgcca
     6061 agggcgtgct gacccagaag ctgggaccat ggagacggcc cgtggcctat ctgtccaaga
     6121 agctggaccc agtggcagca ggatggccac catgcctgag gatggtggca gcaatcgccg
     6181 tgctgacaaa ggatgccggc aagctgacca tgggacagcc actggtcatc ctggcaccac
     6241 acgcagtgga ggccctggtg aagcagcctc cagatcgctg gctgtctaac gcccggatga
     6301 cacactacca ggccctgctg ctggacaccg atcgcgtgca gtttggccct gtggtggccc
     6361 tgaatccagc caccctgctg cctctgccag aggagggcct gcagcacaac tgtctggaca
     6421 tcctggcaga ggcacacgga acaaggccag acctgaccga tcagcccctg cctgacgccg
     6481 atcacacatg gtataccgat ggaagctccc tgctgcagga gggccagagg aaggcaggag
     6541 cagcagtgac cacagagaca gaagtgatct gggccaaggc cctgccagca ggcacatccg
     6601 cccagcgggc cgagctgatc gccctgaccc aggccctgaa gatggccgag ggcaagaagc
     6661 tgaacgtgta cacagactcc agatatgcct tcgccaccgc acacatccac ggagagatct
     6721 acaggcgccg gggctggctg acctctgagg gcaaggagat caagaacaag gatgagatcc
     6781 tggccctgct gaaggccctg tttctgccca agcggctgag catcatccac tgtcctggac
     6841 accagaaggg acactccgcc gaggcaaggg gcaatcggat ggccgaccag gccgccagaa
     6901 aggctgctat tactgaaact cccgacactt ccactctgct gattgaaaac tcctcccctt
     6961 ctggcggctc aaaaagaacc gccgacggca gcgaattcga gtctcccaag aagaagagga
     7021 aagtcggctc tggccctgcc gctaagagag tgaagctgga cggatccggc gcaacaaact
     7081 tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gccaagcctt
     7141 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     7201 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     7261 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     7321 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     7381 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     7441 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     7501 cctctggtta tgtgtgggag ggctaaccgg tcatcatcac catcaccatt gagtttaaac
     7561 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     7621 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagaa
     7681 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     7741 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7801 ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt
     7861 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7921 caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact
     7981 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8701 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8881 ttctacgggg tctgacactc agtggaacga aaactcacgt taagggattt tggtcatgag
     8941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10021 acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac
    10081 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    10141 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    10201 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccag
//
