LOCUS       sequence-343650-       19932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR dCas9 directly fused to a variant of bacterial DNA 
            methyltransferase MQ1 to target CG specific methylation to the FWA 
            locus with three guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19932)
  AUTHORS   Ghoshal B, Picard CL, Vong B, Feng S, Jacobsen SE
  TITLE     CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by
            a bacterial CG-specific DNA methyltransferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2125016118. doi: 
            10.1073/pnas.2125016118.
  PUBMED    34074795
REFERENCE   2  (bases 1 to 19932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-8"; volume: "118(23):e2125016118. 
            doi"; pages: " 10.1073/pnas.2125016118"
COMMENT     Sequence Label: pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
FEATURES             Location/Qualifiers
     source          1..19932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        1..76
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        161..506
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     449..469
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             579..1604
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1645..1819
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1909..1933
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2358..3152
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4577..5165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5066..5085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5319..5336
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5351..5491
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5417..5436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5577..5599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5835..6029
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6095..7168)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7597..8226)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9527..9551
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9739..9761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9753..9770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9754..9770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9794..9812
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9794..9811
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(9845..10552)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(11747..11767)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(11780..13294,13295..13297,13298..13363,
                     13364..13369,13370..15853,15854..15856,15857..15883))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(11780..13363,13364..13366,13367..15853,
                     15854..15856,15857..15883))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(11780..15883)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(15893..15913)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(15920..15985)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    15999..16054
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    complement(16073..16097)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             18808..18969
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             18997..19047
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     misc_RNA        19798..19873
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
       61 ggcaccgagt cggtgctttt tttggtacca tggtggagca cgacactctc gtctactcca
      121 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
      181 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
      241 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
      301 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
      361 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
      421 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
      481 gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat
      541 ctctctcgag ctttcgcaga tccggggggc aatgagatat gaaaaagcct gaactcaccg
      601 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
      661 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
      721 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
      781 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagtttagc gagagcctga
      841 cctattgcat ctcccgccgt tcacagggtg tcacgttgca agacctgcct gaaaccgaac
      901 tgcccgctgt tctacaaccg gtcgcggagg ctatggatgc gatcgctgcg gccgatctta
      961 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     1021 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     1081 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     1141 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     1201 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     1261 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     1321 acttcgagcg gaggcatccg gagcttgcag gatcgccacg actccgggcg tatatgctcc
     1381 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     1441 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     1501 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     1561 gtggaaaccg acgccccagc actcgtccga gggcaaagaa atagagtaga tgccgaccgg
     1621 gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa
     1681 gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta
     1741 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     1801 cagtactaaa atccagatcc cccgaattag agctcgaatt aattcggcgt taattcagta
     1861 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     1921 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     1981 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     2041 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     2101 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     2161 gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
     2221 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
     2281 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
     2341 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     2401 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     2461 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     2521 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     2581 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
     2641 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
     2701 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
     2761 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
     2821 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
     2881 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
     2941 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
     3001 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
     3061 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     3121 aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt
     3181 aacgctgaga gatcccctca taatttcccc aaagcgtaac catgtgtgaa taaattttga
     3241 gctagtaggg ttgcagccac gagtaagtct tcccttgtta ttgtgtagcc agaatgccgc
     3301 aaaacttcca tgcctaagcg aactgttgag agtacgtttc gatttctgac tgtgttagcc
     3361 tggaagtgct tgtcccaacc ttgtttctga gcatgaacgc ccgcaagcca acatgttagt
     3421 tgaagcatca gggcgattag cagcatgata tcaaaacgct ctgagctgct cgttcggcta
     3481 tggcgtaggc ctagtccgta ggcaggactt ttcaagtctc ggaaggtttc ttcaatctgc
     3541 attcgcttcg aatagatatt aacaagttgt ttgggtgttc gaatttcaac aggtaagtta
     3601 gttgctagaa tccatggctc ctttgccgac gctgagtaga ttttaggtga cgggtggtga
     3661 caatgagtcc gtgtcgagcg ctgatttttt cggcctttag agcgagattt atacaataga
     3721 atttggcatg agattggatt gcttttagtc agcctcttat agcctaaagt ctttgagtga
     3781 ctagatgaca tatcatgtaa gttgctgata ggtttccagt tttccgctcc taggtctgca
     3841 tattgtactt ttcctcttac tcgacttaac cagtaccaac ccagcttctc aacggattta
     3901 taccatggca ctttaaagcc agcatcactg acaatgagcg gtgtggtgtt actcggtaga
     3961 atgctcgcaa ggtcggctag aaattggtca tgagctttct ttgaacattg ctctgaaagc
     4021 gggaacgctt tctcataaag agtaacagaa cgaccgtgta gtgcgactga agctcgcaat
     4081 accataagcc gtttttgctc acggatatca gaccagtcaa caagtacaat gggcatcgta
     4141 ttgcccgaac agataaagct agcatgccaa cggtatacag cgagtcgctc tttgtggagg
     4201 tgacgattac ctaacaatcg gtcgattcgt ttgatgttat gttttgttct cgctttggtt
     4261 ggcaggttac ggccaagttc ggtaagagtg agagttttac agtcaagtaa ggcgtggcaa
     4321 gccaacgtta agctgttgag tcgttttaag tgtaattcgg ggcagaattg gtaaagagag
     4381 tcgtgtaaaa tatcgagttc gcacattttg ttgtctgatt attgattttt ggcgaaacca
     4441 tttgatcata tgacaagatg tgtatctacc ttaacttaat gattttgata aaaatcatta
     4501 ggggattcat cagcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4561 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4621 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4681 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4741 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4801 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4861 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4921 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4981 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5041 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5101 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5161 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5221 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5281 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5341 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5401 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     5461 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     5521 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5581 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     5641 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     5701 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     5761 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     5821 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     5881 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     5941 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     6001 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     6061 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     6121 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     6181 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     6241 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     6301 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     6361 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     6421 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     6481 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     6541 ccttctttgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     6601 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     6661 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     6721 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     6781 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     6841 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     6901 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     6961 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     7021 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     7081 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     7141 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     7201 attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     7261 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     7321 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     7381 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     7441 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     7501 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     7561 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     7621 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     7681 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     7741 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     7801 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     7861 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
     7921 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
     7981 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
     8041 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
     8101 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
     8161 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
     8221 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
     8281 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
     8341 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
     8401 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
     8461 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
     8521 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
     8581 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
     8641 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
     8701 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
     8761 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
     8821 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
     8881 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
     8941 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
     9001 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
     9061 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
     9121 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
     9181 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
     9241 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
     9301 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
     9361 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
     9421 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
     9481 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
     9541 atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc
     9601 tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     9661 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     9721 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg
     9781 catgcctgca gagatttagg tgacactata gaatatcact agtaagctag cttgcatgcc
     9841 ggtcctgctg agcctcgaca tgttgtcgca aaattcgccc tggacccgcc caacgatttg
     9901 tcgtcactgt caaggtttga cctgcacttc atttggggcc cacatacacc aaaaaaatgc
     9961 tgcataattc tcggggcagc aagtcggtta cccggccgcc gtgctggacc gggttgaatg
    10021 gtgcccgtaa ctttcggtag agcggacggc caatactcaa cttcaaggaa tctcacccat
    10081 gcgcgccggc ggggaaccgg agttcccttc agtgagcgtt attagttcgc cgctcggtgt
    10141 gtcgtagata ctagcccctg gggcactttt gaaatttgaa taagatttat gtaatcagtc
    10201 ttttaggttt gaccggttct gccgcttttt ttaaaattgg atttgtaata ataaaacgca
    10261 attgtttgtt attgtggcgc tctatcatag atgtcgctat aaacctattc agcacaatat
    10321 attgttttca ttttaatatt gtacatataa gtagtagggt acaatcagta aattgaacgg
    10381 agaatattat tcataaaaat acgatagtaa cgggtgatat attcattaga atgaaccgaa
    10441 accggcggta aggatctgag ctacacatgc tcaggttttt tacaacgtgc acaacagaat
    10501 tgaaagcaaa tatcatgcga tcataggcgt ctcgcatatc tcattaaagc aggactctag
    10561 ttatcatggc gcgctagcta gggccgccga tcttgtcaat gatggcctcc agcacctcca
    10621 cgctgatgct gttgccacac acgaagatct tctggttctc ggtcaggaac tcgatttcgt
    10681 tcactctctt gccgtcctgg ctgtcaaagc cgatgtacag gaaggtctcg tcgctgttca
    10741 tcttcctgat gttgctgccg tccttgatct tgatcctgga gttggcgccg ctagctgtca
    10801 gtgtggggcc tgtgaactcg gggtcgtaca catagccctc ggagttgaac ttggagtagc
    10861 cgatcaggct ggccttgttg atgttggact tggtcttctt gaattcggtg aggttgtatt
    10921 tcagcaggtt gttgaggatg tccttctcgc tcacgatctt gttcagcacc tttttgatgc
    10981 tcttgggctt cttgtcgccc ttaggcagtt ccacgaactc attgagggtg gagatcatga
    11041 acactctcct cctggcctgg ctggatccga aatcggcagc attgaggact tcgatgctgt
    11101 tctggtagcc caggctctcc agcttctgct tccattggtt cagctcctcc tcgttcttct
    11161 tgtgcagcag ggctcccacg ttctccatga ggaggtactt aggcaggtcg ttcttctcgg
    11221 tggagtccag ggctctttcg atctcccaga gcaggccgga tctggtgccg cttcccctct
    11281 tcatgccctt ctggatgcct agctggctca ggtcctggca agggaagctg taggtgagca
    11341 ggtcgatgtt cttcagtgtc ctcttgtaga ggtccctgat gtcgaaaatg ttgccctcct
    11401 tttcggacag cttgatggcg ttgtagatga tcttcagctc gtcatccttc ttcctcttcc
    11461 agtatccgtt gctgacgggg ttcttgctgt tccaggacag ggtcttgttc tcgaggtagt
    11521 cgatcatctc ctctctgctg acggacttgt actcgagctt ggtgtggaag ttgttatgga
    11581 tggcctggta catgacgatg gcgggcacat accactcggc cagtcccacg atctcgtact
    11641 cgtccttcct cactttctcc agggcctttc tctgggcgcc aatgccggcg aaggcttcga
    11701 acactctcag cttctttgtt ttgttctcca ctttgctgtc cctagccacc ttcctcttct
    11761 tcttggggtc agccctgctg tctccaccga gctgagagag gtcgattctt gtttcataga
    11821 gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta gaggtgtacc
    11881 gctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc aagttggtca
    11941 gagtaaacaa gtggataatg ttttctgcct gctccctgat gggcttatcc ctgtgcttat
    12001 tgtaagcaga aagcacctta tcgaggttag cgtcggcgag gatcactctt ttggagaatt
    12061 cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg ttgttccacg aacagctgct
    12121 tctgctcatt atcttcggga gaccctttga gcttttcata gtggctggcc agatacaaga
    12181 aattaacgta tttagagggc agtgccagct cgttaccttt ctgcagctcg cccgcactag
    12241 cgagcattcg tttccggccg ttttcaagct caaagagaga gtacttggga agcttaatga
    12301 tgaggtcttt tttgacctct ttatatcctt tcgcctcgag aaagtcgatg gggttttttt
    12361 cgaagcttga tcgctccatg attgtgatgc ccagcagttc cttgacgctt ttgagttttt
    12421 tagacttccc tttctccact ttggccacaa ccagtacact gtaagcgact gtaggagaat
    12481 cgaatccgcc gtatttcttg gggtcccaat cttttttgcg tgcgatcagc ttgtcgctgt
    12541 tccttttcgg gaggatactt tccttggaga agcctccggt ctgtacttcg gtctttttaa
    12601 cgatgttcac ctgcggcatg gacaggacct tccggactgt cgcgaaatcc ctacccttgt
    12661 cccacacgat ttctcctgtt tctccgtttg tttcgataag tggtcgcttc cgaatctctc
    12721 cattggccag tgtaatctcg gtcttgaaaa aattcataat attgctgtaa aagaagtact
    12781 tagcggtggc cttgcctatt tcctgctcag actttgcgat cattttccta acatcgtaca
    12841 ctttatagtc tccgtaaaca aattcagatt caagcttggg atattttttg ataagtgcag
    12901 tgcctaccac tgcattcagg taggcatcat gcgcatggtg gtaattgttg atctctctca
    12961 ccttataaaa ctgaaagtcc tttctgaaat ctgagaccag cttagacttc agagtaataa
    13021 ctttcacctc tcgaatcagt ttgtcatttt catcgtactt ggtgttcatg cgtgaatcga
    13081 gaatttgggc cacgtgcttg gtgatctggc gtgtctcaac aagctgcctt ttgatgaagc
    13141 cggctttatc caactcagac aggccacctc gttcagcctt agtcagatta tcgaacttcc
    13201 gttgtgtgat cagtttggcg ttcagcagct gccgccaata atttttcatt ttcttgacaa
    13261 cttcttctga ggggacgtta tcactcttcc ctctattttt atcggatctt gtcaacactt
    13321 tattatcaat agaatcatct ttgagaaaag actggggcac gatggcatcc acgtcgtagt
    13381 cggagagccg attgatgtcc agttcctgat ccacgtacat gtccctgccg ttctgcaggt
    13441 agtacaggta gagcttctca ttctgaagct gggtgttttc aactgggtgt tccttaagga
    13501 tttgggaccc cagttctttt ataccctctt caatcctctt catcctttcc ctactgttct
    13561 tctgtccctt ctgggtagtt tggttctctc gggccatctc gataacgata ttctcgggct
    13621 tatgccttcc cattactttg acgagttcat ccacgacctt aacggtctgc agtattccct
    13681 ttttgatagc tgggctacct gcaagattag cgatgtgctc gtgaagactg tccccctggc
    13741 cagaaacttg tgctttctgg atgtcctcct taaaggtgag agagtcatca tggatcaact
    13801 gcatgaagtt ccggttggca aatccatcgg acttaagaaa atccaggatt gtctttccac
    13861 tctgcttgtc tcggatccca ttgatcagtt ttcttgacag ccgcccccat cctgtatatc
    13921 ggcgcctctt gagctgtttc atgactttgt cgtcgaagag atgagcgtaa gttttcaagc
    13981 gttcttcaat catctcccta tcttcaaaca acgtaagggt gaggacaatg tcctcaagaa
    14041 tgtcctcgtt ctcctcattg tccaggaagt ccttgtcttt aatgattttc aggagatcgt
    14101 gatacgttcc cagggatgcg ttgaagcgat cctccactcc gctgatttca acagagtcga
    14161 aacattcaat ctttttgaaa tagtcttctt tgagctgttt cacggtaact ttccggttcg
    14221 tcttgaagag gaggtccacg atagctttct tctgctctcc agacaggaat gctggctttc
    14281 tcatcccttc tgtgacgtat ttgaccttgg tgagctcgtt ataaactgtg aagtactcgt
    14341 acagcagaga gtgtttagga agcacctttt cgttaggcag atttttatca aagttagtca
    14401 tcctttcgat gaaggactgg gcagaggccc ccttatccac gacttcctcg aagttccagg
    14461 gagtgatggt ctcttctgat ttgcgagtca tccacgcgaa tctggaattt ccccgggcga
    14521 gggggcctac atagtagggt atccgaaatg tgaggatttt ctcaatcttt tccctgttat
    14581 ctttcaaaaa ggggtagaaa tcctcttgcc gcctgaggat agcgtgcagt tcgcccaggt
    14641 gaatctggtg ggggatgctt ccattgtcga aagtgcgctg tttgcgcaac agatcttctc
    14701 tgttaagctt taccagcagc tcctcggtgc cgtccatttt ttccaagatg ggcttaataa
    14761 atttgtaaaa ttcctcctgg cttgctccgc cgtcaatgta tccggcgtag ccatttttag
    14821 actgatcgaa gaaaatttcc ttgtacttct caggcagttg ctgtctgaca agggccttca
    14881 gcaaagtcaa gtcttggtgg tgctcatcat agcgcttgat catactagcg ctcagcggag
    14941 ctttggtgat ctccgtgttc actcgcagaa tatcactcag cagaatggcg tctgacaggt
    15001 tctttgccgc caaaaaaagg tctgcgtact ggtcgccgat ctgggccagc agattgtcga
    15061 gatcatcatc gtaggtgtct ttgctcagtt gaagcttggc atcttcggcc aggtcgaagt
    15121 tagatttaaa gttgggggtc agcccgagtg acagggcgat aagattacca aacaggccgt
    15181 tcttcttctc cccagggagc tgtgcgatga ggttttcgag ccgccgggat ttggacagcc
    15241 tagcgctcag gattgctttg gcgtcaactc cggatgcgtt gatcgggttc tcttcgaaaa
    15301 gctgattgta agtctgaacc agttggataa agagtttgtc gacatcgctg ttgtctgggt
    15361 tcaggtcccc ctcgatgagg aagtgtcccc gaaatttgat catatgcgcc agcgcgagat
    15421 agatcaaccg caagtcagcc ttatcagtac tgtctacaag cttcttcctc agatgatata
    15481 tggttgggta cttttcatgg tacgccacct cgtccacgat attgccaaag attgggtggc
    15541 gctcgtgctt tttatcctcc tccaccaaaa aggactcctc cagcctatgg aagaaagagt
    15601 catccacctt agccatctca ttactaaaga tctcctgcag gtagcagatc cgattctttc
    15661 tgcgggtata tctgcgccgt gctgttcttt tgagccgcgt ggcttcggcg gtttccccgg
    15721 agtcgaacag gagggcgcca atgaggttct tctttatgct gtggcgatcg gtattgccca
    15781 gaactttgaa ttttttgctc ggcaccttgt actcgtccgt aatgacggcc cagccgacgc
    15841 tgtttgtgcc gatggcgagc ccaatggagt acttcttgtc cattccgcgg cccaccttcc
    15901 tcttcttctt gggggccatc ttgtcatcgt catccttgta atcgatgtca tgatctttat
    15961 aatcaccgtc atggtctttg tagtccataa ctttttttgt aattgtaaat agtaattgta
    16021 atgttgtttg ttgtttgttg ttgttggtaa ttgttgtaaa aatactttaa agagcctgct
    16081 tttttgtaca aacttgttga tgatccctgt taatcagaaa aactcagatt aatcgacaaa
    16141 ttcgatcgca caaactagaa actaacacca gatctagata gaaatcacaa atcgaagagt
    16201 aattattcga caaaactcaa attatttgaa caaatcggat gatatctatg aaaccctaat
    16261 cgagaattaa gatgatatct aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa
    16321 cagaatctaa accaaagaac atatacgaaa ttgggatcga acgaaaacaa aatcgaagat
    16381 tttgagagaa taaggaacac agaaatttac cttgatcacg gtagagagaa ttgagagaaa
    16441 gtttttaaga ttttgagaaa ttgaaatctg aattgtgaag aagaagagct ctttgggtat
    16501 tgttttatag aagaagaaga agaaaagacg aggacgacta ggtcacgaga aagctaaggc
    16561 ggtgaagcaa tagctaataa taaaatgaca cgtgtattga gcgttgttta cacgcaaagt
    16621 tgtttttggc taattgcctt atttttaggt tgaggaaaag tatttgtgct ttgagttgat
    16681 aaacacgact cgtgtgtgcc ggctgcaacc actttgacgc cgtttattac tgactcgtcg
    16741 acaaccacaa tttctaacgg tcgtcataag atccagccgt tgagatttaa cgatcgttac
    16801 gatttatatt tttttagcat tatcgtttta ttttttaaat atacggtgga gctgaaaatt
    16861 ggcaataatt gaaccgtggg tcccactgca ttgaagcgta tttcgtattt tctagaattc
    16921 ttcgtgcttt atttcttttc ctttttgttt ttttttgcca tttatctaat gcaagtgggc
    16981 ttataaaatc agtgaatttc ttggaaaagt aacttcttta tcgtataaca tattgtgaaa
    17041 ttatccattt cttttaattt tttagtgtta ttggatattt ttgtatgatt attgatttgc
    17101 ataggataat gacttttgta tcaagttggt gaacaagtct cgttaaaaaa ggcaagtggt
    17161 ttggtgactc gatttattct tgttatttaa ttcatatatc aatggatctt atttggggcc
    17221 tggtccatat ttaacactcg tgttcagtcc aatgaccaat aatatttttt cattaataac
    17281 aatgtaacaa gaatgataca caaaacattc tttgaataag ttcgctatga agaagggaac
    17341 ttatccggtc ctagatcatc agttcataca aacctccata gagttcaaca tcttaaacaa
    17401 gaatatcctg atctgaagaa tgtggaggct ttagtccctt ggatacttgg gaggctgtgg
    17461 aagaacagaa acgagctggt gcttaaaggg agggaatttg gaaccaatga ggtattagta
    17521 aggacacaag aagatgcaga tgagtggatt agaaggaaag aggctcagaa tgtaaggaaa
    17581 gcaccaacca caacaccgtc gggacagacg agaaacatgg gaagctccac cacaatcttg
    17641 ggttaagtgc aactttgatg gggcatggcc aacagaagga ttaaaatgtg gcttagggtg
    17701 ggtgcttcgc gatcatacag ggaaggtgtt atggttaggt gcaagagctg tggtaaaagt
    17761 aagaagcgtg ctggaagtag aagtggaggc tcttagatgg gctgtgctgt cattatcccg
    17821 attcaattat aggaagatca tttttgaggt ggattctcag caacttgtgt ctttggttac
    17881 atgaaagtta tgcttgtcaa gtctcaatcc aattatccaa gacataaagt atctacttag
    17941 caagtttgag gattttatgc ttgtgcatac aagccgagaa ggaaatggag tggcagatag
    18001 aatagctaag gaatctcttt cttttgagaa ttatgatcta aagttgtatt ctattgtacc
    18061 aatttgggtt aaaagctctg ttgagctaga ctcatatgca tgcggccgcg ttcctaacac
    18121 ctggagaacc ttttatgtac ttcacaaccc tcaatgctgc ttccaggtgt gactgtttgg
    18181 gttgctgcat gaattgactc agcacctgca ctgcaaagct gatatctggc cttgtgattg
    18241 tcaagtagag aagctttccc accagtctct gataggaacc cacatcctct aactctgcat
    18301 catcagtctt tcctaagtgc ttgtcatatt ctacagtagt cagcctttgg ttctgttcca
    18361 ttggtgttga cactggtctg cagcctccca gaccaacacc tgatatcagt tccagtgcat
    18421 acttcctctg gttcagtagg attccctttt ctgatctcag cacctcaatg cctaagaagt
    18481 attttagttc tcccaaatct ttcatcttga aatgctgatg cagggttgcc tttgcttctg
    18541 aaatcaaaac attgctgctg cctgttatta acagatcatc cacataaatc aggattatga
    18601 caaggtcagt cccctctctt ttggtaaaca aggagtgatc ataagcactt tgcataaaac
    18661 cagcctgcat aaggacagtg gtaagcttga tgttccactg ccttgatgct tgctttaaac
    18721 catagaggga ttcaacagcc tgcacacttt ggtctcccct tggctgtgaa aaccctgagt
    18781 acgggaggag gatctcggac cgaagagtac aagcttatcc tgaacggtaa aaccctgaaa
    18841 ggtgaaacca ccaccgaagc tgttgacgct gctaccgcgg aaaaagtttt caaacagtac
    18901 gctaacgaca acggtgttga cggtgaatgg acctacgacg acgctaccaa aaccttcacg
    18961 gtaaccgaag gtggtggtag cggtggtggt actagtccaa aaacaaggag gagaccgcga
    19021 agatcacaac ggaaaaggcc gcctacgcca tggccgtaag tttgagctcg aatttccccg
    19081 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
    19141 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
    19201 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
    19261 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
    19321 tgttacgaag ctttcgttga acaacggaaa ctcgacttgc cttccgcaca atacatcatt
    19381 tcttcttagc tttttttctt cttcttcgtt catacagttt ttttttgttt atcagcttac
    19441 attttcttga accgtagctt tcgttttctt ctttttaact ttccattcgg agtttttgta
    19501 tcttgtttca tagtttgtcc caggattaga atgattaggc atcgaacctt caagaatttg
    19561 attgaataaa acatcttcat tcttaagata tgaagataat cttcaaaagg cccctgggaa
    19621 tctgaaagaa gagaagcagg cccatttata tgggaaagaa caatagtatt tcttatatag
    19681 gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc gcttagataa gaaaacgaag
    19741 ctgagtttat atacagctag agtcgaagta gtgattgacg gaaagatgta tgggcttgtt
    19801 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
    19861 accgagtcgg tgcttttttt ggtttaggaa agctttcgtt gaacaacgga aactcgactt
    19921 gccttccgca ca
//
