LOCUS       sequence-343659-        5645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp12
FEATURES             Location/Qualifiers
     source          1..5645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2956..3055)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3072..3091)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3073..3091)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3096..3112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3096..3112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3543..4334
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4427..5015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4916..4935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5169..5186
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5345..5372)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5464..5550)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5599..5621
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5613..5630
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5614..5630
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct ctgcggatgc atcaacgttt ttaaaccggg tttgcggtgt aagtgcagcc
      181 cgtcttacac cgtgcggcac aggcactagt actgatgtcg tctacagggc ttttgatatt
      241 tacaacgaaa aagttgctgg ttttgcaaag ttcctaaaaa ctaattgctg tcgcttccag
      301 gagaaggatg aggaaggcaa tttattagac tcttactttg tagttaagag gcatactatg
      361 tctaactacc aacatgaaga gactatttat aacttggtta aagattgtcc agcggttgct
      421 gtccatgact ttttcaagtt tagagtagat ggtgacatgg taccacatat atcacgtcag
      481 cgtctaacta aatacacaat ggctgattta gtctatgctc tacgtcattt tgatgagggt
      541 aattgtgata cattaaaaga aatactcgtc acatacaatt gctgtgatga tgattatttc
      601 aataagaagg attggtatga cttcgtagag aatcctgaca tcttacgcgt atatgctaac
      661 ttaggtgagc gtgtacgcca atcattatta aagactgtac aattctgcga tgctatgcgt
      721 gatgcaggca ttgtaggcgt actgacatta gataatcagg atcttaatgg gaactggtac
      781 gatttcggtg atttcgtaca agtagcacca ggctgcggag ttcctattgt ggattcatat
      841 tactcattgc tgatgcccat cctcactttg actagggcat tggctgctga gtcccatatg
      901 gatgctgatc tcgcaaaacc acttattaag tgggatttgc tgaaatatga ttttacggaa
      961 gagagacttt gtctcttcga ccgttatttt aaatattggg accagacata ccatcccaat
     1021 tgtattaact gtttggatga taggtgtatc cttcattgtg caaactttaa tgtgttattt
     1081 tctactgtgt ttccacctac aagttttgga ccactagtaa gaaaaatatt tgtagatggt
     1141 gttccttttg ttgtttcaac tggataccat tttcgtgagt taggagtcgt acataatcag
     1201 gatgtaaact tacatagctc gcgtctcagt ttcaaggaac ttttagtgta tgctgctgat
     1261 ccagctatgc atgcagcttc tggcaattta ttgctagata aacgcactac atgcttttca
     1321 gtagctgcac taacaaacaa tgttgctttt caaactgtca aacccggtaa ttttaataaa
     1381 gacttttatg actttgctgt gtctaaaggt ttctttaagg aaggaagttc tgttgaacta
     1441 aaacacttct tctttgctca ggatggcaac gctgctatca gtgattatga ctattatcgt
     1501 tataatctgc caacaatgtg tgatatcaga caactcctat tcgtagttga agttgttgat
     1561 aaatactttg attgttacga tggtggctgt attaatgcca accaagtaat cgttaacaat
     1621 ctggataaat cagctggttt cccatttaat aaatggggta aggctagact ttattatgac
     1681 tcaatgagtt atgaggatca agatgcactt ttcgcgtata ctaagcgtaa tgtcatccct
     1741 actataactc aaatgaatct taagtatgcc attagtgcaa agaatagagc tcgcaccgta
     1801 gctggtgtct ctatctgtag tactatgaca aatagacagt ttcatcagaa attattgaag
     1861 tcaatagccg ccactagagg agctactgtg gtaattggaa caagcaagtt ttacggtggc
     1921 tggcataata tgttaaaaac tgtttacagt gatgtagaaa ctccacacct tatgggttgg
     1981 gattatccaa aatgtgacag agccatgcct aacatgctta ggataatggc ctctcttgtt
     2041 cttgctcgca aacataacac ttgctgtaac ttatcacacc gtttctacag gttagctaac
     2101 gagtgtgcgc aagtattaag tgagatggtc atgtgtggcg gctcactata tgttaaacca
     2161 ggtggaacat catccggtga tgctacaact gcttatgcta atagtgtctt taacatttgt
     2221 caagctgtta cagccaatgt aaatgcactt ctttcaactg atggtaataa gatagctgac
     2281 aagtatgtcc gcaatctaca acacaggctc tatgagtgtc tctatagaaa tagggatgtt
     2341 gatcatgaat tcgtggatga gttttacgct tacctgcgta aacatttctc catgatgatt
     2401 ctttctgatg atgccgttgt gtgctataac agtaactatg cggctcaagg tttagtagct
     2461 agcattaaga actttaaggc agttctttat tatcaaaata atgtgttcat gtctgaggca
     2521 aaatgttgga ctgagactga ccttactaaa ggacctcacg aattttgctc acagcataca
     2581 atgctagtta aacaaggaga tgattacgtg tacctgcctt acccagatcc atcaagaata
     2641 ttaggcgcag gctgttttgt cgatgatatt gtcaaaacag atggtacact tatgattgaa
     2701 aggttcgtgt cactggctat tgatgcttac ccacttacaa aacatcctaa tcaggagtat
     2761 gctgatgtct ttcacttgta tttacaatac attagaaagt tacatgatga gcttactggc
     2821 cacatgttgg acatgtattc cgtaatgcta actaatgata acacctcacg gtactgggaa
     2881 cctgagtttt atgaggctat gtacacacca catacagtct tgcagcttaa ttaagacgcg
     2941 atcgcggcct catgggccca gctttcttgt acaaagttgg cattataaaa aataattgct
     3001 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     3061 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     3121 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     3181 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     3241 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     3301 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     3361 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     3421 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     3481 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     3541 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     3601 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3661 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3721 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3781 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3841 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3901 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3961 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     4021 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     4081 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     4141 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     4201 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     4261 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     4321 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     4381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4561 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5101 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5161 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     5221 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     5281 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     5341 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     5401 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     5461 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     5521 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     5581 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     5641 ggccc
//
