LOCUS       sequence-343662-       10744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 expression vector for Anopheles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10744)
  TITLE     Marois lab kit for CRISPR and transgenesis in Anopheles and Aedes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDSAY-vasaCas9sv40
FEATURES             Location/Qualifiers
     source          1..10744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     256..278
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     270..287
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     271..287
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(337..461)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      2711..2720
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2720..2785
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2792..2812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2837..6937
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2837..6937
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6938..6985
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    7102..7184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7242..7311
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     regulatory      7826..7835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7832..7834,7835..7837,7838..8551)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8138..8157)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8485..8506
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8674..8795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8856..8955)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8985..9004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8986..9004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9009..9025)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9009..9025)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9127..9146)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9138..9947
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9205..9224)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10094..10682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10583..10602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      301 ccctacaggt cactaatacc atctaagtag ttgattcata gtgactggat atgttgtgtt
      361 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      421 tcattttacg tttctcgttc aacttttcta tacaaagttg gtaccggatc ccgatgtaga
      481 acgcgagcaa attcttttcc ttccatgaca gcagcagcta cagtgggaag ccgaacgtca
      541 gacgtgtttg acatgccgaa ctgggcggga aaattacagc gtgcgctttg ttttcaagca
      601 aatcacaact cgctgcaaac aaaaccgttg agaaattgat tgttttataa tttgtattgt
      661 attttatttg ttataataaa ctaaaaagac atactttttg catattttat acataaaaac
      721 atacatgcag cattataaaa cacatataaa ccctccctgt agagtcccgt atcgaaatct
      781 tccatcctag ttgcacagta cgacggacga gtaggccgtg tccgtgcaaa ttccagcttt
      841 tagcagtctt ttgctcggag cactcgcggc gagtcggagg tttctgctga ggtgcttagc
      901 gctaaattag ccaattgctt ttgcaagtga aataaccagc cgaatagtac ttcaaaactc
      961 aggtaagtga actagtttta tagaacaaat gtttgtttgt tagaagttag tgaagtgttt
     1021 gtgaaaaaaa tctctcattt cggcaaaact aacgtaactg atttcaaatt gaattattgt
     1081 tttgtgatgt tatattattt catccagttg attagtattt tcttagttat gttcaaaata
     1141 cagttaaatt aaatttcatt tcatttactc ataaaataat ctcttggctt atttaatttt
     1201 tctcgaattc gcttgtattg ttcagtagca cgcgccattc gccctttgtt tcattttgta
     1261 cctgctccca ctaacacact ggcagtgcga aacaaaagcc ttcgcacgcg ttgctggtat
     1321 tagagtgtgt gcgtgtgtgt gttgagcgct ctgtcaaaat cggctgttgc cgccggtacc
     1381 gaaattgcct gttcgcacgc tgttcgtaaa cattccgtgg tgtgtatcgt gtgttgtgca
     1441 tgttgcgcgc ctcccccctt ttgatagcag gctgccgtgg ctgccgtggt gtgtggcgca
     1501 gttgagtttt tggattaatt ttctaaggaa atggcacgag aagagcggtg gcagtgtgtt
     1561 ggtttgctct gtcccttcct ttctgtgtga agtgttctta cagcacagca cgtatccacc
     1621 accgcacaca gagcaggcaa ggaagtggaa gtgaacaagt gtgctgcgca tgcatgtgtg
     1681 tggggggcat tttagctgag atcgtcgtta tttgagaagc ggtatagggg ccagtcggtg
     1741 tcgacgtacg gaagcggttt agttttaatc caagcgtatc ccgtcgtgga gtggttgtgt
     1801 ggctctgtgt gctctcatat cagttccaga gtgaggttag tagaatcaca gtccttggcc
     1861 tttttcgtta caagatatcc agaaggatgg cgttatttcc acagcttacc atggtgctct
     1921 tgtttgctcg aatcagggga gaaaaacagt ttcgtgtttc atgaaccgca gttggcactg
     1981 gagcggattc aaaagtcttc gatatgcaat agataagaga gtcgttgggg catagttggg
     2041 aagcctttcc gagatgtgga gtttccgaga ggagaaatgg tgctttcgtg cacgttccgg
     2101 gacagcgggc cccgcgaaga gcatctcgtt gtcgttcatc cggcaataat tgatgcgaaa
     2161 agcgcgcgcg ccactggctt agcgcagtgt acacagtgat attcacctac acacacagag
     2221 gcacacgcct tcacacgcgc gcgtgcttca aaggctactt cggtggcggt gtgtgaggtc
     2281 gcttgcaatg gacaatgaaa atttcgctgg aaaataccat cgtctcttta ggttgcaatg
     2341 ggtgcgggta gagcggtggt cgtcgatatt ggtggtgtag tgtgtgtgtg tgtgtgtgtg
     2401 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     2461 tgtgtgtgtg tgtgtgcaac ggcaattatt ttttgtaata tttcgaccat ctttctttct
     2521 ctctctccac gtgctgctgc tgttgctgct gctgctgcat tgcatgttcc actattcctc
     2581 tcggtttgtg cctgcggacg ccattgctag tcgaaagaga gtcgccgtta gtcgcgcttc
     2641 gagcaacgga cacgtttttt ggttgaaacc aacagctttt ttcatcttcg ggagacacac
     2701 agatcccggt gccaccatgg actataagga ccacgacgga gactacaagg atcatgatat
     2761 tgattacaaa gacgatgacg ataagatggc cccaaagaag aagcggaagg tcggtatcca
     2821 cggagtccca gcagccgaca agaagtacag catcggcctg gacatcggca ccaactctgt
     2881 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     2941 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     3001 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     3061 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     3121 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     3181 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     3241 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     3301 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     3361 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     3421 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     3481 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     3541 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     3601 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     3661 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     3721 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     3781 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     3841 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     3901 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     3961 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     4021 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     4081 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     4141 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     4201 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     4261 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     4321 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     4381 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     4441 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     4501 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     4561 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     4621 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     4681 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     4741 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     4801 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     4861 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     4921 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     4981 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     5041 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     5101 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     5161 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     5221 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     5281 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     5341 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     5401 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     5461 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     5521 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     5581 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     5641 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     5701 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     5761 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     5821 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     5881 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     5941 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     6001 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     6061 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     6121 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     6181 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
     6241 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     6301 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     6361 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     6421 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     6481 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     6541 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     6601 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     6661 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     6721 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     6781 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
     6841 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     6901 cgagacacgg atcgacctgt ctcagctggg aggcgacaaa aggccggcgg ccacgaaaaa
     6961 ggccggccag gcaaaaaaga aaaagtaaga attctagaca taatcagcca taccacattt
     7021 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     7081 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     7141 aatagcatca caaatttcac aaataaagca tttttcttca ctgcattcta gttgtggttt
     7201 gtccaaactc atcaatgtat ctcgacgatg taggtcacag tctcgaagcc gcggtgcggg
     7261 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     7321 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     7381 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     7441 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcaat
     7501 tcctgcagac ttccggtatc tcgcgtttgt ttgatcgcac ggttcccaca atggttaatt
     7561 cgagctcgcc cggggatcta attcaattag agactaattc aattagagct aattcaatta
     7621 ggatccaagc ttatcgattt cgaaccctcg accgccggag tataaataga ggcgcttcgt
     7681 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
     7741 gcaaataaac aagcgcagct gaacaagcta aacaatcggg gtaccgctag agtcgacggt
     7801 accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     7861 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     7921 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     7981 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     8041 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     8101 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     8161 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     8221 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     8281 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     8341 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     8401 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     8461 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     8521 cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc
     8581 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     8641 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8761 agttgtggtt tgtccaaact catcaatgta tcttaaagct tatcgatacg cgtacggcgc
     8821 gcctagagcg gccgccaccg cggtggagct cgagtaccca gctttcttgt acaaagttgg
     8881 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     8941 taaaatcatt atttgccatc cagctgcagg gcggccgcga tatcccctat agtgagtcgt
     9001 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     9061 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     9121 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     9181 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     9241 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     9301 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     9361 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     9421 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     9481 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     9541 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     9601 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     9661 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     9721 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     9781 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     9841 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     9901 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     9961 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
    10021 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
    10081 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10141 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10201 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10261 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10321 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10381 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10441 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10501 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10561 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10621 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10681 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10741 tgtt
//
